TY - CHAP TI - Development of Structure and Sensitivity of the Fish Inner Ear AU - Vasconcelos, Raquel O. AU - Alderks, Peter W. AU - Sisneros, Joseph A. T2 - Fish Hearing and Bioacoustics: An Anthology in Honor of Arthur N. Popper and Richard R. Fay A2 - Sisneros, Joseph A. T3 - Advances in Experimental Medicine and Biology AB - Fish represent the largest group of vertebrates and display the greatest diversity of auditory structures. However, studies addressing how the form and function of the auditory system change during development to enhance perception of the acoustic environment are rather sparse in this taxon compared to other vertebrate groups. An ontogenetic perspective of the auditory system in fishes provides a readily testable framework for understanding structure–function relationships. Additionally, studying ancestral models such as fish can convey valuable comparable information across vertebrates, as early developmental events are often evolutionary conserved. This chapter reviews the literature on the morphological development of the fish auditory system, with particular focus on the inner ear structures that evolve from an otic placode during early embryonic development and then continue to undergo differentiation and maturation in the postembryonic phase. Moreover, the chapter provides a systematic overview of how auditory sensitivity develops during ontogeny. Although most studies indicate a developmental improvement in auditory sensitivity, there is considerably species-specific variation. Lastly, the paucity of information and literature concerning the development of auditory capabilities for social communication in fishes is also discussed. Further investigation on the development of structure and function of the fish auditory system is recommended in order to obtain a deeper understanding of how ontogenetic morphological changes in the auditory pathway relate to modifications in acoustic reception, auditory processing, and the capacity to communicate acoustically. CY - Cham DA - 2016/// PY - 2016 DP - Springer Link SP - 291 EP - 318 LA - en PB - Springer International Publishing SN - 978-3-319-21059-9 UR - https://doi.org/10.1007/978-3-319-21059-9_14 Y2 - 2021/02/10/09:06:28 KW - Auditory development KW - Auditory physiology KW - Ear morphology KW - Fish ontogeny ER - TY - JOUR TI - Vocal differentiation parallels development of auditory saccular sensitivity in a highly soniferous fish AU - Vasconcelos, Raquel O. AU - Alderks, Peter W. AU - Ramos, Andreia AU - Fonseca, Paulo J. AU - Amorim, M. Clara P. AU - Sisneros, Joseph A. T2 - Journal of Experimental Biology AB - Vocal differentiation is widely documented in birds and mammals but has been poorly investigated in other vertebrates, including fish, which represent the oldest extant vertebrate group. Neural circuitry controlling vocal behaviour is thought to have evolved from conserved brain areas that originated in fish, making this taxon key to understanding the evolution and development of the vertebrate vocal-auditory systems. This study examines ontogenetic changes in the vocal repertoire and whether vocal differentiation parallels auditory development in the Lusitanian toadfish Halobatrachus didactylus (Batrachoididae). This species exhibits a complex acoustic repertoire and is vocally active during early development. Vocalisations were recorded during social interactions for four size groups (fry: <2 cm; small juveniles: 2–4 cm; large juveniles: 5–7 cm; adults >25 cm, standard length). Auditory sensitivity of juveniles and adults was determined based on evoked potentials recorded from the inner ear saccule in response to pure tones of 75–945 Hz. We show an ontogenetic increment in the vocal repertoire from simple broadband-pulsed ‘grunts’ that later differentiate into four distinct vocalisations, including low-frequency amplitude-modulated ‘boatwhistles’. Whereas fry emitted mostly single grunts, large juveniles exhibited vocalisations similar to the adult vocal repertoire. Saccular sensitivity revealed a three-fold enhancement at most frequencies tested from small to large juveniles; however, large juveniles were similar in sensitivity to adults. We provide the first clear evidence of ontogenetic vocal differentiation in fish, as previously described for higher vertebrates. Our results suggest a parallel development between the vocal motor pathway and the peripheral auditory system for acoustic social communication in fish. DA - 2015/09/01/ PY - 2015 DO - 10.1242/jeb.123059 DP - Silverchair VL - 218 IS - 18 SP - 2864 EP - 2872 J2 - Journal of Experimental Biology SN - 0022-0949 UR - https://doi.org/10.1242/jeb.123059 Y2 - 2022/09/21/08:17:19 ER - TY - RPRT TI - Listening to the Environment: Importance of Early Acoustic Experience on Hearing and Development in Zebrafish AU - Vasconcelos, Raquel CY - Macao DA - 2016/// PY - 2016 PB - University of Saint Joseph ER - TY - RPRT TI - Listening to the Environment: Importance of Early Acoustic Experience on Hearing and Development in Zebrafish AU - Vasconcelos, Raquel CY - Macao DA - 2018/// PY - 2018 M3 - Research Report PB - University of Saint Joseph ER - TY - RPRT TI - The vocal brain of fish: development of auditory processing and vocal motor control AU - Vasconcelos, Raquel CY - Macao DA - 2016/// PY - 2016 LA - English M3 - Research Report PB - University of Saint Joseph ER - TY - RPRT TI - Bringing Life Science to Primary and Secondary School Students in Macao: From Genes to Environment AU - Tagulao, Karen CY - Macao DA - 2015/// PY - 2015 M3 - Research Report PB - University of Saint Joseph ER - TY - JOUR TI - Interpopulational variation of the mating system in the peacock blenny Salaria pavo AU - Saraiva, João L. AU - Pignolo, Giulia AU - Gonçalves, David AU - Oliveira, Rui F. T2 - acta ethologica DA - 2012/04// PY - 2012 DO - 10.1007/s10211-011-0104-y DP - DOI.org (Crossref) VL - 15 IS - 1 SP - 25 EP - 31 J2 - acta ethol LA - en SN - 0873-9749, 1437-9546 UR - http://link.springer.com/10.1007/s10211-011-0104-y Y2 - 2021/02/19/09:00:17 ER - TY - JOUR TI - Ecological modulation of reproductive behaviour in the peacock blenny: a mini-review AU - Saraiva, J. L. AU - Gonçalves, David AU - Oliveira, R. F. T2 - Fish Physiology and Biochemistry DA - 2013/02// PY - 2013 DO - 10.1007/s10695-012-9658-5 VL - 39 IS - 1 SP - 85 EP - 89 J2 - Fish Physiol Biochem LA - en SN - 0920-1742, 1573-5168 ST - Ecological modulation of reproductive behaviour in the peacock blenny UR - http://link.springer.com/10.1007/s10695-012-9658-5 Y2 - 2021/02/19/08:52:59 ER - TY - JOUR TI - Artificial selection for male winners in the Siamese fighting fish Betta splendens correlates with high female aggression AU - Ramos, A. AU - Gonçalves, D. T2 - Frontiers in Zoology AB - In Southeast Asia, males of the Siamese fighting fish Betta splendens have been selected across centuries for paired-staged fights. During the selection process, matched for size males fight in a small tank until the contest is resolved. Breeders discard losing batches and reproduce winner batches with the aim of increasing fight performance. We assessed the results of this