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USJ Theses and Dissertations

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  • Although there is a substantial body of research on the second language acquisition of adults, there is little specific research on the learning experiences of senior and very senior adults. This thesis investigates and discovers the experience of being a senior from a traditional Confucian Heritage Culture aged between 55 and 75 years old, learning English as a foreign language through various interventions, including, the introduction of an adapted version of synthetic phonics to improve pronunciation, alongside the use of andragogical and geragogical principles to accommodate and encourage the development of agency and self-directed learning. This research adopted a case study methodology to investigate the lived experiences of seniors, and investigated the participants’ subjective constructions of the situation, learning experiences, challenges, circumstances, needs, and wants with regard to the situation. Therefore, an open and exploratory case study design was selected to understand the participants and report the findings. Furthermore, this thesis identifies the challenges faced by senior and very senior learners who are post-work and post-family rearing to make recommendations from the findings to complement, enhance and empower their learning

  • This thesis explores language teaching and language acquisition by multilingual learners using a Variation Theory approach and multilingual teaching in a university setting in Macao, China. It includes three case studies applied to students of the Spanish language in the introductory level which took place from late August to early December of the year 2017. The first study describes Macao’s multilingual language learners in the University of Macao in 2017. Based on the LEAP-Q questionnaire, a questionnaire was created to inquire all Spanish language students about their languages´ background, their motivations to learn new languages, as well as their learning strategies. The second study shows how the usage of Variation Theory techniques and multilingual teaching techniques boosted the teaching and the learning during the semester. This study employs a case study methodology, by analysing in-class multiple interactions gathering information on how multilinguals´ language background affects the pedagogical process. It analyses a total of 28 classes of 1 hour and 15 minutes. The third study presents the analysis of a questionnaire to 82 students of the initial level of Spanish language in the University of Macao, along with the analysis of interviews from 10 selected multilingual students about their linguistic background and how they experienced the semester. These interviews collected more information about the effectiveness of the Variation Theory in the semester in terms of in-class teaching and learning. From the triangulation of these three studies, some conclusions have been drawn about the advantages of using Variation Theory and multilingual teaching techniques for multilingual students, for the language teacher and ultimately also into the curricular design of foreign language teaching. In sum, that the linguistic background of students plays a major role in how they acquire a new language and, that applying Variation Theory techniques can be an immensely effective technique in a language classroom setting; suggesting that multilingual students will gain from being previously identified and placed in a separate class where these variation techniques were applied. Since this thesis focuses solely on an introductory language course, there is ground to explore this same approach on more advanced multilingual language learners

  • Government service mini-programs have become an integral component of eGovernment in the Greater Bay Area, and successful eGovernment is necessary for building a smart city. Service quality and citizens' trust play a vital role in urban integration and in-depth cooperation in the Bay Area. The ubiquitous nature of mini-programs based on WeChat and Alipay provides excellent flexibility in accessing government services. Technology advantages, mutual recognition of cross-border data, and online transactions bring value and benefits to citizens. However, the mechanism of mini-program adoption has not been elaborated. Homogenization, conflict of regulations, and policy effectiveness are issues of great concern. This study employed Self-Determination Theory and Motivation Theory, proposed an empirical model based on the extended SOR paradigm, and aimed to identify the critical factors determining the intention of government service mini-program adoption from the user’s perspective. Six hundred and nine valid samples were collected from Macau, Hong Kong, Guangzhou, and Shenzhen through online survey platforms. The findings suggested that service quality, trust in eGovernment, ubiquity, and social influence constituted the determinants of intention to adopt. Service quality and ubiquity were salient determinants, and a great extent of service quality and ubiquity could promote perceived value and intention. Citizens' trust in government service mini-programs was reasonable, where benevolence, integrity, and competence were crucial indicators of trust. Social influence amplified and transmitted risk perception while perceived risk significantly reduced intention. Perceived value positively associated with the four determinants and enhanced user intention; it acted as a mediator with high explanatory power in the model. Government support received positive ratings from citizens; it negatively regulated the relationship between intention and the determinants respectively, implying that excessive intervention from the government could lead to inhibition. Finally, we proposed relevant implications and suggestions for the GBA government agents and policymakers

  • According to the United Nations (UN) World Urbanization Prospects 2018, cities are growing in both size and number, posing challenges for sustainable development. With urbanization, the increase of impermeabilization and landscape fragmentation urges for territorial planning and resources management. To promote resilient and resourceful cites, strategic planning where nature-based solutions (NBS) are included into the built environment, counteracting the negative effects of urbanization through the provision of ecosystem services, should be considered. Green roofs (GR) are an example of NBS that provide a panoply of ecosystem services at the level of the building and city, besides contributing for climate change mitigation and adaptation. This NBS can be included in the urban planning agenda in the new building or as a retrofit solution in the existing built environment. Macao SAR (here mentioned as Macao) is the second more densely populated city in the world, according to the UN Department of Economic and Social Affairs Population Division. The present study aims to assess the inclusion of GR in Macao and assess the ecosystem services that they may provide in a holistic approach aligned with the city’s sustainable development. For this purpose, an extensive GR implemented in Taipa Island of Macao was monitored in terms of associated biodiversity and carbon cycle assessment. Further on, it was carried out a comparison between this GR and a conventional roof (CR) in terms of surface thermal buffering. The results of this study have shown to be very promising in terms of the applicability of GR in Macao and on the relevant ecosystem services that they can provide. First, data loggers were installed in the GR and CR to assess the impact on temperature mitigation. The GR had a significant effect in decreasing and buffering temperature at the surface, with temperatures of 35.6 ºC in the GR substrate comparing with temperatures of 57.7 ºC in the CR surface. The results show that GR can be a cost-efficiency strategy to reduce heating of buildings and their associated environmental and economic costs with cooling. Second, the biodiversity of plants and animals was assessed using a combination of methods. It was observed a high level of biodiversity under a low maintenance and low disturbance of GR vegetation, with 23 species of plants and 34 species of animals identified. This shows that GR can contribute to increase biodiversity in cities. Third, an experiment was run with leaf litter bags to investigate the role of macrofauna (e.g., insects) in the decomposition of organic matter. With macrofauna activity, the decomposition rate increased 17 % when compared to decomposition rates without macrofauna, suggesting that the animals harboured in GR, in particular insects, play a key role in organic matter decomposition and thus on the carbon cycle in cities. This work is, to our knowledge, the first empirical GR study in Macao. The results support the application of GR in Macao for temperature mitigation, biodiversity increase and carbon sequestration, promoting the integration of this NBS in the future urban planning and policy of the city. More generally, the work supports the use of GR as an important NBS to mitigate the impact of climate change in urban settings

