TY - CHAP TI - X-Ray Machine Learning Classification with VGG-16 for Feature Extraction AU - dos Santos Silva, Bruno Riccelli AU - Cortez, Paulo Cesar AU - da Silva Neto, Manuel Gonçalves AU - Lobo Marques, Joao Alexandre T2 - Computerized Systems for Diagnosis and Treatment of COVID-19 A2 - Lobo Marques, Joao Alexandre A2 - Fong, Simon James AB - The Covid-19 pandemic evidenced the need Computer Aided Diagnostic Systems to analyze medical images, such as CT and MRI scans and X-rays, to assist specialists in disease diagnosis. CAD systems have been shown to be effective at detecting COVID-19 in chest X-ray and CT images, with some studies reporting high levels of accuracy and sensitivity. Moreover, it can also detect some diseases in patients who may not have symptoms, preventing the spread of the virus. There are some types of CAD systems, such as Machine and Deep Learning-based and Transfer learning-based. This chapter proposes a pipeline for feature extraction and classification of Covid-19 in X-ray images using transfer learning for feature extraction with VGG-16 CNN and machine learning classifiers. Five classifiers were evaluated: Accuracy, Specificity, Sensitivity, Geometric mean, and Area under the curve. The SVM Classifier presented the best performance metrics for Covid-19 classification, achieving 90% accuracy, 97.5% of Specificity, 82.5% of Sensitivity, 89.6% of Geometric mean, and 90% for the AUC metric. On the other hand, the Nearest Centroid (NC) classifier presented poor sensitivity and geometric mean results, achieving 33.9% and 54.07%, respectively. CY - Cham DA - 2023/// PY - 2023 DP - Springer Link SP - 65 EP - 78 LA - en PB - Springer International Publishing SN - 978-3-031-30788-1 UR - https://doi.org/10.1007/978-3-031-30788-1_5 Y2 - 2023/10/10/04:37:07 ER - TY - CHAP TI - TPOT Automated Machine Learning Approach for Multiple Diagnostic Classification of Lung Radiography and Feature Extraction AU - Bernardo Gois, Francisco Nauber AU - Lobo Marques, Joao Alexandre AU - Fong, Simon James T2 - Computerized Systems for Diagnosis and Treatment of COVID-19 A2 - Lobo Marques, Joao Alexandre A2 - Fong, Simon James AB - This chapter describes an AUTO-ML strategy to detect COVID on chest X-rays utilizing Transfer Learning feature extraction and the AutoML TPOT framework in order to identify lung illnesses (such as COVID or pneumonia). MobileNet is a lightweight network that uses depthwise separable convolution to deepen the network while decreasing parameters and computation. AutoML is a revolutionary concept of automated machine learning (AML) that automates the process of building an ML pipeline inside a constrained computing framework. The term “AutoML” can mean a number of different things depending on context. AutoML has risen to prominence in both the business world and the academic community thanks to the ever-increasing capabilities of modern computers. Python Optimised ML Pipeline (TPOT) is a Python-based ML tool that optimizes pipeline efficiency via genetic programming. We use TPOT builds models for extracted MobileNet network features from COVID-19 image data. The f1-score of 0.79 classifies Normal, Viral Pneumonia, and Lung Opacity. CY - Cham DA - 2023/// PY - 2023 DP - Springer Link SP - 117 EP - 135 LA - en PB - Springer International Publishing SN - 978-3-031-30788-1 UR - https://doi.org/10.1007/978-3-031-30788-1_8 Y2 - 2023/10/10/04:37:12 ER - TY - CHAP TI - Technology Developments to Face the COVID-19 Pandemic: Advances, Challenges, and Trends AU - Lobo Marques, Joao Alexandre AU - Fong, Simon James T2 - Computerized Systems for Diagnosis and Treatment of COVID-19 A2 - Lobo Marques, Joao Alexandre A2 - Fong, Simon James AB - The global pandemic triggered by the Corona Virus Disease firstly detected in 2019 (COVID-19), entered the fourth year with many unknown aspects that need to be continuously studied by the medical and academic communities. According to the World Health Organization (WHO), until January 2023, more than 650 million cases were officially accounted (with probably much more non tested cases) with 6,656,601 deaths officially linked to the