long-term selection process by comparing under standard laboratory conditions male and female aggressive behaviour of one strain selected for staged fights (“fighters”) and one strain of wild-types. The aggressive response of adult fish was tested against their mirror image or a size-matched conspecific. Fighter males were more aggressive than wild-type males for all measured behaviours. Differences were not only quantitative but the pattern of fight display was also divergent. Fighter males had an overall higher swimming activity, performing frequent fast strikes in the direction of the intruder and displaying from a distance. Wild-type males were less active and exhibited aggressive displays mostly in close proximity to the stimuli. Females of the fighter strain, which are not used for fights, were also more aggressive than wild-type females. Aggressive behaviours were correlated across male and female fighter siblings, suggesting common genetic and physiological mechanisms to male and female aggression in this species. The study further shows that results were largely independent of the stimulus type, with the mirror test inducing similar and less variable responses than the live conspecific presentation. These results suggest that selection for male winners co-selected for high-frequency and metabolic demanding aggressive display in males and also enhanced female aggression, opening a wide range of testable hypothesis about the ultimate and proximate mechanisms of male and female aggression in B. splendens. DA - 2019/08/08/ PY - 2019 DO - 10.1186/s12983-019-0333-x DP - BioMed Central VL - 16 IS - 1 SP - 34 J2 - Frontiers in Zoology SN - 1742-9994 UR - https://doi.org/10.1186/s12983-019-0333-x Y2 - 2023/04/11/14:17:52 KW - Aggression KW - Artificial selection KW - Domestication KW - Mirror test KW - Sexual conflict ER - TY - THES TI - Petrology and geochemistry of the igneous rocks from Macao (South China) :tectono-magmatic implications AU - Quelhas, Pedro Moniz A3 - Dias, Ágata A3 - Mata, João AB - The territory of Macao is composed of several granitic intrusions belonging to one of the biggest granite provinces in the world, the Southeast China Magmatic Belt (SCMB), located in the southeast (SE) area of the Cathaysia Block. The SCMB is known by the occurrence of large volumes of Mesozoic magmatic rocks (over 90% are granitic rocks and equivalent volcanic rocks with minor basalts), occupying a total outcrop area of nearly 200.000 km2. The geology of Macao (~30 Km2) is dominated by granitic rocks displaying a wide range of textural, mineralogical and chemical features, making it an ideal region to study these rocks and the petrogenetic processes responsible for their diversity. This study employed a wide range of research methodologies, namely field studies, petrography, zircon geochronology, mineral chemistry, whole-rock elemental and isotopic geochemistry to determine the nature of the source, the petrogenetic processes and the tectonic regimes of the Mesozoic magmatism in this region. Thus, the data collected along this study aims to provide new knowledge on the tectono-magmatic evolution of Macao, in particular, and of SE China, in general. The results obtained from the high-precision U–Pb zircon geochronology, acquired through isotope dilution thermal ionization mass spectrometry (ID-TIMS) and in-situ laser ablation multi-collector inductively coupled mass spectrometry (LA-MC-ICPMS), revealed different ages for Macao granites. Despite its relatively small area, the determined ages tightly constrain the Macao granitic magmatism to two periods, ranging from 164.5 ± 0.6 to 162.9 ± 0.7 Ma (MGI – Macao Group I granites) and 156.6 ± 0.2 to 155.5 ± 0.8 Ma (MGII – Macao Group II granites). In addition, younger dacitic rocks were dated at 150.6 ± 0.6 Ma and <120 Ma. The existence of two proximal but distinct granitic pulses, spanning for a time of about 9 Ma and separated by ca. 6 Ma, in the Macao granitic suite suggests that it was incrementally assembled. This hypothesis is also extendable to the neighbouring Hong Kong region, where the magmatic activity occurred in four major pulses spanning for about 24 Ma. However, the MGII granites indicate the occurrence, on the Pearl River Delta region, of a magmatic pulse between those defined in Hong Kong at the origin of Lamma Suite (165–160 Ma) and the Kwai Chung Suite (148–146 Ma). In addition, Rare Element Earth (REE) ratios suggest that this pulse may only occur in Macao area, while MGI granites show evolving trends of REE ratios similar to those of Jurassic granites outcropping in vast areas of the Cathaysia Block (SE China). Inheritance patterns in the zircon U–Pb data indicate the presence of a population of antecrysts (165–180 Ma) crystallized from earlier magmatic pulses and a population of inherited zircons, from Precambrian to Phanerozoic sources, incorporated into the magmas during melting and/or ascent/emplacement at crustal levels. The oldest inherited ages (2.4 Ga and possibly 2.9 Ga) suggest contribution of Proterozoic and possibly of late Archaean crustal sources for the Macao magmatism. The granitic rocks of Macao are mainly high-K calc-alkaline metaluminous to weakly peraluminous I-type granites with variable degrees of fractionation. Fractional crystallization played an important role in the evolution of these granites, though the fractionation paths differ for the highly fractionated facies of both groups mainly due to distinct accessory fractionating phases. Such difference is evident by distinct REE evolution trends: while MGI magmas seem to have evolved by gradual enrichment in heavy REE relatively to light REE, originating progressively flatter REE patterns, magmas from MGII are marked by depletion of middle REE, leading to progressively concave upward REE patterns. However, while most of the geochemical variation of the MGII granites can be explained by fractional crystallization, the same is not true for MGI granites. The MGI highly fractionated granites show evidence for the REE tetrad effect and are characterized by non-CHArge-and-RAdius-Controlled (non-CHARAC) behaviour of trace elements, suggesting late-stage melt/fluid interactions involving F-rich fluids. The stage of evolution represented by the MGI highly fractionated granites corresponds to the onset of fluid/melt interaction in a highly evolved granitic system, which may have led to enhanced hydrothermal activity in more evolved stages, as those represented in neighbouring areas in SE China. Significant differences in isotopic composition were also observed, with the MGII being characterized by a much narrow range of initial 87Sr/86Sr ratios and εNd(t) and εHf(t) values than MGI. Based on these differences, the MGII granites are considered to be part of a comagmatic suite that has evolved in closed system, contrasting with what can be inferred for MGI. The increase in initial 87Sr/86Sr ratios with degree of evolution, the presence of metasedimentary enclaves and the high percentage of inherited zircon with Paleozoic ages in MGI, suggest the occurrence of an assimilation fractionation crystallization (AFC) process. The