  • The demand for plastic has led to enormous plastic waste in the environment, which persist and negatively impact the ecosystems. Polyethylene terephthalate (PET) is one of the most common thermoplastic polymers available on the market. The concerns about plastic waste generated an interest in strategies to enhance its biodegradation and finding alternative polymers. In this work was investigated the possibility of using bacteria to degrade PET and to produce bioplastics (Polyhydroxyalkanoates, PHAs). Finally, the integration of the two processes was tested. Overall, the work aimed to investigate the potential to recycle PET into bioplastic using bacteria. The potential of bacterial consortia from various environmental samples to degrade PET granules in liquid matrix was investigated. . The results revealed maximum PET granules degradation of 1.1 % by one of the tested consortia. PET degradation intermediate terephthalic acid (TPA) was not detected at the end of 55 days. Fourier-transform infrared spectroscopy (FTIR) results showed major spectral peak shifts and bends on PET chemical structure compared to non-inoculated control. The biodegradation of PET films buried in the soil (A), with mangrove plants (B), and bioaugmented with a bacterial consortium (C) was also investigated. The experiments were conducted for 270 days at ambient conditions. The results revealed no difference between treatments in the degradation, with a maximum weight loss of 0.118 % in the bioaugmented treatment. Nevertheless, Scanning Electron Microscope (SEM) and FTIR results indicated significant surface changes, spectral peak shifts, and stretches in PET chemical structures. Bacterial consortia isolated from the soil of the experimental treatments were assessed for degradation of PET monomers, TPA and monoethylene glycol (MEG), and intermediate Bis(2-hydroxyethyl) terephthalate (BHET). The consortia were inoculated in flasks containing minimal media with 1000 mg/L TPA or BHET or1113 mg/L MEG as the sole carbon source. Results showed complete degradation of TPA and significant degradation of BHET (96.09%), and MEG (83.65%) by the consortia. In the second part of the study, bacteria were isolated from various environmental samples and screened for PHA production using Sudan Black B staining on colonies and smeared glass slides. Transmission Electron Microscope images were captured to confirm the intracellular PHA inclusions. A total of 35 isolates were screened for PHA, and 22 showed positive staining. The isolate showing higher levels of PHA synthesis (EC2-30-3) was identified based on 16S rRNA gene sequence as Bacillus sp. and selected for PET monomers degradation and fermentation cultures for PHA production. It was cultured in minimal (Moreira et al., 2013) media with 1000 mg/L TPA and 1113 mg/L MEG as the carbon source for eight days. The isolate grew better in media containing MEG, which was selected as a substrate model for PHA fermentation. To integrate PET monomers biodegradation and production of PHA, the isolate was cultured in 0.2 % MEG. A control with 0.2 % of glucose was prepared, and the cultures were incubated for 96 hours. Bacillus sp. EC2-30-3 showed higher PHA accumulation in media supplied with MEG (40.31%) than glucose (25.53%). This is the first report showing that Bacillus sp. uses PET monomer as carbon source to produce a biopolymer. FTIR results of the extracted PHA identified its functional units as C–H, CH3, C=O, and C–O groups. The absorption bands obtained are closely related to the structure of PHB. The study thus confirmed the ability of the isolated bacteria to degrade PET monomers and produce biopolymers. The results of this work open the possibility for upscaling the use of bacteria to mitigate the impact of PET on the environment while producing environmentally friendly bioplastics

  • Seagrasses play a critical role in coastal ecosystems worldwide, providing various ecosystem services based on their region and genus. In Southeast Asia, where seagrass biodiversity and extents are at their highest, the livelihoods and food security of many coastal communities depend on these plants. Despite their ecological and economic importance, seagrasses face global threats from human activities such as pollution and land use changes. Enhalus acoroides, a widely distributed seagrass species in the tropical Indo-Pacific region, is particularly valuable for coastal management and conservation efforts due to its size and provision of various ecosystem services. Although previous research has indicated that it is less sensitive to environmental changes than other tropical seagrass species, recent reports highlight its vulnerability to siltation and eutrophication. This dissertation aimed to examine how Enhalus responds and adapts to changes in light availability, taking into account both morphological adaptation and phenotypic plasticity. Field surveys, reciprocal transplantation field experiments, and investigations of sexual reproductive effort were conducted in the Bolinao-Anda Reef system (NW Philippines) to evaluate the impact of long-term environmental changes on Enhalus populations. The findings of this study revealed that Enhalus has the capacity to adapt its traits and survive changes in depth, light gradients, and different habitat types. This is evidenced by larger shoots in low-light environments, which is apparently a response to the reduction in light availability, as evidenced in both in situ and experimental setups. Larger leaf surface area in light-reduced setups also had higher concentration of chlorophylls a and b pigments. Transplants from light-reduced environments, although morphologically large, appeared more vulnerable (with low survival values) to environmental changes associated with translocation. Being morphologically large is therefore likely a stress response to light reduction, allocating more energy on light harvesting than sexual reproduction. Reciprocal transplantation experiments indicated a high survival rate, suggesting the potential of Enhalus for use in rehabilitation. However, despite having wider plasticity to adapt to light-limitation, they can be wiped out when threshold is reached. This thesis underscores the need for further research on Enhalus' response to stressors, genetic variation, and adaptive capacity to address conservation and management challenges

