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Uptake and depuration kinetics of 4,4′-dichlorobenzophenone (main metabolite of dicofol) in the edible clam Meretrix meretrix were evaluated through a mesocosm experiment. M. meretrix was exposed to different dicofol concentrations (environmental concentration, D1 = 50 ng/L; supra-environmental concentration, D2 = 500 ng/L) for 15 days, followed by the same depuration period. To accomplish this goal, an analytical method was successfully optimized for 4,4′-DCBP using QuEChERS as extraction method with a range of concentrations 0.3–76.8 ng/g ww quantified by gas chromatography coupled to tandem mass spectrometry. Our results demonstrated different kinetics of accumulation depending on the two dicofol treatments. For D1, the uptake kinetic was best fitted using a plateau followed by one phase association kinetic model, while for D2 a one phase association kinetic model suited better. Similar bioconcentration factors were obtained for both concentrations but only animals exposed to D2, showed 4,4′-DCBP levels above the limits of quantification after 24 h exposure. These animals also showed lower uptake rate (ku) than organisms exposed to D1. During the depuration period, only organisms exposed to D1 successfully depurated after 24 h. On the other hand, although animals exposed to D2 presented higher elimination factor, they did not reach the original levels after depuration. Moreover, values detected in these clams were higher than the Maximum Residue Level (10 ng/g) established by the European legislation. This indicates that longer periods of depuration time than the ones used in this study, may be needed in order to reach safe levels for human consumption. This work also demonstrated that studies on metabolite kinetics during uptake/depuration experiments, could be a new alternative to understand the impact and metabolism of pesticides in the marine environment.

The extraction of 21 insecticides and 5 metabolites was performed using an optimized and validated QuEChERS protocol that was further used for the quantification (GC–MS/MS) in several seafood matrices (crustaceans, bivalves, and fish-mudskippers). Seven species, acquired from Hong Kong and Macao wet markets (a region so far poorly monitored), were selected based on their commercial importance in the Indo-Pacific region, market abundance, and affordable price. Among them, mussels from Hong Kong, together with mudskippers from Macao, presented the highest insecticide concentrations (median values of 30.33 and 23.90 ng/g WW, respectively). Residual levels of fenobucarb, DDTs, HCHs, and heptachlors were above the established threshold (10 ng/g WW) for human consumption according to the European and Chinese legislations: for example, in fish-mudskippers, DDTs, fenobucarb, and heptachlors (5-, 20- and tenfold, respectively), and in bivalves, HCHs (fourfold) had higher levels than the threshold. Risk assessment revealed potential human health effects (e.g., neurotoxicity), especially through fish and bivalve consumption (non-carcinogenic risk; ΣHQLT > 1), and a potential concern of lifetime cancer risk development through the consumption of fish, bivalves, and crustaceans collected from these markets (carcinogenic risk; ΣTCR > 10–4). Since these results indicate polluted regions, where the seafood is collected/produced, a strict monitoring framework should be implemented in those areas to improve food quality and safety of seafood products.

Environmental education (EE) has long been practiced worldwide, while Nature-based solutions (NBS) is a relatively new concept. This chapter aims to provide an overview of the EE and NBS practices in East Asia and evaluate how these two valuable applications can be used concurrently. East Asia has a well developed environmental education (EE) programs and activities, both in formal and informal education. These ranges from developing green schools and campuses to establishing policies and acts. While EE has been actively practiced for decades in the region, the adoption of NBS to address environmental and societal challenges is limited. The educational benefits and opportunities from NBS are also lacking. Although there are some projects that can be classified as NBS, like the use of wetlands for wastewater treatment, they are not clearly categorized as one. These projects are also not integrated into environmental education programs. Considering this, the region should develop innovative environmental education programs for schools, universities and communities, that integrate NBS projects. Integrating the two together will boost the effectiveness of environmental education in raising environmental awareness and changing the environmental attitude and behavior of people, which will also help address societal issues.

