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This study aims to investigate gender-differentiated parenting and the factors that affect parent-adolescent relationships in Macao families. The study will address the following research questions: A) Does gender-differentiated parenting exist in Macao families? B) How does the academic level of parents relate to conflict resolution and acceptance levels? C) How does the length of time parents have lived together relate to conflict resolution and acceptance levels? D) How does the sibling position of adolescents relate to conflict resolution and acceptance levels? The study employed a quantitative research approach with a purposive and convenient sample of parents with children aged between 12 to 18 years old in Macao. The data were collected using the Parent-Child Interaction Questionnaire—Revised-Parent version (PACHIQ-R-P) through a questionnaire survey from May to August 2022. Out of the 172 completed questionnaires, seven were invalid, resulting in 165 valid questionnaires. The findings showed that gender differentiation in parenting was confirmed, with significant results showing that fathers had lower acceptance and conflict resolution levels when their child was a boy. Mothers showed similar acceptance and conflict resolution levels regardless of their child's gender. Furthermore, the study found that parents' acceptance of their children was influenced by their academic level, length of time living together, and the child's sibling position. This study is the first of its kind in Macao and could provide valuable insights for family and adolescent services in the region