  • "Adult neurogenesis, i.e., the production of new neurons in the adult brain, has been studied intensively in the past years, both in humans and in animal models, as the understanding of this process can have major clinical implications. The study of neurogenesis in fish has been receiving more attention as, unlike mammals, they possess remarkably high levels of adult neurogenesis and a high capability for neuronal regeneration and replacement where neuronal death has occurred. Less is known, however, on the importance of adult neurogenesis for behavioural plasticity, i.e., for the capacity to change behaviour according to context. As a product of the brain, behaviour relies on functional neuronal networks and it may be expected that more permanent changes in behavioural states imply structural reorganization of neuronal circuits, with the integration of new neurons. Interestingly, the high level of brain plasticity of fish is paralleled by a high degree of behavioural plasticity, with many examples of species that change, either reversibly or irreversibly, their behavioural phenotype during their lifetime, as illustrated by species with functional sex-change and alternative reproductive phenotypes. Flexibility in behaviour may thus require a reorganization of neuronal networks underlying these behaviours with recruitment of new neurons. In this thesis, the link between brain and behavioural plasticity was studied in a small marine fish that inhabits the Mediterranean and adjacent Atlantic coasts, the peacock blenny Salaria pavo. In this species, males adopt nests in rock crevices and attract females into the nest for egg laying, with the male taking care of the eggs until hatching. In some populations, a scarcity of nest sites drives smaller and young males to adopt an alternative reproductive tactic to reproduce. These “sneaker” males mimic the females’ morphology and reproductive behaviour in order to illude the larger nesting males and parasitically fertilize eggs during mating events. Sneaker males later transition into the nesting male phenotype, and this major behavioural transformation in the same animal, first courting males and afterwards courting females, may imply significant reorganization of brain areas associated with reproductive behaviour. During the study, a brain atlas for the species was developed and the main cell proliferation regions, i.e. niches of stem cells birth that may differentiate into cells of the nervous system, characterized. Proliferative areas were observed throughout the whole brain and paralleled the pattern described for other teleosts. Proliferative cells were abundant namely in areas like the olfactory bulbs (granular and glomerular), the anterior subdivision of the dorsomedial telencephalon (DMa), the dorsal and ventral part of the ventral subdivision of the dorsomedial telencephalon (DMvd and DMvv), the dorsal part of the dorsal subdivision of the dorsomedial telencephalon (DMdd), the posterior subdivision of the dorsolateral telencephalon (DLp), the posterior zone of the dorsal telencephalic area (DP), the preoptic area (POA), the dorsal, supracommissural and ventral nucleus of the ventral telencephalic area (Vd, Vs and Vv), the optic tectum and its periventricular grey zone (TeO and PGZ), the ventral zone of the periventricular hypothalamus (Hv), the cerebellum, mainly the molecular layer (CCeM) and the caudal lobe (LCa). A study of the brain nuclei activated during female courtship events using immediate early-genes suggested that some of the areas of the social behaviour network (SBN), a set of brain nuclei underlying the expression of social behaviour across vertebrates, are implicated in female courtship, in particular nuclei in the ventral telencephalic regions. This was followed by an experiment to investigate the possible link between cell proliferation and male tactic switch. Nest availability was manipulated to allow a fraction of sneaker males to adopt a nest and start the transition to nesting males. Ten days after the experiment, some of the smaller males had indeed started switching into nesting males, adopting a nest and starting to develop male secondary sexual characters. The pattern of brain proliferation was studied in these fish to try to confirm that the irreversible behavioural transition would be associated with the reorganization of brain nuclei, assuming that cell proliferation relates to neurogenesis and structural reorganization. Transitional males had elevated cell proliferation levels, as compared to males that remained sneakers, in the dorsolateral anterior and posterior telencephalic regions, thought to be homologous to the hippocampus in mammals. Cell proliferation levels were generally elevated in ventral and ventromedial telencephalic nuclei in both sneakers and transitional males, as compared with nesting males and females, areas considered to be homologous to nuclei of the amygdaloid complex of mammals. There was large variation in proliferation levels within transitional males, and in particular one male more advanced in the transition had higher numbers of BrdU-positive cells than the others. This suggests that a longer time-window for detecting the peak in brain cell proliferation associated with tactic transition in some fish may have been needed. Overall, the study supports the hypothesis that behavioural transition in males of this species is paralleled by an increase in cell proliferation in nuclei potentially relevant for the expression of reproductive behaviours, and establishes the peacock blenny as a new relevant model for the study of neuronal plasticity in vertebrates."