AFC processes were a major cause for the I-S transitional characteristics of the MGI highly fractionated granites and possibly of the other similar Jurassic biotite granites in SE China. The observed decoupling of Sr, Nd and Hf isotope systems might have resulted from magma mixing between the granitic and more mafic magmas, which caused the homogenization of Sr isotope ratios but not of the Nd and Hf ones. Such process is also supported by the occurrence of Microgranular Mafic Enclaves (MME) hosted by the granites. Isotope and major element compositions together with model ages strongly suggest that Macao granitic magmas were generated by partial melting of infracrustal medium-to-high K basaltic Paleo-Proterozoic to Mesoproterozoic protoliths heated by, and mixed to some degree with mantle-derived magmas. The temporal and spatial association of Macao and SE China Jurassic I-type granites with basaltic/gabbroic rocks, syenites and A-type granites, displaying typical intraplate chemical features, indicates an extensional regime rather than an active margin for the origin of these rocks. It also points to an important role of mantle-derived magmas in the production of SE China Jurassic granites. Adiabatic decompression melting of the asthenospheric mantle produced mafic magmas, which underplated at the base of the crust (Moho), subsequently triggering partial melting of the lower crust to generate the granitic magmas. The Jurassic Macao granites are interpreted as being produced in an intraplate extensional setting related to the break-off and foundering of a previously flat-slab (Paleo-Pacific plate) beneath the Eurasian plate. The boundary between east and west Cathaysia blocks is roughly along the Zhenghe-Dapu Fault (ZDF), which intersects the SE China coast near Hong Kong and is thought to have played a major role for the Mesozoic magmatic activity in this region. The stronger isotopic affinities of Macao granites with the other granitic rocks and lower crustal xenoliths from the western Cathaysia Block suggest that the ZDF is likely to pass south of Macao, a fact that has not been mentioned before. In addition to the Early Yanshanian (Jurassic) granitic magmatism in Macao, the younger ages obtained for the dacite dykes indicate that the territory was also affected, to a lesser degree, by Late Yanshanian (Cretaceous) magmatism. The transition from granitic to dacitic magmatism most likely corresponds to a change in the regional tectonic regime, which induced a significant change in the magma genesis processes. In contrast with the intraplate features of Macao and SE China Jurassic granites and coeval mafic rocks, the geochemical features of the Macao dacite dykes (e.g. high LILE/HFSE ratios and negative anomalies of Nb, Ta and Ti) are identical to those characterizing arc-like subduction-related magmas. These dacites are most likely evolved products of arc-like magmatism and may testify the reestablishment of a normal subduction system in this area of SE China. CN - D-SC 2019 QUE,PED CY - Macao DA - 2019/// PY - 2019 DP - USJ Library SP - 400 LA - English M3 - PhD in Science PB - University of Saint Joseph; Universidade de Lisboa. Faculdade de Ciências ST - Petrology and geochemistry of the igneous rocks from Macao (South China) UR - http://library-opac.usj.edu.mo/cgi-bin/koha/opac-detail.pl?biblionumber=173252 KW - Thesis and Dissertations PhD in Science (D-SC) KW - University of Saint Joseph ER - TY - JOUR TI - Mesozoic Granitic Magmatism in Macao, Southeast China AU - Quelhas, P. M. AU - Mata, J. AU - Lou, U. T. AU - Ribeiro, M. L. AU - Dias, Ágata T2 - AGU Fall Meeting Abstracts AB - Macao ( 30 Km2) is a territory characterized by small granitic intrusions, located along the coastal region of Southeast China (Cathaysia Block). Granitoids occur as different facies, including microgranite dykes, with distinct textural, mineralogical and geochemical features, for which a middle-upper Jurassic age ( 164 Ma) has been proposed. New data suggest that these granitoids are mostly high-K calc-alkaline metaluminous (A/CNK = 0.8 - 1.1) biotite granites, consistent with total absence of primary muscovite. They show variable amounts of SiO2 (67-77%), reflecting different degrees of magmatic evolution. There is also variability in terms of trace elements, particularly Rare Earth Elements (REEs), evidenced by decreasing (La/Sm)N, (Gd/Lu)N, (Ce/Yb)N and (Eu/Eu*)N towards the more evolved samples, which can be partly attributed to fractional crystallization processes. Most of the granitoids are characterized by (La/Yb)N = 3 - 10.8, showing negative Ba, Nb, Sr, Zr, P, Ti and Eu anomalies. On the other hand, microgranite dykes, along with a few more evolved granites, show an opposite tendency, being usually enriched in HREEs relatively to LREEs with (La/Yb)N = 0.4 - 1.1. Our data suggests intermediate genetic affinities between I-type and A-type granites. Although these granitoids are mostly metaluminous (characteristic of I-types), Ga/Al ratios, usually used to identify A-types, are close to the accepted boundary between A-type and other granite types. The affinities with A-type granites are more marked for the more evolved facies, which depict higher values of FeOt/MgO (14 - 60) and K2O/MgO (60 - 250). Their trace element characteristics are also transitional between WPG (Within-plate granites) and Syn-COLG (Collision Granites). We interpret those transitional characteristics (A/I and WPG/Syn-COLG) of Macao granitoids as reflecting an origin by melting of infracrustal sources over a period of high heat transfer from mantle to crust during an extensional tectonic setting probably contemporaneous with the subduction of the paleo-Pacific plate beneath the Eurasia, whose paleo-suture is thought to be located in the east flank of the Central Range, Taiwan. DA - 2016/12/01/ PY - 2016 VL - 11 J2 - AGU Fall Meeting Abstracts UR - http://adsabs.harvard.edu/abs/2016AGUFM.V11B2775Q Y2 - 2021/03/30/08:21:09 KW - 8499 General or miscellaneous KW - VOLCANOLOGY ER - TY - JOUR TI - Plasticity in reproductive behaviour in two populations of the peacock blenny AU - Oliveira, Rui F. AU - Simões, Mariana G. AU - Gonçalves, David AU - Saraiva, João L. T2 - Behaviour DA - 2011/// PY - 2011 DO - 10.1163/156853911X617089 DP - DOI.org (Crossref) VL - 148 IS - 14 SP - 1457 EP - 1472 J2 - Behav SN - 0005-7959, 1568-539X UR - https://brill.com/view/journals/beh/148/14/article-p1457_3.xml Y2 - 2021/02/19/09:02:52 ER - TY - JOUR TI - Macao air quality forecast using statistical methods AU - Lei, Man Tat AU - Monjardino, Joana AU - Mendes, Luisa AU - Gonçalves, David AU - Ferreira, Francisco T2 - Air Quality, Atmosphere & Health AB - The levels of air pollution in Macao often exceeded the levels recommended by WHO. In order for the population to take precautionary measures and avoid further health risks under high pollutant exposure, it is important to develop a reliable air quality forecast. Statistical models based on linear multiple regression (MR) and classification and regression trees (CART) analysis were developed successfully, for Macao, to predict the next day