Mangrove forests are one of the most ecologically valuable ecosystems in the world and provide a wide variety of ecosystem services to coastal communities, including cities. Macao, a highly urbanized coastal city located on the southern coast of China west of the Pearl River, is home to several species of mangroves with many associated flora and fauna. Mangrove forests in Macao are vulnerable to threats due to pressure from rapid and massive urban developments in the area, which led to mangrove loss in the past decades. To address this issue, the local authorities established special Ecological Zones for the management of the local mangroves. To reinforce local conservation efforts, educating the local population about the value of mangroves, especially school students, is of utmost importance. To evaluate the impact of environmental education activities on the environmental orientation, knowledge, and values of students toward mangrove conservation in Macao, a quasi-experimental study was undertaken. The effectiveness of a mangroves exhibition and field visit were evaluated using the New Environmental Paradigm (NEP) Scale—Macao version in a group of local school students who participated in the activities. Overall, the results provided consistently positive evaluations of the impact of the environmental education program. The strongest improvements were found in the students’ pro-environmental orientations, knowledge about mangroves, and value for environmental protection.

Genotoxic effects of dicofol on the edible clam Meretrix meretrix were investigated through a mesocosm experiment. Individuals of M. meretrix, were exposed to environmental concentration (D1 = 50 ng/L) and supra-environmental concentration (D2 = 500 ng/L) of dicofol for 15 days, followed by the same depuration period. DNA damage (i.e., strand breaks and alkali-labile sites) was evaluated at day 1, 7 and 15, during uptake and depuration, using Comet assay (alkaline version) and nuclear abnormalities (NAs) as genotoxicity biomarkers. The protective effects of dicofol against DNA damage induced by ex vivo hydrogen peroxide (H2O2) exposure were also assessed. Comet assay results revealed no significant DNA damages under dicofol exposure, indicating 1) apparent lack of genotoxicity of dicofol to the tested conditions and/or 2) resistance of the animals due to optimal adaptation to stress conditions. Moreover, ex vivo H2O2 exposure showed an increase in the DNA damage in all the treatments without significant differences between them. However, considering only the DNA damage induced by H2O2 during uptake phase, D1 animals had significantly lower DNA damage than those from other treatments, revealing higher protection against a second stressor. NAs data showed a decrease in the % of cells with polymorphic, kidney shape, notched or lobbed nucleus, along the experiment. The combination of these results supports the idea that the clams used in the experiment were probably collected from a stressful environment (in this case Pearl River Delta region) which could have triggered some degree of adaptation to those environmental conditions, explaining the lack of DNA damages and highlighting the importance of organisms’ origin and the conditions that they were exposed during their lives.

Mangroves are a unique group of plants growing along tropical and sub-tropical coastlines, with the ability to remove several types of contaminants such as heavy metals and other persistent organic compounds in coastal waters. However, little attention has been given to the possible role of mangroves in the removal of organochlorinated pesticides (OCPs) from the environment. Used worldwide, these pesticides were banned in the late 80s, withal they can still be quantified in aquatic environments due to their high stability. Moreover, as persistent and lipophilic compounds, OCPs are known for their tendency to bioaccumulate and biomagnify through the food chain, affecting local ecosystems, and potentially human health. This work aimed to investigate the potential benefits of mangrove ecosystems as OCP phytoremediators. For this purpose, a total of seventy-three articles from non-mangrove and mangrove areas around the world were gathered, integrated and re-analysed as a whole. These data include information from four different matrices (water, sediment, benthic fauna and mangrove plants). A common trend of less pesticide contamination in mangrove areas was observed for all the selected matrices. As a complement, average concentrations were discussed considering International Directives, such as the European legislation 2013/39/EU for water policy and the Dutch List together with the International Sediment Quality Guideline, for sediments. Additionally, theoretical risk assessments were also included. Since information regarding OCPs in mangroves ecosystem is very scarce compared to non-mangrove areas, this review provides valuable insights regarding these environments, and the importance of preserving them as a relevant remediation unit.