  • Listening to children’s voices is still not considered an essential part of education in some schools, including many in Asian countries. The authority of schools and teachers is still highly valued under the continued influence of Confucian Heritage Culture in many Asian schools, including a significant number in Macao. Teachers in international schools in Asian countries often experience some difficulties when communicating with young children because of their low English proficiency and the traditional views supported by many parents who grew up with the Confucian Heritage Culture, which encourages children to be quiet in the classroom to be good listeners. This Action research took fifteen months between two school years, 2018- 2019 and 2019-2020, with two groups of four and five-year-old students in a kindergarten classroom. Documentation posters were created for young children to use the next morning to reflect on their learning. The pedagogy of listening and pedagogical documentation from the Reggio Emilia approach were implemented to discover and record young children’s ideas and interests, work with daily documentation posters, and help them reflect on documentation posters to improve their learning and develop their higher-order thinking skills. Photos and videos, observation notes with the children’s comments, documentation posters, and reflective discussions were used as interventions to collect the children’s ideas and record their learning activities. The children learned to use documentation posters to remember, think, share, and improve their learning. The children’s comments from Learning Centres, recess, and reflective discussions were used to examine their understanding of learning and higher-order thinking skills. During one Pilot Cycle and three structured data collection cycles, the children demonstrated improvement in learning for each learning project and development of their thinking skills both with and without the teacher’s support. The children demonstrated higher-order thinking skills more often from Learning Centres and recess when they had to solve problems. They also demonstrated higher-order thinking skills more often during the whole group reflective discussions than in small group reflections, when a bigger number of children joined or when they had enough time to think. The thinking skills when children were reflecting were observed to concentrate on remembering and understanding as they focused on remembering and sharing the previous day’s work. The children’s other higher-order thinking skills did not show an increase in frequency during reflective discussions. However, the children demonstrated active engagement and a range of higher-order thinking skills when the teacher asked openended questions and provided support and comments to help them to connect their learning to their past experiences. Findings indicated that the children’s learning from each Learning Centre showed change and improvement during their play over time according to their interests, indicated by their material use and comments. The research was limited by its small number of participants within their age group due to convenience sampling and the children’s relatively limited ability to demonstrate higher-order thinking skills. This study has shown how teachers could help children use daily documentation posters to develop their learning and thinking skills by visualizing their ideas and the teacher’s important role in supporting children’s learning with active listening and support in the classroom

  • This dissertation is an attempt to analyze the twelve selected dreams written or reviewed by St. John Bosco in order to present the notion of an exemplary Salesian. These dreams inspired Don Bosco’s vocation, charism, and dealing with his life’s contingencies and correlate with his pastoral ministry for youth under the social-ecclesiastical context of the nineteenth century. The methodology for this research consists of two approaches: an investigation of their spiritual theology, and Morton T. Kelsey’s approach. In the first part, five dimensions of spiritual theology are explored: (1) the conformity to the truth, (2) the humility and the docility to the Divine will, (3) the discretion and the gravity of seeking the glory of God, (4) the liberty of Spirit with joy, and (5) the guidance of the Spirit. In the latter part, three issues inspired by Kelsey are explored: (1) the personal issue, including the exploration of thought and feeling; (2) the correlation between the dream content and the reality, including images and archetypal symbols associated with experience and memory; (3) the spiritual issue, including one’s openness to God and relation with Him. Through this present research, a model of an exemplary Salesian has been conceptualized, which considers three virtues: health, wisdom, and sanctity. This present study is expected to be beneficial to enhance the faithful’s spirituality. Moreover it is hoped it will provide a healthy stimulus for Salesians to seek to live an excellent Salesian religious life, and to the faithful be devoted Christians in this world as well for the sake of glorifying the Divine mercy

  • This dissertation is a comprehensive academic examination of the characteristics and complex historical progress within the Pentecostal and Charismatic Movement in Macau Protestant Churches. Since Macau hosted the first Pentecostal and Charismatic missionary, Thomas J. McIntosh, who entered China in 1907, the history of the Pentecostal and Charismatic Movement in Macau lacks a consistent and synthesized research until now. Thus, primary and secondary resources have been analyzed and reconstructed and historically interpreted to gain a better and deeper understanding of the emergence and development of the Pentecostal and Charismatic Movement within Macau’s Protestant Churches from the beginning up to the present day. This academic review makes an important contribution to Macau with respect to its historical development and will fill the gap in knowledge within the Global church history of the Pentecostal and Charismatic Movement. Key Words: Charismatic, Christianity in China, History of Missionaries in China, Macao, Macau, Pentecostalism, Protestant, T. J. McIntosh

  • Information and communication technologies (ICTs) are highly associated with the study of e-government, and many scholars believe that within the coming decades, government operation and policy decision-making cannot persist without the use of ICTs (Van Dijk, 2018). This thesis aims to generate a conceptual framework of the behavioral factors that could contribute to the acceleration of the implementation of e-government services in Macao SAR. Rather than regarding e-government services as a goal to be realized in traditional practice through evaluating the outcome, a process-oriented study was conducted. The e-government services are regarded as advanced tools in the 21st century to transform Macao into a smart city. The design of the process-oriented approach and the comparative study of four groups of Macao citizens' behavioral intentions are solidly supported by the research gaps identified in the literature review of e-government studies in an international perspective and the actual context of local Macao studies. Under the framework of the Theory of Planned Behavior (Ajzen, 1985, 1991), the behavioral factors of the general public and civil servants are investigated through a qualitative approach, and the findings are triangulated from various aspects. Firstly, a systematic literature review of TPB was conducted thoroughly to better understand the current study of e-government around the world. Secondly, a content and thematic analysis of the official documents and articles from local press media and research institutes related to the topic of e-government services was carried out to demonstrate a more comprehensive picture of the current problems of implementing iv and adopting e-services in Macao SAR. Observations in some government premises that provided e-services and 40 in-depth interviews were conducted to generate detailed and first-hand data. Key issues were extracted from the interviewees’ narratives and daily actual usages. Different conceptual models for different age groups and civil servant group were formed. Special attention was paid to analyzing the "hard-to-reach" groups' behavioral intentions. Research limitations identified from the previous literature were overcome partially in this study too. After comparing the similarities and differences, a new conceptual model of significant behavioral factors that affect the behavioral intentions in adopting e-government services was built. Results and findings from the analysis could be used to develop effective interventions by the government policymakers in responding to the behavioral change of the general public in the aspect of e-government services acceptance and adoption