concentrations of NO2, PM10, PM2.5, and O3. All the developed models were statistically significantly valid with a 95% confidence level with high coefficients of determination (from 0.78 to 0.93) for all pollutants. The models utilized meteorological and air quality variables based on 5 years of historical data, from 2013 to 2017. Data from 2013 to 2016 were used to develop the statistical models and data from 2017 was used for validation purposes. A wide range of meteorological and air quality variables was identified, and only some were selected as significant independent variables. Meteorological variables were selected from an extensive list of variables, including geopotential height, relative humidity, atmospheric stability, and air temperature at different vertical levels. Air quality variables translate the resilience of the recent past concentrations of each pollutant and usually are maximum and/or the average of latest 24-h levels. The models were applied in forecasting the next day average daily concentrations for NO2 and PM and maximum hourly O3 levels for five air quality monitoring stations. The results are expected to be an operational air quality forecast for Macao. DA - 2019/09/01/ PY - 2019 DO - 10.1007/s11869-019-00721-9 DP - Springer Link VL - 12 IS - 9 SP - 1049 EP - 1057 J2 - Air Qual Atmos Health LA - en SN - 1873-9326 UR - https://doi.org/10.1007/s11869-019-00721-9 Y2 - 2022/09/21/08:23:38 KW - Macao KW - NO2 KW - O3 KW - PM10 KW - PM2.5 KW - Particulate matter ER - TY - JOUR TI - Macao air quality forecast using statistical methods AU - Lei, Man Tat AU - Monjardino, Joana AU - Mendes, Luisa AU - Ferreira, Francisco T2 - International Journal of Environmental Impacts: Management, Mitigation and Recovery DA - 2019/07/19/ PY - 2019 DO - 10.2495/EI-V2-N3-249-258 DP - DOI.org (Crossref) VL - 2 IS - 3 SP - 249 EP - 258 J2 - Int. J. EI LA - en SN - 2398-2640, 2398-2659 UR - http://www.witpress.com/doi/journals/EI-V2-N3-249-258 Y2 - 2022/09/21/08:23:59 ER - TY - JOUR TI - Characterization of the Natural Soundscape of Zebrafish and Comparison with the Captive Noise Conditions AU - Lara, Rafael A. AU - Vasconcelos, Raquel O. T2 - Zebrafish AB - Zebrafish is a well-established model organism in hearing research. Although the acoustic environment is known to shape the structure and sensitivity of auditory systems, there is no information on the natural soundscape of this species. Moreover, zebrafish are typically reared in large-scale housing systems (HS), although their acoustic properties and potential effects on hearing remain unknown. We characterized the soundscape of both zebrafish natural habitats and laboratory captive conditions, and discussed possible impact on auditory sensitivity. Sound recordings were conducted in five distinct zebrafish habitats (Southwest India), from quieter stagnant environments with diverse biological/abiotic sounds to louder watercourses characterized by current and moving substrate sounds. Sound pressure level (SPL) varied between 98 and 126 dB re 1 μPa. Sound spectra presented most energy below 3000 Hz and quieter noise windows were found in the noisiest habitats matching the species best hearing range. Contrastingly, recordings from three zebrafish HS revealed higher SPL (122-143 dB) and most energy below 1000 Hz with more spectral peaks, which might cause significant auditory masking. This study establishes an important ground for future research on the adaptation of zebrafish auditory system to the natural soundscapes, and highlights the importance of controlling noise conditions in captivity. DA - 2019/04// PY - 2019 DO - 10.1089/zeb.2018.1654 DP - PubMed VL - 16 IS - 2 SP - 152 EP - 164 J2 - Zebrafish LA - eng SN - 1557-8542 KW - Animals KW - Animals, Laboratory KW - Auditory Threshold KW - Batrachoidiformes KW - Ecosystem KW - Environment KW - Female KW - Hair Cells, Auditory KW - Hearing KW - Housing, Animal KW - India KW - Male KW - Neuronal Plasticity KW - Noise KW - Saccule and Utricle KW - Seasons KW - Sexual Behavior, Animal KW - Sound KW - Species Specificity KW - Vocalization, Animal KW - Zebrafish KW - ambient noise KW - hearing sensitivity KW - natural habitat KW - sound pressure level KW - soundscape ER - TY - JOUR TI - Uptake and depuration kinetics of dicofol metabolite 4,4′-dichlorobenzophenone, in the edible Asiatic clam Meretrix meretrix AU - Ivorra, Lucia AU - Cruzeiro, Catarina AU - Chan, Shek Kiu AU - Tagulao, Karen Araño AU - Cardoso, Patricia G. T2 - Chemosphere AB - Uptake and depuration kinetics of 4,4′-dichlorobenzophenone (main metabolite of dicofol) in the edible clam Meretrix meretrix were evaluated through a mesocosm experiment. M. meretrix was exposed to different dicofol concentrations (environmental concentration, D1 = 50 ng/L; supra-environmental concentration, D2 = 500 ng/L) for 15 days, followed by the same depuration period. To accomplish this goal, an analytical method was successfully optimized for 4,4′-DCBP using QuEChERS as extraction method with a range of concentrations 0.3–76.8 ng/g ww quantified by gas chromatography coupled to tandem mass spectrometry. Our results demonstrated different kinetics of accumulation depending on the two dicofol treatments. For D1, the uptake kinetic was best fitted using a plateau followed by one phase association kinetic model, while for D2 a one phase association kinetic model suited better. Similar bioconcentration factors were obtained for both concentrations but only animals exposed to D2, showed 4,4′-DCBP levels above the limits of quantification after 24 h exposure. These animals also showed lower uptake rate (ku) than organisms exposed to D1. During the depuration period, only organisms exposed to D1 successfully depurated after 24 h. On the other hand, although animals exposed to D2 presented higher elimination factor, they did not reach the original levels after depuration. Moreover, values detected in these clams were higher than the Maximum Residue Level (10 ng/g) established by the European legislation. This indicates that longer periods of depuration time than the ones used in this study, may be needed in order to reach safe levels for human consumption. This work also demonstrated that studies on metabolite kinetics during uptake/depuration experiments, could be a new alternative to understand the impact and metabolism of pesticides in the marine environment. DA - 2019/11/01/ PY - 2019 DO - 10.1016/j.chemosphere.2019.06.155 DP - ScienceDirect VL - 235 SP - 662 EP - 669 J2 - Chemosphere LA - en SN - 0045-6535 UR - https://www.sciencedirect.com/science/article/pii/S0045653519313967 Y2 - 2022/11/09/10:10:30 KW - Bivalves KW - GC-MS/MS KW - Organochlorine pesticides KW - QuEChERS bioconcentration KW - Seafood ER - TY - JOUR TI - Environmental characterization of 4,4′-dichlorobenzophenone in surface waters from Macao and Hong Kong coastal areas (Pearl River Delta) and its toxicity on two biological models: Artemia salina and Daphnia magna AU - Ivorra, Lucia AU - Cardoso, Patricia G. AU - Chan, Shek Kiu AU - Tagulao, Karen AU - Cruzeiro, Catarina T2 - Ecotoxicology and Environmental Safety DA - 2019/04// PY - 2019 DO - 10.1016/j.ecoenv.2018.12.054 DP - DOI.org (Crossref) VL - 171 SP - 1 EP - 11 J2 - Ecotoxicology and Environmental Safety LA - en SN - 01476513 ST - Environmental characterization of 4,4′-dichlorobenzophenone in surface waters from Macao and Hong Kong coastal areas (Pearl River Delta) and its toxicity on two biological models UR - https://linkinghub.elsevier.com/retrieve/pii/S0147651318313472 Y2 - 2022/11/09/10:05:07 ER - TY - CHAP TI - Hormones and Sexual Behavior of Teleost Fishes AU - Gonçalves, David AU - Oliveira, Rui F. T2 - Hormones and Reproduction of Vertebrates DA - 2011/// PY - 2011 DP - DOI.org (Crossref) SP - 119 EP - 147 LA - en PB - Elsevier SN - 978-0-12-375009-9 UR - https://linkinghub.elsevier.com/retrieve/pii/B9780123750099100074 Y2 - 2021/02/19/09:02:07 ER - TY - CHAP TI - Neuroendocrinology of Social Behavior in Teleost Fish AU - Gonçalves, David AU - Félix, Ana S. AU - Oliveira, Rui F. T2 - Hormones, Brain and Behavior DA - 2017/// PY - 2017 SP - 3 EP - 18 LA - en PB - Elsevier SN - 978-0-12-803608-2 UR - https://linkinghub.elsevier.com/retrieve/pii/B9780128035924000201 Y2 - 2021/02/19/08:50:32 ER - TY - RPRT TI - Behavioral plasticity meets neuroplasticity: brain neurogenesis in the polymorphic fish Salaria pavo AU - Gonçalves, David Manuel Flores CY - Macao DA - 2016/// PY - 2016 M3 - Research Report PB - University of Saint Joseph ER - TY - JOUR TI - Alternative pathway history in a fish with plastic reproductive tactic AU - Gonçalves, David T2 - Functional Ecology DA - 2014/// PY - 2014 ER - TY - RPRT TI - DISRUPT - Environmental Endocrine disruptors: Current situation in Macao, neurobehavioral effects and bioremediation strategies AU - Gonçalves, David CY - Macao DA - 2018/// PY - 2018 M3 - Research Report PB - University of Saint Joseph ER - TY - RPRT TI - FIGHT – Hormonal, pheromonal and genomic regulation of aggression in a fish model AU - Gonçalves, David CY - Macao DA - 2018/// PY - 2018 M3 - Research Report PB - University of Saint Joseph ER - TY - JOUR TI - Oestradiol and prostaglandin F2α regulate sexual display in female of a sex-role reversed fish AU - Gonçalves, David DA - 2014/// PY - 2014 VL - 281 IS - 1778 ER - TY - JOUR TI - Chromosome-level reference genome of the Siamese fighting fish Betta splendens, a model species for the study of aggression AU - Fan, Guangyi AU - Chan, Judy AU - Ma, Kailong AU - Yang, Binrui AU - Zhang, He AU - Yang, Xianwei AU - Shi, Chengcheng AU - Law, Henry AU - Ren, Zhitao AU - Xu, Qiwu AU - Liu, Qun AU - Wang, Jiahao AU - Chen, Wenbin AU - Shao, Libin AU - Gonçalves, David AU - Ramos, Andreia AU - Cardoso, Sara D AU - Guo, Min AU - Cai, Jing AU - Xu, Xun AU - Wang, Jian AU - Yang, Huanming AU - Liu, Xin AU - Wang, Yitao T2 - GigaScience DA - 2018/07/11/ PY - 2018 DO - 10.1093/gigascience/giy087 LA - en SN - 2047-217X UR - https://academic.oup.com/gigascience/advance-article/doi/10.1093/gigascience/giy087/5054042 Y2 - 2021/02/19/08:48:24 ER - TY - JOUR TI - Birth date predicts alternative life‐history pathways in a fish with sequential reproductive tactics AU - Fagundes, Teresa AU - Simões, Mariana G. AU - Saraiva, João L. AU - Ros, Albert F. H. AU - Gonçalves, David AU - Oliveira, Rui F. T2 - Functional Ecology A2 - Grindstaff, Jennifer DA - 2015/12// PY - 2015 DO - 10.1111/1365-2435.12465 VL - 29 IS - 12 SP - 1533 EP - 1542 J2 - Funct Ecol LA - en SN - 0269-8463, 1365-2435 UR - https://onlinelibrary.wiley.com/doi/abs/10.1111/1365-2435.12465 Y2 - 2021/02/19/08:51:47 ER - TY - JOUR TI - Social cues in the expression of sequential alternative reproductive tactics in young males of the peacock blenny, Salaria pavo AU - Fagundes, Teresa AU - Simões, Mariana G. AU - Gonçalves, David AU - Oliveira, Rui F. T2 - Physiology & Behavior DA - 2012/10// PY - 2012 DO - 10.1016/j.physbeh.2012.07.011 VL - 107 IS - 3 SP - 283 EP - 291 J2 - Physiology & Behavior LA - en SN - 00319384 UR - https://linkinghub.elsevier.com/retrieve/pii/S0031938412002740 Y2 - 2021/02/19/09:01:16 ER - TY - CONF TI - Achieving acceptable air quality in Macao through sustainable transportation AU - Ezeka, Franklin A.N AU - Chan, Sek Kiu T2 - International symposium on the progrees of technology in environmental impact assessment, 2010 Macao international environmental co-operation forum & exhibition C1 - Macao SAR, China DA - 2010/04/10/ PY - 2010 ER - TY - CONF TI - Geochronological, geochemical and petrographic constraints on incremental pluton growth: the case of Macao granitic suite AU - Dias, Ágata AU - Quelhas, P AU - Mata, J AU - Wayne, Davis T2 - Goldschmidt C1 - Barcelona DA - 2019/// PY - 2019 UR - https://goldschmidt.info/2019/abstracts/abstractView?id=2019004459 ER - TY - CONF TI - A origem e evolução dos magmas graníticos de Macao à luz de dados de geoquímica elementar e isotópica / Source and evolution of Macao granitic magmas: insights from wholerock geochemistry and isotopic signatures. AU - Dias, Ágata AU - Quelhas, P AU - Mata, J AU - Lou, U T AU - Ribeiro, M L AU - Borges, R T2 - XIV Congresso de Geoquímica dos Países de Língua Portuguesa e XIX Semana de Geoquímica (XIV CGPLP/XIX SG, international conference C1 - Trás-os-Montes e Alto Douro, Portugal DA - 2018/// PY - 2018 ER - TY - CONF TI - New geochemical constraints on I-type granites of Macao: evidences for fractional crystallization and petrogenetic affinities with Jurassic-Cretaceous granites in SE China AU - Dias, Ágata AU - Quelhas, P AU - Mata, J AU - Lou, U T AU - Borges, R AU - Ribeiro, M. L T2 - Goldschmidt DA - 2017/// PY - 2017 UR - https://goldschmidtabstracts.info/2017/3248.pdf ER - TY - CONF TI - Petrology and Geochemistry of Granitic Rocks from Macao AU - Dias, Ágata AU - Quelhas, P AU - Lou, U AU - Mata, J AU - Ribeiro, M L T2 - Goldschmidt C1 - Yokohama, Japan DA - 2016/// PY - 2016 UR - https://goldschmidtabstracts.info/2016/665.pdf ER - TY - CONF TI - Sulfide mineralization of the Saldanha hydrothermal field (MAR): constraints from sulfur isotope in-situ microanalysis AU - Dias, Ágata AU - Qiu, W AU - Barriga, F AU - Tao, C T2 - InterRidge Workshop on Hydrothermal Ore-forming Processes C1 - Hangzhou DA - 2019/// PY - 2019 ER - TY - JOUR TI - Surface sediment composition and distribution of hydrothermal derived elements at the Duanqiao-1 hydrothermal field, Southwest Indian Ridge AU - Dias, Ágata AU - Liao, Shili AU - Tao, Chunhui AU - Su, Xin AU - Yang, Zhen AU - Ni, Jianyu AU - Liang, Jin AU - Yang, Weifang AU - Liu, Jia AU - Li, Wei AU - Dong, Chuanwan T2 - Marine Geology DA - 2019/10// PY - 2019 DO - 10.1016/j.margeo.2019.105975 VL - 416 SP - 105975 J2 - Marine Geology LA - en SN - 00253227 UR - https://linkinghub.elsevier.com/retrieve/pii/S0025322719300969 Y2 - 2021/03/30/07:30:43 ER - TY - JOUR TI - Geochemistry and stable isotope constraints on high-temperature activity from sediment cores of the Saldanha hydrothermal field AU - Dias, Ágata AU - Früh-Green, G.L. AU - Bernasconi, S.M. AU - Barriga, F.J.A.S. T2 - Marine Geology DA - 2011/01// PY - 2011 DO - 10.1016/j.margeo.2010.10.017 VL - 279 IS - 1-4 SP - 128 EP - 140 J2 - Marine Geology LA - en SN - 00253227 UR - https://linkinghub.elsevier.com/retrieve/pii/S0025322710002884 