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This current study assessed the toxicity of selected heavy metals in paddy and sediments of non-major production sites in Southern Peninsular Malaysia, complemented by bibliometric analysis of research trends and health implications of rice contamination. Paddy (grains, stems, roots) and soil samples were collected from seven selected sites in the Southern parts of Peninsular Malaysia and analyzed for their heavy metals content. The health risk assessments were conducted based on estimated daily intake, and the Web of Science database was used for bibliometric analysis. The results indicated elevated levels of manganese, Mn (0.4 ± 0.07), especially in the roots, compared to other heavy metals. Generally, the heavy metal levels in paddy grains were below FAO/WHO’s tolerable daily intake levels, indicating minimal non-carcinogenic risks to both adults and children. The bibliometric analysis indicated a significant increase in related publications, reflecting growing academic interest. This study highlights the potential of non-major sites to produce rice with lower contamination levels, provides insights into research trends, and identifies future investigation areas, especially for major production sites and post-COVID-19 periods. Therefore, this study offers a robust scientific context, identifies research gaps, benchmarks findings, and guides future research directions, ensuring an in-depth perception on heavy metal contamination and its health risks. © This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Adsorption of wine proteins is an essential step in the production of white and rosé wines. In order to develop environmentally friendly adsorption processes, non-swelling adsorbents are required. The performance of selected non-swelling ion-exchange resins (Macro-Prep™ 50S and Streamline® SP) was studied by describing the process kinetics of the adsorption of BSA in a model wine solution. The process was assumed to be diffusion controlled and a shrinking core model was applied. Experiments were performed in the 5–35°C temperature range and with different equilibrium partition coefficients. The results obtained with the shrinking core model were theoretically consistent and the apparent diffusivity values correlated very well with theoretically estimated effective diffusivities combined with a linear dependence of porosity with temperature. Separating the temperature effect on porosity, the apparent diffusivity followed an Arrhenius type dependency with temperature with 16.9 kJ/mole activation energy.

The protein adsorption capacity of different materials was evaluated to assess their potential efficacy as alternative adsorbents for the removal of proteins from wines, with the purpose of finding suitable low-swelling materials that could be used in a percolated bed. The adsorbents tested were thermally treated sodium bentonite, low-swelling adsorbing clays, ion exchange resins and other protein adsorbents (silica gel, hydroxyapatite and alumina). The materials were evaluated by analysing both the capacity to stabilize untreated white wines, according to a heat test, and by characterizing the adsorption isotherms of bovine serum albumin (BSA) in a model wine (water, ethanol and K-bitartrate). Breakthrough curves in a packed bed were determined experimentally for some materials, showing the influence of the adsorption isotherm shape. Some ion-exchange resins showed a favourable behaviour and have good potential as alternative adsorbents.

In Southeast Asia, males of the Siamese fighting fish Betta splendens have been selected across centuries for paired-staged fights. During the selection process, matched for size males fight in a small tank until the contest is resolved. Breeders discard losing batches and reproduce winner batches with the aim of increasing fight performance. We assessed the results of this long-term selection process by comparing under standard laboratory conditions male and female aggressive behaviour of one strain selected for staged fights (“fighters”) and one strain of wild-types. The aggressive response of adult fish was tested against their mirror image or a size-matched conspecific. Fighter males were more aggressive than wild-type males for all measured behaviours. Differences were not only quantitative but the pattern of fight display was also divergent. Fighter males had an overall higher swimming activity, performing frequent fast strikes in the direction of the intruder and displaying from a distance. Wild-type males were less active and exhibited aggressive displays mostly in close proximity to the stimuli. Females of the fighter strain, which are not used for fights, were also more aggressive than wild-type females. Aggressive behaviours were correlated across male and female fighter siblings, suggesting common genetic and physiological mechanisms to male and female aggression in this species. The study further shows that results were largely independent of the stimulus type, with the mirror test inducing similar and less variable responses than the live conspecific presentation. These results suggest that selection for male winners co-selected for high-frequency and metabolic demanding aggressive display in males and also enhanced female aggression, opening a wide range of testable hypothesis about the ultimate and proximate mechanisms of male and female aggression in B. splendens.