  • The global living standard improved significantly in the last decades and China moved 800 million people out of poverty since 1980. However, production and consumption in their current forms are unsustainable in matters of resource and energy use and involved emissions with their significant ecological impact. The global human community committed itself in the COP21 Agreement of Paris 2015 to reduce Green House Gas (GHG) emissions based on National Determined Contributions (NDCs) in order to limit the increase of global average temperature below 2°C or better 1.5°C above pre industrial levels. This commitment entails a comprehensive transformation of the current social and economic system in view of decoupling economic growth from both resource extraction and GHG emissions, in view of fostering a resource sensitive and CO2 neutral Circular Economy (CE) based on Sustainable Development (SD). China submitted its first Intended Nationally Determined Contributions (INDCs) in 2015 with policies and measures affecting 15 major areas. In 2020, President Xi Jinping announced the commitment to peak China’s carbon dioxide emissions before 2030 and achieving carbon neutrality before 2060. Proper and Integrated Resource and Waste Management is central on the way to achieve the transformation into a CE. The State Council of the People’s Republic of China (PRC) released its plan “生活垃圾分类制度实施方案” to promote source separation of household waste on March 30, 2017 aiming at the recycling rate for household waste to reach 35% by 2020 for the selected cities. The first China’s Mandatory Waste Source Separation Law “廣州 市生活垃圾分類管理條例” was enforced by the city of Guangzhou on July 1st, 2018. One of the key strategic plans in China is the development of the Greater Bay Area (GBA) with its comprehensive development plan released on February 18, 2019. Following the INDCs by China, parts of the GBA Development Plan emphasize that the development of the CE systems and the implementation of extended producer responsibility (EPR), are effective principles to provide financial incentives in view of reducing embedded emissions in material and processes. The present research studied, analysed, and compared the MSW treatment strategies, rules, regulations, and retrievable data, which lead to MSW source separation and the reverse logistic of separated waste among 4 selected cities Guangzhou, Zhuhai, and the 2 S.A.R.s of Hong Kong and Macao. The experience and comparison from Guangzhou and Hong Kong revealed that a Top-down approach in environmental policy decision making is more efficient and is able to implement necessary policies faster. However, the experiences from Guangzhou and Zhuhai indicate, that also a more participatory implementation process is crucial, as it enables the involved stakeholders to express their experiences and opinions properly, which can lead to a higher level of policy feasibility and acceptance and a smoother operation accompanied with a higher effectiveness. For the two SARs, to achieve the objective to increase the recycling rate, the local Government must seek approval from China’s Central Government to allow locally generated recyclable material, in their original form, to enter mainland China for further treatment and to be turned into secondary raw material. Without such a proper support by a reverse resource logistic from the mainland, the CE schemes, such as the Mandatory Waste Source Separation, Producer Responsibility Scheme (PRS), Waste Charging Scheme, are not able to be implemented effectively. The current approach of the Local Government purchasing of recycling and exportation services of recyclable materials from private companies, and the sole reliance on existing market forces to handle, process, and export recyclable material out of the S.A.R.s cannot ensure a reliable and continuous operation in view of mitigating involved emissions. By way of a comparative analyses, the present investigation works out and distils suggestions for best practices of implementing the CE to comply with targets of emission reductions

  • Macao Creole Portuguese (MacCP) is a critically endangered language spoken in Southeastern China. The formation of MacCP is attributed to the speakers of Portuguese-based creole languages in Asia (Asian CPs), especially Papia Kristang, the Malayo-Portuguese of Malacca (MalCP). Since the 19th century, MacCP has been traditionally classified as Sino-Portuguese, but comparative methods incited some authors to treat MacCP within the Malayo-Portuguese group. In Macao, the Malaccan origin of MacCP, known as Patúa or Maquista, is generally underestimated or misunderstood by the local population, including the Macanese/Maquista community. The main goal of this research is to clarify the origin of MacCP from a typological perspective on grammatical features. Secondly, while considering a possible revitalization of Maquista, the research should assess the significance of the Malayo- and Sino-Portuguese classifications in popular narratives and relate the language to current practices. The grammar of MacCP emerged from the complex linguistic ecology of the Portuguese colonial expansion in Asia. The documentation of Asian CPs allows us to sketch possible scenarios that explain the formation of MacCP according to linguistic, historical and social factors. A digital corpus of MacCP containing archive documents, contemporary literature, and oral transcriptions was assembled in order to produce a systematic review of 130 grammatical features, as defined in the Atlas of Pidgin and Creole Language Structures Online (APiCS Online – Michaelis et al. [eds] 2013). MacCP and MalCP share certain features that are not found in South Asian CPs, such as the in situ position of interrogative words, the reduplication for nominal plural, the form of reciprocal constructions, and the verb serialization of motion constructions, thus pointing to the Malayo-Portuguese origin. At the same time, other features suggest a certain influence from Sinitic languages, mainly Cantonese and Hokkien, such as the convergence between the genitive, adjective and relative clause constructions, the double-object construction, the verb-neg-verb polar question, the copular focus construction, the reduplication inducing a change of word class or semantics, and the use of certain deontic, imperative, and prohibitive verbal markers. The comparative analysis of the grammars of MacCP, MalCP and other Asian CPs can be represented quantitatively by the means of a phylogenetic network (SplitsTree4 – Huson & Bryant 2006). The results clearly indicate that, from a structural perspective, MacCP belongs to the Malayo-Portuguese group and the presence of Sinitic elements did not affect the core of the grammar. In fact, MacCP and MalCP appear to be more similar to each other than to the former Malayo-Portuguese of Batavia. However, the Malayo-Portuguese classification of MacCP does not resonate with the Macanese community. By contrast, the Sino-Portuguese classification translates current linguistic, social and semiotic practices. A socio-semiotic survey among the millennial generation of Macanese and the consideration of themes and motifs in Maquista literature indicates that the revitalization of Maquista simultaneously implies, in their views, the preservation of the Cantonese and Portuguese heritage

