TY - THES TI - Impact of Vessel Traffic on Underwater Soundscape and Marine Mammals in the Pearl River Delta AU - Wong, Man Ieng A3 - Vasconcelos, Raquel A3 - Porter, Lindsay CN - M-ESM 2021 WON,MAN CY - Macau DA - 2021/// PY - 2021 DP - Library Catalog (Koha) SP - 64 LA - eng PB - University of Saint Joseph UR - https://library-opac.usj.edu.mo/cgi-bin/koha/opac-detail.pl?biblionumber=177012&query_desc=an%3A83478 KW - Master of Environmental Sciences and Management (MESM) KW - University of Saint Joseph ER - TY - JOUR TI - The effect of time regime in noise exposure on the auditory system and behavioural stress in the zebrafish AU - Wong, Man Ieng AU - Lau, Ieng Hou AU - Gordillo-Martinez, Flora AU - Vasconcelos, Raquel O. T2 - Scientific Reports AB - Anthropogenic noise of variable temporal patterns is increasing in aquatic environments, causing physiological stress and sensory impairment. However, scarce information exists on exposure effects to continuous versus intermittent disturbances, which is critical for noise sustainable management. We tested the effects of different noise regimes on the auditory system and behaviour in the zebrafish (Danio rerio). Adult zebrafish were exposed for 24 h to either white noise (150 ± 10 dB re 1 μPa) or silent control. Acoustic playbacks varied in temporal patterns—continuous, fast and slow regular intermittent, and irregular intermittent. Auditory sensitivity was assessed with Auditory Evoked Potential recordings, revealing hearing loss and increased response latency in all noise-treated groups. The highest mean threshold shifts (c. 13 dB) were registered in continuous and fast intermittent treatments, and no differences were found between regular and irregular regimes. Inner ear saccule did not reveal significant hair cell loss but showed a decrease in presynaptic Ribeye b protein especially after continuous exposure. Behavioural assessment using the standardized Novel Tank Diving assay showed that all noise-treated fish spent > 98% time in the bottom within the first minute compared to 82% in control, indicating noise-induced anxiety/stress. We provide first data on how different noise time regimes impact a reference fish model, suggesting that overall acoustic energy is more important than regularity when predicting noise effects. DA - 2022/09/12/ PY - 2022 DO - 10.1038/s41598-022-19573-y DP - www.nature.com VL - 12 IS - 1 SP - 15353 J2 - Sci Rep LA - en SN - 2045-2322 UR - https://www.nature.com/articles/s41598-022-19573-y Y2 - 2022/09/21/08:11:43 KW - Ecology KW - Neuroscience KW - Physiology KW - Zoology ER -