The role of hormones as modulators of aggressive behavior in fish remains poorly understood. Androgens and corticosteroids, in particular, have been associated with aggressive behavior in fish but it is still not clear if animals adjust the secretion of these hormones to regulate behavior during ongoing fights, in response to fight outcomes in order to adjust aggressive behavior in subsequent fights, or both. With its stereotyped displays and high aggression levels, the Siamese fighting fish Betta splendens is an excellent model to investigate this question. Here, we compared the behavioral and endocrine response of male B. splendens to fights where there is no winner or loser by presenting them with a size-matched live interacting conspecific behind a transparent partition or with a mirror image. The aggressive response started with threat displays that were overall similar in frequency and duration towards both types of stimuli. Fights transitioned to overt attacks and interacting with a live conspecific elicited a higher frequency of attempted bites and head hits, as compared with the mirror image. There was a pronounced increase in plasma androgens (11-ketotestosterone and testosterone) and corticosteroids (cortisol) levels in response to the aggression challenge, independent of stimulus type. Post-fight intra-group levels of these hormones did not correlate with measures of physical activity or aggressive behavior. A linear discriminant analysis including all behavioral and endocrine data was a poor classifier of fish from the conspecific and mirror trials, showing that overall the behavioral and endocrine response to mirror images and conspecifics was similar. The results show that fight resolution is not necessary to induce an evident increase in peripheral levels of androgens and corticosteroids in B. splendens. However, the function of these hormones during present and future aggressive contests remains to be clarified.

The occurrence of endocrine disrupting chemicals (EDCs) is a major issue for marine and coastal environments in the proximity of urban areas. The occurrence of EDCs in the Pearl River Delta region is well documented but specific data related to Macao is unavailable. The levels of bisphenol-A (BPA), estrone (E1), 17α-estradiol (αE2), 17β-estradiol (E2), estriol (E3), and 17α-ethynylestradiol (EE2) were measured in sediment samples collected along the coastline of Macao. BPA was found in all 45 collected samples with lower BPA concentrations associated to the presence of mangrove trees. Biodegradation assays were performed to evaluate the capacity of the microbial communities of the surveyed ecosystems to degrade BPA and its analogue BPS. Using sediments collected at a WWTP discharge point as inoculum, at a concentration of 2 mg l−1 complete removal of BPA was observed within 6 days, whereas for the same concentration BPS removal was of 95% after 10 days, which is particularly interesting since this compound is considered recalcitrant to biodegradation and likely to accumulate in the environment. Supplementation with BPA improved the degradation of bisphenol-S (BPS). Aiming at the isolation of EDCs-degrading bacteria, enrichments were established with sediments supplied with BPA, BPS, E2 and EE2, which led to the isolation of a bacterial strain, identified as Rhodoccoccus sp. ED55, able to degrade the four compounds at different extents. The isolated strain represents a valuable candidate for bioremediation of contaminated soils and waters.

The Dayingezhuang gold deposit in the Jiaodong district, eastern margin of the North China Craton is hosted in Mesozoic granitic rocks and consists of quartz-sulfide veins/veinlets and sulfide disseminations in alteration envelopes. Previous studies mainly focused on the geochronology, sources of ore-forming fluids and metals to investigate the ore genesis. However, enrichment mechanism of Au and other associated trace metals remain unclear. In this study, we present detailed textures and in-situ LA-ICP-MS trace-element compositions of different generations of pyrite, as well as EMP analysis of Au-bearing minerals to discuss the occurrence and enrichment mechanism of Au at this deposit. Three generations of pyrite (Py1, Py2 and Py3) formed during three hydrothermal ore stages (I, II, and III) at Dayingezhuang. Py1 occurs as disseminations in sericitic alteration assemblages and is characterized by low Au (mean 0.15 ppm), Ag, As and Te contents. The time-resolved depth-concentration profiles indicate that Au in Py1 mainly occurs as nanoparticles and/or micron-sized inclusions. Py2 can be further divided into the early undeformed Py2a and later Py2b, which is the product of deformed Py2a with different degrees of brittle to plastic deformation and recrystallization. Py2a in pyrite-siderite-quartz veins is relatively enriched in invisible Au (mean 0.41 ppm), Ag, As, and Te compared to Py1, and contains numerous micron-sized Au inclusions. In contrast, Py2b contains lesser invisible Au (0.21 ppm) and host abundant gold minerals along the grain boundaries and microfractures. Py3 in polymetallic sulfide veins has little Au. As a whole, Au in pyrite is positively correlated with Ag and Te, which is consistent with the results of EMP analysis showing the occurrence of Au as electrum, native gold and minor petzite in pyrite. Such evidences show that the deformation and recrystallization of auriferous Py2a potentially caused local remobilization of Au (mainly as micron-sized inclusion Au) via solid-state ductile flow and subsequent reconcentration of Au in microfractures of Py2b. The pyrite deformation and Au remobilization events were suggested to be related to the continuous reactivation of the regional Zhaoping Fault contemporaneous with gold mineralization. Our study highlights the importance of remobilization and reconcentration of Au triggered by syn-ore tectonic activities at Dayingezhuang and possibly other Au deposits in the Jiaodong district.