  • Aggression clearly has an adaptive value as it is necessary to secure resources for survival, growth, and reproduction. The Siamese fighting fish, Betta splendens, have endured a prolonged process of artificial selection for winning paired-fight contests across Southeast Asia, resulting in strains of short-fin aggressive “fighters”. Across centuries, Thai breeders have been selecting these strains by discarding loser batches and allowing winner batches to breed, claiming that they are significantly more aggressive than wild-types. This natural experiment provides a powerful context to investigate the biological basis of aggressive behaviour in fish, the topic of this thesis. To study aggression, it is important to validate and standardize behavioural assays appropriate for the species under study. Further, different aggression-eliciting stimuli, such as live opponents, 3D models, video playback, or mirror images, may elicit non-equivalent behavioural and physiological responses. For B. splendens, in particular, quantifying aggression from live fights is not ethically acceptable as the high levels of aggression of this species usually result in injuries or even death of the opponent. In Chapter II, it was shown that mirror images elicit very similar aggressive displays and endocrine responses to an interacting opponent behind a transparent partition, validating the use of this test to measure aggression in this model species. Further, it was shown that circulating levels of both androgens (11-ketotestosterone and testosterone) and corticosteroids (cortisol) increased in response to the aggression challenge, even in the absence of conflict resolution, questioning the role of these hormones during present and future aggressive contests. Using the previously validated mirror assay and also tests with live conspecifics, we assessed the impact of selection for winning by comparing, in Chapter III, male and female aggressive behaviour of lab-raised fighter and wild-type strains. The hypothesis that selection for male winners enhanced aggressive displays was confirmed, suggesting that the duration and frequency of threat and attack behaviour correlates with winning probability. However, females of the fighter strain, which are not selected for fights, were also more aggressive than wild-type females. This suggests that male and female aggression share common genetic pathways and physiological mechanisms and raises the possibility that selection for alleles that favour male aggression may have promoted intersexual genetic conflict in this species. After confirming the expected differences in aggressive behaviour between fighter and wild-type fish, the following question was whether endocrine systems, in particular those previously shown to respond to aggression, could have been targeted by the selection process. From previous studies in fish and other vertebrates, it was hypothesised that selection for winners could have increased constitutive levels of androgens or led to an enhanced androgen response to a social challenge. However, in chapter IV, it was shown that levels of 11-ketotestosterone and its response to aggression was similar in males of both strains, questioning the role of androgens in the modulation of aggression in B. splendens. On the contrary, constitutive levels of cortisol and the response of this hormone to an aggression challenge were higher in wild-type compared with fighter fish, supporting previous findings that associated high aggression with a blunted cortisol response. Overall, results from Chapter IV suggest that selection for winning had a stronger impact on the hypothalamus-pituitary-interrenal axis than in the hypothalamus-pituitary-gonadal axis. My results support the assumption of the “Challenge Hypothesis” proposed by John Wingfield and collaborators in 1990 to explain the relationship between androgens and aggression, according to which androgen levels above a reproductive baseline are a consequence of the frequency and intensity of social interactions, in particular of male-male agonistic encounters. It is becoming clear that androgens increase rapidly after an aggressive contest, independently of fight outcome. However, the function of this increase remains unclear as the frequency of aggressive displays was unrelated with post-fight androgen levels and constitutive levels of androgens, and androgen responsiveness, were similar between fighter and wild-type males. Results obtained for cortisol agree with a “corticosteroid-mediated dominance hypothesis” whereby low baseline levels and a blunted response of corticosteroids would be associated with a dominant status and high aggression. The work advances our knowledge about the endocrine regulation of aggressive behaviour in B. splendens and opens several testable hypotheses about the role of androgens and corticosteroids in the regulation of fish aggressive behaviour