Y2 - 2021/03/30/07:34:19 ER - TY - CONF TI - Geochemistry of Fe-Si-(Mn) Chimneys from Luso Vent Field, MAR AU - Dias, Ágata AU - Costa, P AU - Marques, A F AU - Ribeiro, L AU - Madureira, P AU - Calado, A AU - Gonçalves, E AU - Morato, T T2 - Goldschmidt C1 - Barcelona DA - 2019/// PY - 2019 UR - https://goldschmidt.info/2019/abstracts/abstractView?id=2019003072 ER - TY - CONF TI - Saldanha hydrothermal field: the role of the sediment-cover in the preservation of ore-forming minerals AU - Dias, Ágata AU - Barriga, F T2 - Third InterRidge Theoretical Institute: Magmatic and Tectonic Processes and Seabed Resources at Mid-Ocean Ridges. T2-09 C1 - Hangzhou, China DA - 2015/// PY - 2015 ER - TY - CONF TI - Diving into the unknown: robotics as tools to study the deep-sea. Informed Scientific Sampling in Large-scale Outdoor Environments AU - Dias, Ágata T2 - International Conference on Intelligent Robots and Systems IROS C1 - Macau DA - 2019/// PY - 2019 ER - TY - RPRT TI - MaGIC – Petrology and Geochemistry of Igneous Rocks from Macao: Implications for the Crustal Evolution of Southern China. AU - Dias, Ágata CY - Macao DA - 2017/// PY - 2017 M3 - Research Report PB - University of Saint Joseph ER - TY - RPRT TI - Petrology and Geochemistry of Igneous Rocks from Macao: Implications for the Crustal Evolution of Southern China AU - Dias, Ágata CY - Macao DA - 2016/// PY - 2016 M3 - Research Report PB - University of Saint Joseph ER - TY - RPRT TI - Petrology and Geochemistry of Igneous Rocks from Macao: Implications for the Crustal Evolution of Southern China AU - Dias, Ágata CY - Macao DA - 2018/// PY - 2018 M3 - Research Report PB - University of Saint Joseph ER - TY - RPRT TI - Research Projects: Petrology and Geochemistry of Igneous Rocks from Macao: Implications for the Crustal Evolution of Southern China AU - Dias, Ágata CY - Macao DA - 2018/// PY - 2018 M3 - Research Report PB - University of Saint Joseph ER - TY - CONF TI - Sr and Nd isotopic composition of Jan Mayen and Loki’s Castle: a progress report AU - Cruz, M I AU - Marques, F A M AU - Dias, Ágata AU - Pedersen, R AU - Barriga, F T2 - 43rd Underwater Mining Institute DA - 2014/// PY - 2014 ER - TY - CONF TI - Sulfide Sites in the Arctic Ocean: Jan Mayen and Loki’s Castle, Mineralogical Magazine AU - Cruz, M I AU - Marques, A F AU - Dias, Ágata AU - Pedersen, R AU - Relvas, J M R S AU - Barriga, F J A S T2 - Goldschmidt DA - 2013/// PY - 2013 UR - https://goldschmidtabstracts.info/abstracts/abstractView?id=2013003606 ER - TY - CONF TI - Sulphide mineralization in Arctic seafloor hydrothermal fields: extending the European exploration area to the Far North AU - Cruz, M I AU - Dias, Ágata AU - Pedersen, R B AU - Relvas, J M R S AU - Fonseca, R AU - Barriga, F J A S T2 - ERA-MIN. DA - 2013/// PY - 2013 ER - TY - CONF TI - Hydrothermal Fe-Mn deposits from low-temperature systems of the Mid-Atlantic Ridge AU - Costa, P AU - Dias, Ágata T2 - InterRidge Workshop on Hydrothermal Ore-forming Processes C1 - Hangzhou DA - 2019/// PY - 2019 ER - TY - JOUR TI - Use of stable isotopes to understand food webs in Macao wetlands AU - Chen, Qian AU - Liu, Yang AU - Ho, Wei-Tim AU - Chan, Shek Kiu AU - Li, Qiu-hua AU - Huang, Jian-Rong T2 - Wetlands Ecology and Management AB - In this study, components of the food-web in Macao wetlands were quantified using stable isotope ratio techniques based on carbon and nitrogen values. The δ13C and δ15N values of particulate organic matter (δ13CPOM and δ15NPOM, respectively) ranged from −30.64 ± 1.0 to −28.1 ± 0.7 ‰, and from −1.11 ± 0.8 to 3.98 ± 0.7 ‰, respectively. The δ13C values of consumer species ranged from −33.94 to −16.92 ‰, showing a wide range from lower values in a freshwater lake and inner bay to higher values in a mangrove forest. The distinct dietary habits of consumer species and the location-specific food source composition were the main factors affecting the δ13C values. The consumer 15N-isotope enrichment values suggested that there were three trophic levels; primary, secondary, and tertiary. The primary consumer trophic level was represented by freshwater herbivorous gastropods, filter-feeding bivalves, and plankton-feeding fish, with a mean δ15N value of 5.052 ‰. The secondary consumer level included four deposit-feeding fish species distributed in Fai Chi Kei Bay and deposit-feeding gastropods in the Lotus Flower Bridge flat, with a mean δ15N value of 6.794 ‰. The tertiary consumers group consisted of four crab species, one shrimp species, and four fish species in the Lotus Flower Bridge Flat, with a mean δ15N value of 13.473 ‰. Their diet mainly comprised organic debris, bottom fauna, and rotten animal tissues. This study confirms the applicability of the isotopic approach in food web studies. DA - 2017/02/01/ PY - 2017 DO - 10.1007/s11273-016-9502-2 DP - Springer Link VL - 25 IS - 1 SP - 59 EP - 66 J2 - Wetlands Ecol Manage LA - en SN - 1572-9834 UR - https://doi.org/10.1007/s11273-016-9502-2 Y2 - 2021/02/10/09:01:46 ER - TY - JOUR TI - Ontogenetic development of the inner ear saccule and utricle in the Lusitanian toadfish: Potential implications for auditory sensitivity AU - Chaves, Patrícia P. AU - Valdoria, Ciara M. C. AU - Amorim, M. Clara P. AU - Vasconcelos, Raquel O. T2 - Hearing Research AB - Studies addressing structure-function relationships of the fish auditory system during development are sparse compared to other taxa. The Batrachoididae has become an important group to investigate mechanisms of auditory plasticity and evolution of auditory-vocal systems. A recent study reported ontogenetic improvements in the inner ear saccule sensitivity of the Lusitanian toadfish, Halobatrachus didactylus, but whether this results from changes in the sensory morphology remains unknown. We investigated how the macula and organization of auditory receptors in the saccule and utricle change during growth in this species. Inner ear sensory epithelia were removed from the end organs of previously PFA-fixed specimens, from non-vocal posthatch fry (<1.4 cm, standard length) to adults (>23 cm). Epithelia were phalloidin-stained and analysed for area, shape, number and orientation patterns of hair cells (HC), and number and size of saccular supporting cells (SC). Saccular macula area expanded 41x in total, and significantly more (relative to body length) among vocal juveniles (2.3–2.9 cm). Saccular HC number increased 25x but HC density decreased, suggesting that HC addition is slower relative to epithelial growth. While SC density decreased, SC apical area increased, contributing to the epithelial expansion. The utricule revealed increased HC density (striolar region) and less epithelial expansion (5x) with growth, contrasting with the saccule that may have a different developmental pattern due to its larger size and main auditory functions. Both macula shape and HC orientation patterns were already established in the posthatch fry and retained throughout growth in both end organs. We suggest that previously reported ontogenetic improvements in saccular sensitivity might be associated with changes in HC number (not density), size