The prevalence of microplastics in the environment has become a major global conservation issue. One primary source of environmental microplastics is personal care and cosmetic products (PCCPs) containing microbeads. The market availability of PCCPs containing microbeads and the level of contamination of coastal sediments by microplastics was studied in one of the most densely populated cities in the world, Macao in China. We found that PCCPs containing microbeads are still widely available for sale in the region, with over 70% of surveyed PCCPs containing at least one type of microbeads as an ingredient, with polyethylene (PE) being the most common one. In an estimate, the use of PCCPs in the territory may release over 37 billion microbeads per year into the environment via wastewater treatment plants. The density of microplastics in coastal sediments varied between 259 and 1,743 items/L of sediment, amongst the highest reported in the world. The fraction of < 1 mm was the most abundant, representing an average of 98.6% of the total, and correlated positively with the abundance of larger sized fragments. The results show that although environmental pollution with microplastics released from PCCPs usage is significant, other sources, namely fragmentation of larger plastic debris, likely contribute more to the issue. The study highlights the magnitude of the problem at a local level and suggests possible mitigating strategies.

AimsVascular calcification is a common clinical complication of chronic kidney disease (CKD), atherosclerosis (AS), and diabetes, which is associated with increased cardiovascular morbidity and mortality in patients. The transdifferentiation of vascular smooth muscle cells (VSMCs) to an osteochondrogenic phenotype is a crucial step during vascular calcification. The transcription factor CCAAT/enhancer-binding protein alpha (C/EBPα) plays an important role in regulating cell proliferation and differentiation, but whether it regulates the calcification of arteries and VSMCs remains unclear. Therefore, this study aims to understand the role of C/EBPα in the regulation of vascular calcification.Methods and ResultsBoth mRNA and protein expression levels of C/EBPα were significantly increased in calcified arteries from mice treated with a high dose of vitamin D3 (vD3). Upregulation of C/EBPα was also observed in the high phosphate- and calcium-induced VSMC calcification process. The siRNA-mediated knockdown of C/EBPα significantly attenuated VSMC calcification in vitro. Moreover, C/EBPα depletion in VSMCs significantly reduced the mRNA expression of the osteochondrogenic genes, e.g., sex-determining region Y-box 9 (Sox9). C/EBPα overexpression can induce SOX9 overexpression. Similar changes in the protein expression of SOX9 were also observed in VSMCs after C/EBPα depletion or overexpression. In addition, silencing of Sox9 expression significantly inhibited the phosphate- and calcium-induced VSMC calcification in vitro.ConclusionFindings in this study indicate that C/EBPα is a key regulator of the osteochondrogenic transdifferentiation of VSMCs and vascular calcification, which may represent a novel therapeutic target for vascular calcification.

Limited special education and related services are available for children with autism spectrum disorder (ASD) in Macau, especially those who are educated in general education classrooms. No intervention study has been conducted on these children. This study was conducted to explore the relationship between a board game play intervention and board game play behaviors and social communication of children with ASD educated in general education classrooms in Macau. A repeated measures design was used and the results of this study showed the mean occurrence of unprompted board game play behaviors per session during intervention was not significantly different from that during pre- or post-intervention. The mean occurrence of social communication per session during intervention was significantly higher than that during pre- and post-intervention. These findings suggest a positive relationship existed between the board game intervention used in this study and social communication of children with ASD.