  • Mangroves are a unique group of plants, which offer a great variety of goods and services to the ecosystem and to the society. Regrettably, they have been globally threatened by urbanization and industrialization, among others, triggering overexploitation of the world’s mangrove forests despite their ecological and economic importance. As a result, mangroves are often under pollution stress as sinks or receivers for numerous man-made pollutants such as pesticides, which are the main focus of this thesis. One of the most widely applied chemicals in the word are the organochlorine pesticides (OCPs) that even after their supposedly worldwide ban between 1950s-1990s, they can still be detected in the environment. Numerous studies have been done in phytoremediation of pollutants by mangroves, but little attention has been given to the role of mangroves in the remediation of OCPs. For this reason, part of this thesis will focus on the occurrence and distribution of OCPs in intertidal tropical and sub-tropical areas around the world with and without mangroves. As a first goal (I), we evaluate —in a theoretical way— if the presence of mangroves affects or modifies the levels of OCPs in the surrounding environment. For this purpose, data from different matrices, such as water, sediment, benthic fauna and plants were included and discussed in this work. Moreover, and considering Macao’s location, we also quantified OCPs from surface waters of this region from areas with and without mangroves and included in this task. Besides this theoretical approach, this thesis also included some laboratoy and field work specifically focused on dicofol and 4,4’-dichlorobenzophenone (4,4’-DCBP, its main metabolite). Dicofol is an OCP strongly related to dichlorodiphenyltrichloroethane (DDT), which has been extensively used in China and more specifically, in the Pearl River Delta (PRD), a region under anthropogenic pressure. However, due to dicofol’s instability (i.e., sensitive to low pH, light exposure and high temperature), we expected to quantify 4,4′-DCBP (which is also common to DDT) as the main form present in the environment. As a second goal (II), we conducted a monitoring study in surface waters from Macao and Hong Kong, to evaluate the contamination status and water quality of these regions. Concentrations of 4,4’-DCBP, nutrients and physicochemical parameters were measured during transition and wet season, and at high and low tide. In addition, since the toxicity of this metabolite was totally unknown, we assessed it via two biological models: Daphnia magna and Artemia salina. Since 4,4’-DCBP was detected and quantified in both regions (2.8-30.0 ng/L), this thesis also includes experimental work focused on the assimilation and depuration pattern by a marine organism. For that purpose and as a final goal (III), we selected the common edible bivalve Meretrix as a model to evaluate the dynamics of accumulation and depuration of the pesticide dicofol. The Vietnamese clams were exposed during 15 days under two different concentrations of dicofol, and decontaminated for the same period of time. Quantification of 4,4’-DCBP was done during both phases (uptake and depuration) and at different sampling times. In summary, all these different works helped us to conclude that: I.1) As expected, vegetated areas with mangroves presented lower concentrations of OCPs for all the matrices, and also better quality in terms of pesticide pollution for water and sediments. Results obtained from Macao’s waters also revealed the same pattern, with mangroves areas having lower levels of contamination. Although the gathered data presented methodological variability (i.e. different quantification methods, extraction protocols, equipment used), the same pattern was observed among matrices, showing how robust and solid the results herein obtained are. II.1). Hong Kong presented higher concentrations of 4,4’-DCBP than Macao, which may be due to the use of dicofol as a pesticide and the use of antifouling-paint for ships. Moreover, concentrations of 4,4’-DCBP during wet season were below limits of quantification, demonstrating a seasonal pattern and a dilution effect due to higher river discharges during this period. II.2). Both regions showed possible eutrophication problems due to the high nutrient concentrations. These levels presented also a seasonal variability, with dissolved inorganic nitrogen and total dissolved solids higher during transition; and dissolved inorganic phosphorous, total suspended solids and chlorophyll a higher during wet season. II.3). Toxicity of 4,4’-DCBP was lower than the parent compound dicofol, and the levels quantified indicated a low environmental risk. However, it is important to pay attention to this compound since interaction with other contaminants could enhance their toxicity, or processes such as biomagnification or bioaccumulation could make low concentrations a threat for the environment. III.1). Different concentrations of dicofol presented different uptake and depuration kinetics. Animals exposed to higher concentrations (500 ng/L), had levels above limits of quantification (LOQ) after 24h exposure, unlike the ones exposed to lower concentrations (50 ng/L), which had levels <LOQ after the same period. The first ones also, presented lower uptake rates, and this could indicate that high dicofol concentrations in the system could affect the respiration rates of the organism. In addition, this work also showed that animals exposed to high concentrations of dicofol will need more than 15 days to depurate in order to reach safe levels for human consumption. The compilation of the work done in this thesis allowed us to better understand the role of mangroves ecosystems on the accumulation of OCPs and to provide solid information that could create strategies for mangroves management and conservation. Moreover, and as a first attempt, we were able to quantify this pesticide metabolite in the PRD (one of the most seriously contaminated areas in China), to determine its toxicity and to define its kinetics in an important organism such as the edible bivalve M. meretrix. We intend that this thesis will be helpful for the scientific community providing new insights regarding metabolite interactions (within and with other molecules) and toxicity (LC50 and theoretical risk assessment), which were unknown until now

  • Vehicles solely powered by electricity are a major technological innovation that combines individual transportation needs and environmental sustainability, yet their market penetration is low. Research has traditionally indicated factors such as the vehicle’s purchasing price, driving range, and charging time as the main barriers to adoption. However, the decision to adopt a technology also depends on what the technology represents to the user; therefore, other factors may be important to explain individuals’ behavior. This study is a quantitative and cross-sectional look at the behavioral intention to adopt battery electric vehicles (BEVs) technology in the context of Macau. The research builds on the unified theory of acceptance and use of technology 2 (UTAUT 2) (Venkatesh et. al., 2012) to explain the characteristics of the local consumers. Besides the addition of image and environmental concern to the theoretical model, the study also put forward and evaluate the construct of technology show-off, an original measure of the visible and experiential characteristics of a technology. A sample of 236 Macau residents was analyzed by structural equation modeling (SEM). The analysis of the data supported the explanatory and predictive power of our model and helped to describe the idiosyncrasies of local residents. The results provide insights related to individual technology acceptance that could be useful in designing more accurate strategies and fostering the uptake of BEVs in Macau or markets that share similarities