and/or molecular mechanisms controlling HC sensitivity. This is one of the first studies investigating the ontogenetic development of the saccule and utricle in a vocal fish and how it potentially relates to auditory enhancement for acoustic communication. DA - 2017/09/01/ PY - 2017 DO - 10.1016/j.heares.2017.06.008 DP - ScienceDirect VL - 353 SP - 112 EP - 121 J2 - Hearing Research LA - en SN - 0378-5955 ST - Ontogenetic development of the inner ear saccule and utricle in the Lusitanian toadfish UR - https://www.sciencedirect.com/science/article/pii/S0378595517300977 Y2 - 2021/02/10/10:30:58 KW - Auditory sensitivity KW - Batrachoididae KW - Hair cell KW - Ontogeny KW - Saccule KW - Utricle ER - TY - RPRT TI - The role of mangroves on the bioaccumulation and citogenotoxic effects of metals and pesticides on the food web of a sub-tropical coastal system AU - Chan, Shek Kiu AU - Tagulao, Karen CY - Macao DA - 2018/// PY - 2018 M3 - Research Report PB - University of Saint Joseph ER - TY - RPRT TI - The role of mangroves on the bioaccumulation and citogenotoxic effects of metals and pesticides on the food web of a tropical coastal system AU - Chan, Shek Kiu AU - Tagulao, Karen CY - Macao DA - 2016/// PY - 2016 M3 - Research Report PB - University of Saint Joseph ER - TY - RPRT TI - The Role of Mangroves on the Bioaccumulation and Citogenotoxic Effects of Metals and Pesticides on the Food Web of a Coastal System AU - Chan, S. K AU - Tagulao, Karen CY - Macao DA - 2016/// PY - 2016 M3 - Research Report PB - University of Saint Joseph ER - TY - JOUR TI - Neurogenomic mechanisms of social plasticity AU - Cardoso, Sara D. AU - Teles, Magda C. AU - Oliveira, Rui F. T2 - Journal of Experimental Biology AB - Skip to Next Section Group-living animals must adjust the expression of their social behaviour to changes in their social environment and to transitions between life-history stages, and this social plasticity can be seen as an adaptive trait that can be under positive selection when changes in the environment outpace the rate of genetic evolutionary change. Here, we propose a conceptual framework for understanding the neuromolecular mechanisms of social plasticity. According to this framework, social plasticity is achieved by rewiring or by biochemically switching nodes of a neural network underlying social behaviour in response to perceived social information. Therefore, at the molecular level, it depends on the social regulation of gene expression, so that different genomic and epigenetic states of this brain network correspond to different behavioural states, and the switches between states are orchestrated by signalling pathways that interface the social environment and the genotype. Different types of social plasticity can be recognized based on the observed patterns of inter- versus intra-individual occurrence, time scale and reversibility. It is proposed that these different types of social plasticity rely on different proximate mechanisms at the physiological, neural and genomic level. DA - 2015/01/01/ PY - 2015 DO - 10.1242/jeb.106997 DP - jeb.biologists.org VL - 218 IS - 1 SP - 140 EP - 149 LA - en SN - 0022-0949, 1477-9145 UR - https://jeb.biologists.org/content/218/1/140 Y2 - 2021/02/10/10:45:13 ER - TY - JOUR TI - Efficient isolation of polymorphic microsatellites from high-throughput sequence data based on number of repeats AU - Cardoso, Sara D. AU - Gonçalves, David AU - Robalo, Joana I. AU - Almada, Vitor C. AU - Canário, Adelino V.M. AU - Oliveira, Rui F. T2 - Marine Genomics DA - 2013/09// PY - 2013 DO - 10.1016/j.margen.2013.04.002 VL - 11 SP - 11 EP - 16 J2 - Marine Genomics LA - en SN - 18747787 UR - https://linkinghub.elsevier.com/retrieve/pii/S1874778713000226 Y2 - 2021/02/19/08:56:47 ER - TY - JOUR TI - Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish AU - Cardoso, Sara D. AU - Gonçalves, David AU - Goesmann, Alexander AU - Canário, Adelino V. M. AU - Oliveira, Rui F. T2 - Molecular Ecology AB - Distinct patterns of gene expression often underlie intra- and intersexual differences, and the study of this set of coregulated genes is essential to understand the emergence of complex behavioural phenotypes. Here, we describe the development of a de novo transcriptome and brain gene expression profiles of wild-caught peacock blenny, Salaria pavo, an intertidal fish with sex-role reversal in courtship behaviour (i.e., females are the courting sex) and sequential alternative reproductive tactics in males (i.e., larger and older nest-holder males and smaller and younger sneaker males occur). Sneakers mimic both female's courtship behaviour and nuptial coloration to get access to nests and sneak fertilizations, and later in life transition into nest-holder males. Thus, this species offers the unique opportunity to study how the regulation of gene expression can contribute to intersex phenotypes and to the sequential expression of male and female behavioural phenotypes by the same individual. We found that at the whole brain level, expression of the sneaker tactic was paralleled by broader and divergent gene expression when compared to either females or nest-holder males, which were more similar between themselves. When looking at sex-biased transcripts, sneaker males are intersex rather than being either nest-holder or female-like, and their transcriptome is simultaneously demasculinized for nest-holder-biased transcripts and feminized for female-biased transcripts. These results indicate that evolutionary changes in reproductive plasticity can be achieved through regulation of gene expression, and in particular by varying the magnitude of expression of sex-biased genes, throughout the lifetime of the same individual. DA - 2018/// PY - 2018 DO - https://doi.org/10.1111/mec.14408 VL - 27 IS - 3 SP - 789 EP - 803 LA - en SN - 1365-294X UR - https://onlinelibrary.wiley.com/doi/abs/10.1111/mec.14408 Y2 - 2021/02/10/10:37:37 KW - RNA-seq KW - Salaria pavo KW - alternative reproductive tactics KW - behavioural plasticity KW - neurogenomic state KW - sex-biased gene expression ER - TY - JOUR TI - Social network predicts loss of fertilizations in nesting males of a fish with alternative reproductive tactics AU - Cardoso, Sara D. AU - Faustino, Ana I. AU - Costa, Silvia S. AU - Valério, Fábio AU - Gonçalves, David AU - Oliveira, Rui F. T2 - acta ethologica AB - Alternative reproductive tactics (ARTs) evolve when there is strong intra-sexual competition between conspecifics for access to mates. Typically, larger “bourgeois” males reproduce by securing the access to reproductive resources while smaller “parasitic” males reproduce by stealing fertilizations from larger males. A number of factors can influence the reproductive success of each tactic, including intrinsic (e.g. size) and extrinsic (e.g. tactic relative frequency) variables. An example where plastic ARTs occur is the peacock blenny Salaria pavo, with large males reproducing by defending nests and attracting females (bourgeois tactic) and small males reproducing by achieving sneaked fertilizations (parasitic tactic). In this study, we conducted field observations on