The key challenge of Unsupervised Domain Adaptation (UDA) for analyzing time series data is to learn domain-invariant representations by capturing complex temporal dependencies. In addition, existing unsupervised domain adaptation methods for time series data are designed to align marginal distribution between source and target domains. However, existing UDA methods (e.g. R-DANN Purushotham et al. (2017), VRADA Purushotham et al. (2017), CoDATS Wilson et al. (2020)) neglect the conditional distribution discrepancy between two domains, leading to misclassification of the target domain. Therefore, to learn domain-invariant representations by capturing the temporal dependencies and to reduce the conditional distribution discrepancy between two domains, a novel Attentive Recurrent Adversarial Domain Adaptation with Top-k time series pseudo-labeling method called ARADA-TK is proposed in this paper. In the experiments, our proposed method was compared with the state-of-the-art UDA methods (R-DANN, VRADA and CoDATS). Experimental results on four benchmark datasets revealed that ARADA-TK achieves superior classification accuracy when it is compared to the competing methods.

The Mesozoic gold deposits in the North China Craton (NCC) were hosted by the Precambrian basement and Mesozoic intrusions. Thus, most researchers consider that these gold deposits were genetically linked to the Mesozoic intrusions. However, we suggest that a metamorphic devolatilization model provides an alternative based on a combined Fe and in-situ S isotopes study on auriferous pyrites from the Baiyun gold deposit in the NCC. The Triassic Baiyun gold deposit contains the quartz vein and altered rock ores that were developed in the Paleoproterozoic metavolcanic-sedimentary rocks (the Liaohe Group). Our in-situ S isotopic analyses show that pyrites from the quartz vein ores are characterized by negative δ34S values (-10.7 ∼ -5.5‰), while those from the altered rock ores have two distinct groups of δ34S values, one being positive (+13.5 ∼ +16.2‰) and the other negative (-10.6 ∼ -3.0‰). We suggest that pyrite grains with positive δ34S values should be relicts from the host rocks, because they show comparable δ34S values with those from the host rocks schists (+3.3 ∼ +16.1‰). Thus, only the negative δ34S values of pyrites in ores (-10.7 ∼ -3.0‰) and the Fe isotopes of the quartz vein ores (δ56Fe = +0.30 ∼ +0.48‰) can represent the isotopic characteristics of ore-forming fluids at Baiyun. Our study shows that the sulfur were probably from the pyritic volcanic-sedimentary sequences of the Liaohe Group, rather than from magmas. The calculated δ56Fe values of the ore-forming fluids (-0.78 ∼ -0.37‰; pyrite-fluid isotope fractionation) could be modelled in a metamorphic devolatilization model with Fe-species (pyrite&magnetite) of the Liaohe Group as sources. Therefore, our combined S- and Fe- isotope data indicate that the metamorphic devolatilization of the Liaohe Group could account for the genesis of the Baiyun gold deposit.

In this paper, preliminary investigation was conducted to evaluate the potential ecological risk of heavy metals contamination in cemetery soils. Necrosol samples were collected from within and around the vicinity of the largest mass grave in Rwanda and analyzed for heavy metal concentrations using total digestion–inductively coupled plasma mass spectrometry and instrumental neutron activation analysis. Based on the concentrations of As, Cu, Cr, Pb, and Zn, the overall contamination degree (Cdeg) and potential ecological risks status (RI) of the necrosols were determined. The preliminary results revealed that the associated cemetery soils are only contaminated to a low degree. On the other hand, assessment of the potential ecological risk index (RI) revealed that cumulative heavy metal content of the soil do not pose any significant ecological risks. These findings, therefore, suggest that, while cemetery soils may be toxic due to the accumulation of certain heavy metals, their overall ecological risks may be minimal and insignificant.