  • Fishes are highly specialized in extracting ecologically relevant information from their diverse acoustic habitats since early developmental stages. The zebrafish (Danio rerio) is a valuable and well-stablished vertebrate model for investigating hearing functioning and disorders, development of the inner ear in vertebrates including humans, drug discovery, ecotoxicology assessments and behavioral research. Although the acoustic environment is known to shape the structure and sensitivity of auditory systems, there is no information on the natural soundscape of this species. Zebrafish are typically reared in large-scale artificial housing systems, which acoustic properties and potential effects on hearing remain largely unknown. Even though elevated levels of noise are widely present in most aquatic soundscapes and to an even greater extent in artificial environments, very limited information is known on how this important environmental stressor impacts species’ development and physiology, hearing capabilities and inner ear morphology, and behaviour. Considering that noise pollution is rapidly increasing in aquatic ecosystems, causing detrimental effects on survivability and growth and altering physiology and behaviour of organisms, it is of paramount importance to assess how this stressor affects wildlife, especially in early ontogeny, a critical period for development and establishment of phenotypic traits. For this thesis I aimed to 1) characterize the soundscape of both zebrafish natural habitats and laboratory captive conditions, and discuss possible impact on auditory sensitivity. Sound recordings were conducted in five distinct zebrafish habitats (Southwest India), from quieter stagnant environments with diverse biological/abiotic sounds to louder watercourses characterized by current and moving substrate, while artificial environmental characterization was conducted on three typical zebrafish housing systems. 8 In order to assess the impact of noise exposure on early development, my next goal was to 2) perform a split-brood experiment to test the effects of chronic noise exposure to increasing levels (130 and 150 dB re 1 μPa, continuous white noise) and different temporal regimes (mimicking shipping activity) on larval zebrafish in regards to general development, physiological stress, and behavioural patterns. Finally, the last objective consisted on 3) testing the effects of chronic noise exposure on auditory sensitivity measured based on inner ear saccular microphonics and acoustic-evoked startle responses (prepulse inhibition paradigm) in larval zebrafish, as well as evaluating whether sensitivity changes were paralleled by altered inner ear morphology. Based on bioacoustics methods, my first study found that zebrafish natural soundscape varied between 98 and 126 dB re 1 lPa in sound pressure levels. Sound spectra presented most energy below 3000 Hz and quieter noise windows were found in the noisiest habitats matching the species best hearing range. Contrastingly, recordings from zebrafish housing systems revealed higher sound levels (122–143 dB) and most energy below 1000Hz with more spectral peaks, which might cause significant impact such as auditory masking or even hearing loss. In my second research work, the acoustic treatments did not affect general development or hatching but increased noise levels led to a significant increase in mortality of larval zebrafish. The cardiac rate, yolk sac consumption and cortisol levels increased significantly with increasing noise level at both 3 and 5 dpf (days post fertilization). Variations in noise time presentations (different random noise periods similar to shipping activity) suggested that the presence of longer silent intervals is important to down-regulate physiological stress. Moreover, 5 dpf larvae exposed to 150 dB continuous noise regimes displayed increased dark avoidance in an anxiety-related dark/light preference test and displayed a significant 9 impairment in spontaneous alternation behaviour (SAB) a memory and sensorimotor related behaviour. Finally, in the last thesis goal, I found that noise-exposed specimens displayed significantly lower hair cell number and saccular epithelial area. This change in sensory morphology was paralleled by a significant decrease in inner ear saccular sensitivity at lower frequencies (100 to 200 Hz) in 5 dpf larvae. Sensorimotor hearing assessment revealed a hypersensitisation effect in noise-exposed group that displayed higher startle swimming velocity, but also significant decrease in sensitivity at 200 Hz. Altogether, this thesis provides an important ground for future research on the adaptation of zebrafish auditory system to the natural soundscapes, and highlights the importance of controlling noise conditions in captivity systems. Furthermore, results provide first evidence of noise-induced physiological stress, anxiety-driven behaviours and memory impairment in larval zebrafish larvae, showing that both noise amplitude and timing may negatively impact key physiological and behavioural endpoints in early ontogeny. The thesis also reports new findings on how acoustic stress may impact the structure and function of the inner ear in larval fish, which was followed by decreased sensitivity in sensorimotor responses to acoustic stimuli. My research highlights the importance of investigating how altered soundscapes and associated physiological and behavioural stress may affect important sensitive windows in development and impose new evolutionary challenges under a scenario of global change

  • This thesis aims to explore how learning accounting for non-accounting major students could be enhanced through a comprehensive framework connecting individual attributes and learning approaches. While accounting is considered an important and mandatory discipline for business students, it is also considered a difficult subject which deters students’ engagement in the subject. Although the issue has been widely recognized, there are relatively less discussions on possible remedies. Learning accounting is a dynamic process that go beyond the traditional one-to-many learning and teaching settings, all factors revolving the students’ learning environment and process, altogether determine and affect the learning efficiencies. A meta-analysis was first performed to understand the standings of accounting education and to identify gaps within existing literatures in the field of management, accounting, tourism and general education researches. A documentary review on the curricula of all Macau HEI programs involving compulsory accounting courses was also conducted to understand the role and significance of accounting courses for non-accounting college students in Macau. Based on the literature review, this study borrows constructs from theory of planned behavior (TPB) models in its research framework. It attempts to provide a comprehensive framework to understand how students learn accounting efficiently by delving into individual learning styles of the students and their perceived helpfulness and self-efficacy, in respective to different learning techniques. Due to its exploratory nature, a mixed method of qualitative and quantitative methodology is adopted, where both focus group discussions and in-depth interviews are performed with non-accounting students to elicit understandings on student’s difficulties in learning accounting and their preferences for different learning styles. Then, a full-scale quantitative survey is conducted in a Macau higher education institute to assess the learning efficiencies of accounting in different situations. A triangulation with feedback from faculties and administrators are also elicited to provide alternate dimensions on the issues. With no surprise, there is no one best 3 teaching style or method for all students, this thesis contributes to accounting education research by provide a comprehensive diagnosis on the all-round learning stimulus and hindrance in learning accounting. At the end, recommendations are provided for accounting educators to emphasize on the uniqueness of students and to encourage a more dynamic and open environment for learning. Limitations of the studies are also discussed

Last update from database: 3/29/24, 8:07 AM (UTC)