individually tagged animals to determine their social network and collected eggs from 11 nests to determine the fertilization success of each male tactic. Paternity estimates for 550 offspring indicated an average fertilization success for nest-holder males of 95%. Nest-holder male morphological traits and social network parameters were tested as predictors of fertilization success, but only the number of sneakers present in the nest-holder’s social networks was found to be a predictor of paternity loss. Although male morphological traits had been previously found to be strongly correlated with reproductive success of nest-holder males, as measured by the number of eggs collected in the male’s nest, no correlation was found between any of the measured morphological traits and fertilization success for these males. The results suggest a stronger influence of the social environment than of morphological variables in the proportion of lost fertilizations by nest-holder males of this species. DA - 2017/02/01/ PY - 2017 DO - 10.1007/s10211-016-0249-9 VL - 20 IS - 1 SP - 59 EP - 68 J2 - acta ethol LA - en SN - 1437-9546 UR - https://doi.org/10.1007/s10211-016-0249-9 Y2 - 2021/02/01/05:08:39 ER - TY - JOUR TI - Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequen-tial male alternative reproductive tactic in fish. AU - Cardoso, S.D., Gonçalves, D., Goesmann, A., Canário, A.V.M., & Oliveira, R.F. T2 - Molecular Ecology DA - 2017/// PY - 2017 DO - : 10.1111/mec.14408 ER - TY - JOUR TI - Potential Ecological Risk of Heavy Metal Distribution in Cemetery Soils AU - Amuno, S. A. T2 - Water, Air, & Soil Pollution AB - In this paper, preliminary investigation was conducted to evaluate the potential ecological risk of heavy metals contamination in cemetery soils. Necrosol samples were collected from within and around the vicinity of the largest mass grave in Rwanda and analyzed for heavy metal concentrations using total digestion–inductively coupled plasma mass spectrometry and instrumental neutron activation analysis. Based on the concentrations of As, Cu, Cr, Pb, and Zn, the overall contamination degree (Cdeg) and potential ecological risks status (RI) of the necrosols were determined. The preliminary results revealed that the associated cemetery soils are only contaminated to a low degree. On the other hand, assessment of the potential ecological risk index (RI) revealed that cumulative heavy metal content of the soil do not pose any significant ecological risks. These findings, therefore, suggest that, while cemetery soils may be toxic due to the accumulation of certain heavy metals, their overall ecological risks may be minimal and insignificant. DA - 2013/01/23/ PY - 2013 DO - 10.1007/s11270-013-1435-2 DP - Springer Link VL - 224 IS - 2 SP - 1435 J2 - Water Air Soil Pollut LA - en SN - 1573-2932 UR - https://doi.org/10.1007/s11270-013-1435-2 Y2 - 2023/04/11/12:11:27 KW - Cemetery soils KW - Environmental management KW - Heavy metals KW - Mass graves ER - TY - JOUR TI - Acoustic communication in marine shallow waters: testing the acoustic adaptive hypothesis in sand gobies AU - Amorim, Maria Clara P. AU - Vasconcelos, Raquel O. AU - Bolgan, Marta AU - Pedroso, Silvia S. AU - Fonseca, Paulo J. T2 - Journal of Experimental Biology AB - Skip to Next Section Acoustic communication is an important part of social behaviour of fish species that live or breed in shallow noisy waters. Previous studies have shown that some fish species exploit a quiet window in the background noise for communication. However, it remains to be examined whether hearing abilities and sound production of fish are adapted to marine habitats presenting high hydrodynamism. Here, we investigated whether the communication system of the painted (Pomatoschistus pictus) and the marbled (Pomatoschistus marmoratus) gobies is adapted to enhance sound transmission and reception in Atlantic shallow water environments. We recorded and measured the sound pressure levels of social vocalisations of both species, as well as snapshots of ambient noise of habitats characterised by different hydrodynamics. Hearing thresholds (in terms of both sound pressure and particle acceleration) and responses to conspecific signals were determined using the auditory evoked potential recording technique. We found that the peak frequency range (100–300 Hz) of acoustic signals matched the best hearing sensitivity in both species and appeared well adapted for short-range communication in Atlantic habitats. Sandy/rocky exposed beaches presented a quiet window, observable even during the breaking of moderate waves, coincident with the main sound frequencies and best hearing sensitivities of both species. Our data demonstrate that the hearing abilities of these gobies are well suited to detect conspecific sounds within typical interacting distances (a few body lengths) in Atlantic shallow waters. These findings lend support to the acoustic adaptive hypothesis, under the sensory drive framework, proposing that signals and perception systems coevolve to be effective within local environment constraints. DA - 2018/11/15/ PY - 2018 DO - 10.1242/jeb.183681 DP - jeb.biologists.org VL - 221 IS - 22 LA - en SN - 0022-0949, 1477-9145 ST - Acoustic communication in marine shallow waters UR - https://jeb.biologists.org/content/221/22/jeb183681 Y2 - 2021/02/10/10:49:30 ER - TY - CHAP TI - Fish Sounds and Mate Choice AU - Amorim, M. Clara P. AU - Vasconcelos, Raquel O. AU - Fonseca, Paulo J. T2 - Sound Communication in Fishes A2 - Ladich, Friedrich T3 - Animal Signals and Communication AB - Fish acoustic signals associated with mating behaviour are typically low-frequency sounds produced by males when in close proximity to females. However, some species make sounds that serve the function and follow the design of advertisement calls, well known in insects, anurans, and birds. Close-range courtship acoustic signals may be used by females in mate assessment as they contain information of male quality such as size and condition. For example, sound-dominant frequency, amplitude, and fatigue resistance may signal body size whereas pulse period (i.e. muscle contraction rate) and calling activity are related with body condition in some species. Some signal features, such as sound pulse number, may carry multiple messages including size and condition. Playback experiments on mate choice of a restricted number of species suggest that females prefer vocal to silent males and may use sound frequency, amplitude, and mainly calling rateCalling ratewhen assessing males. The assessment of males by females becomes more challenging when males engage in choruses or when sounds are otherwise masked by anthropogenic noise but almost nothing is known about how these aspects affect mating decisions and fish reproductive success. CY - Vienna DA - 2015/// PY - 2015 DP - Springer Link SP - 1 EP - 33 LA - en PB - Springer SN - 978-3-7091-1846-7 UR - https://doi.org/10.1007/978-3-7091-1846-7_1 Y2 - 2022/09/21/08:16:30 KW - Acoustic communication KW - Anthropogenic noise KW - Calling activity KW - Chorus KW - Fish KW - Mate choice KW - Reproductive success ER -