In southeast Asia, males of the Siamese fighting fish, Betta splendens, have been selected across centuries for winning paired staged fights and previous work has shown that males from fighter strains are more aggressive than wild-types. This strong directional selection for winners is likely to have targeted aggression-related endocrine systems, and a comparison between fighter and wild-type strains can bring into evidence the key hormones implicated in aggression. Here, we compared the plasma levels of the androgen 11-ketotestosterone (KT) and of the corticosteroid cortisol (F) in F2 males of a fighter and a wild-type strain raised under similar laboratory conditions. We show that F was generally lower in fighter as compared with wild-type males, while no overall differences in KT levels were detected between strains. When presented with a mirror-induced aggressive challenge, post-fight levels of F increased but more significantly so in wild-type males, while KT increased in males of both strains. After the challenge, fighter males had higher levels of KT as compared with wild-type males, while the pattern for F was opposite. As compared with animals in social groups, wild-type males placed under social isolation had lower F levels, while KT decreased for fighters. Taken together, this data suggests that while wild-type males responded to aggression with an increase in circulating levels of both androgens and corticosteroids, males selected for winning fights maintained a blunt F response, increasing only KT levels. These data agree with the hypothesis that a combination of high levels of androgens and low levels of corticosteroids is associated with high aggression. Overall, these results seem to indicate that selection for winning had a stronger impact in the hypothalamus-pituitary-interrenal axis than in the hypothalamus-pituitary–gonadal axis in B. splendens.

Parental nutrient reserves are directly related to reproductive performance in sea cucumbers. This study focused on the lipid requirements of male and female sea cucumbers Apostichopus japonicus during the reproductive stage and analyzed their physiological responses to a high-fat diet (HFD). The intestinal lipid metabolites and microbiome profile changed significantly in animals fed with the HFD, as given by an upregulation of metabolites related to lipid metabolism and an increase in the predominance of Proteobacteria in the microbiome, respectively. The metabolic responses of male and female sea cucumbers to the HFD differed, which in turn could have triggered sex-related differences in the intestinal microbiome. These results suggest that the lipid content in diets can be differentially adjusted for male and female sea cucumbers to improve nutrition and promote reproduction. This data contributes to a better understanding of the reproductive biology and sex differences of sea cucumbers.

Air pollution in Macau has become a serious problem following the Pearl River Delta’s (PRD) rapid industrialization that began in the 1990s. With this in mind, Macau needs an air quality forecast system that accurately predicts pollutant concentration during the occurrence of pollution episodes to warn the public ahead of time. Five different state-of-the-art machine learning (ML) algorithms were applied to create predictive models to forecast PM2.5, PM10, and CO concentrations for the next 24 and 48 h, which included artificial neural networks (ANN), random forest (RF), extreme gradient boosting (XGBoost), support vector machine (SVM), and multiple linear regression (MLR), to determine the best ML algorithms for the respective pollutants and time scale. The diurnal measurements of air quality data in Macau from 2016 to 2021 were obtained for this work. The 2020 and 2021 datasets were used for model testing, while the four-year data before 2020 and 2021 were used to build and train the ML models. Results show that the ANN, RF, XGBoost, SVM, and MLR models were able to provide good performance in building up a 24-h forecast with a higher coefficient of determination (R2) and lower root mean square error (RMSE), mean absolute error (MAE), and biases (BIAS). Meanwhile, all the ML models in the 48-h forecasting performance were satisfactory enough to be accepted as a two-day continuous forecast even if the R2 value was lower than the 24-h forecast. The 48-h forecasting model could be further improved by proper feature selection based on the 24-h dataset, using the Shapley Additive Explanations (SHAP) value test and the adjusted R2 value of the 48-h forecasting model. In conclusion, the above five ML algorithms were able to successfully forecast the 24 and 48 h of pollutant concentration in Macau, with the RF and SVM models performing the best in the prediction of PM2.5 and PM10, and CO in both 24 and 48-h forecasts.

Employees work long hours in an environment where the ambient air quality is poor, directly affecting their work efficiency. The concentration of particulate matters (PM) produced by the interior renovation of shopping malls has not received particular attention in Macao. Therefore, this study will investigate the indoor air quality (IAQ), in particular of PM2.5, in large-scale shopping mall renovation projects. This study collected on-site PM data with low-cost portable monitoring equipment placed temporarily at specific locations to examine whether the current control measures are appropriate and propose some improvements. Prior to this study, there were no measures being implemented, and on-site monitoring to assess the levels of PM2.5 concentrations was non-existent. The results show the highest level of PM2.5 recorded in this study was 559.00 μg/m3. Moreover, this study may provide a reference for decision-makers, management, construction teams, design consultant teams, and renovation teams of large-scale projects. In addition, the monitoring of IAQ can ensure a comfortable environment for employees and customers. This study concluded that the levels of PM2.5 concentration have no correlation with the number of on-site workers, but rather were largely influenced by the processes being performed on-site.