COVID-19 as plausible root cause. In this Chapter, an overview of some relevant technical aspects related to the COVID-19 pandemic is presented, divided in three parts. First, the advances are highlighted, including the development of new technologies in different areas such as medical devices, vaccines, and computerized system for medical support. Second, the focus is on relevant challenges, including the discussion on how computerized diagnostic supporting systems based on Artificial Intelligence are in fact ready to effectively help on clinical processes, from the perspective of the model proposed by NASA, Technology Readiness Levels (TRL). Finally, two trends are presented with increased necessity of computerized systems to deal with the Long Covid and the interest on Precision Medicine digital tools. Analyzing these three aspects (advances, challenges, and trends) may provide a broader understanding of the impact of the COVID-19 pandemic on the development of Computerized Diagnostic Support Systems. CY - Cham DA - 2023/// PY - 2023 DP - Springer Link SP - 1 EP - 13 LA - en PB - Springer International Publishing SN - 978-3-031-30788-1 ST - Technology Developments to Face the COVID-19 Pandemic UR - https://doi.org/10.1007/978-3-031-30788-1_1 Y2 - 2023/10/10/04:37:00 ER - TY - CHAP TI - Segmentation of CT-Scan Images Using UNet Network for Patients Diagnosed with COVID-19 AU - Bernardo Gois, Francisco Nauber AU - Lobo Marques, Joao Alexandre T2 - Computerized Systems for Diagnosis and Treatment of COVID-19 A2 - Lobo Marques, Joao Alexandre A2 - Fong, Simon James AB - The use of computational tools for medical image processing are promising tools to effectively detect COVID-19 as an alternative to expensive and time-consuming RT-PCR tests. For this specific task, CXR (Chest X-Ray) and CCT (Chest CT Scans) are the most common examinations to support diagnosis through radiology analysis. With these images, it is possible to support diagnosis and determine the disease’s severity stage. Computerized COVID-19 quantification and evaluation require an efficient segmentation process. Essential tasks for automatic segmentation tools are precisely identifying the lungs, lobes, bronchopulmonary segments, and infected regions or lesions. Segmented areas can provide handcrafted or self-learned diagnostic criteria for various applications. This Chapter presents different techniques applied for Chest CT Scans segmentation, considering the state of the art of UNet networks to segment COVID-19 CT scans and a segmentation experiment for network evaluation. Along 200 epochs, a dice coefficient of 0.83 was obtained. CY - Cham DA - 2023/// PY - 2023 DP - Springer Link SP - 29 EP - 44 LA - en PB - Springer International Publishing SN - 978-3-031-30788-1 UR - https://doi.org/10.1007/978-3-031-30788-1_3 Y2 - 2023/10/10/04:37:03 ER - TY - BOOK TI - Predictive Models for Decision Support in the COVID-19 Crisis AU - Marques, João Alexandre Lobo AU - Gois, Francisco Nauber Bernardo AU - Xavier-Neto, Jose AU - Fong, Simon James T2 - SpringerBriefs in Applied Sciences and Technology AB - COVID-19 has hit the world unprepared, as the deadliest pandemic of the century. Governments and authorities, as leaders and decision makers fighting the virus, enormously tap into the power of artificial intelligence and its predictive models for urgent decision support. This book showcases a collection of important predictive models that used during the pandemic, and discusses and compares their efficacy and limitations. Readers from both healthcare industries and academia can gain unique insights on how predictive models were designed and applied on epidemic data. Taking COVID19 as a case study and showcasing the lessons learnt, this book will enable readers to be better prepared in the event of virus epidemics or pandemics in the future. DA - 2021/// PY - 2021 LA - en PB - Springer International Publishing SN - 978-3-030-61912-1 UR - https://www.springer.com/gp/book/9783030619121 Y2 - 2021/01/29/07:53:53 ER - TY - CHAP TI - Lung Segmentation of Chest X-Rays Using Unet Convolutional Networks AU - dos Santos Silva, Bruno Riccelli AU - Cesar Cortez, Paulo AU - Gomes Aguiar, Rafael AU - Rodrigues Ribeiro, Tulio AU - Pereira Teixeira, Alexandre AU - Bernardo Gois, Francisco Nauber AU - Lobo Marques, Joao Alexandre T2 - Computerized Systems for Diagnosis and Treatment of COVID-19 A2 - Lobo Marques, Joao Alexandre A2 - Fong, Simon James AB - The gold standard to detect SARS-CoV-2 infection consider testing methods based on Polymerase Chain Reaction (PCR). Still, the time necessary to confirm patient infection can be lengthy, and the process is expensive. On the other hand, X-Ray and CT scans play a vital role in the auxiliary diagnosis process. Hence, a trusted automated technique for identifying and quantifying the infected lung regions would be advantageous. Chest X-rays are two-dimensional images of the patient’s chest and provide lung morphological information and other characteristics, like ground-glass opacities (GGO), horizontal linear opacities, or consolidations, which are characteristics of pneumonia caused by COVID-19. But before the computerized diagnostic support system can classify a medical image, a segmentation task should usually be performed to identify relevant areas to be analyzed and reduce the risk of noise and misinterpretation caused by other structures eventually present in the images. This chapter presents an AI-based system for lung segmentation in X-ray images using a U-net CNN model. The system’s performance was evaluated using metrics such as cross-entropy, dice coefficient, and Mean IoU on unseen data. Our study divided the data into training and evaluation sets using an 80/20 train-test split method. The training set was used to train the model, and the evaluation test set was used to evaluate the performance of the trained model. The results of the evaluation showed that the model achieved a Dice Similarity Coefficient (DSC) of 95%, Cross entropy of 97%, and Mean IoU of 86%. CY - Cham DA - 2023/// PY - 2023 DP - Springer Link SP - 15 EP - 28 LA - en PB - Springer International Publishing SN - 978-3-031-30788-1 UR - https://doi.org/10.1007/978-3-031-30788-1_2 Y2 - 2023/10/10/04:41:11 ER - TY - CHAP TI - Exploratory Data Analysis on Clinical and Emotional Parameters of Pregnant Women with COVID-19 Symptoms AU - Lobo Marques, Joao Alexandre AU - Macedo, Danielle S. AU - Motta, Pedro AU - dos Santos Silva, Bruno Riccelli AU - Carvalho, Francisco Herlanio Costa AU - Kehdi, Renata Castro AU - Cavalcante, Letícia Régia Lima AU - da Silva Viana, Marylane AU - Lós, Deniele AU - Fiorenza, Natália Gindri T2 - Computerized Systems for Diagnosis and Treatment of COVID-19 A2 - Lobo Marques, Joao Alexandre A2 - Fong, Simon James AB - The scientific literature indicates that pregnant women with COVID-19 are at an increased risk for developing more severe illness conditions when compared with non-pregnant women. The risk of admission to an ICU (Intensive Care Unit) and the need for mechanical ventilator support is three times higher. More significantly, statistics indicate that these patients are also at 70% increased risk of evolving to severe states or even death. In addition, other previous illnesses and age greater than 35 years old increase the risk for the mother and the fetus, including a higher number of cesarean sections, higher systolic and diastolic maternal blood pressure, increasing the risk of eclampsia, and, in some cases, preterm birth. Additionally, pregnant women have more Emotional lability/fluctuations (between positive and negative feelings) during the entire pregnancy. The emotional instability and brain fog that takes place during gestation may open vulnerability for neuropsychiatric symptoms of long COVID, which this population was not studied in depth. The present Chapter characterizes the database presented in this work with clinical and survey data collected about emotions and feelings using the Coronavirus Perinatal Experiences—Impact Survey (COPE-IS). Pregnant women with or without COVID-19 symptoms who gave birth at the Assis Chateaubriand Maternity Hospital (MEAC), a public maternity of the Federal University of Ceara, Brazil, were recruited. In total, 72 mother-infant dyads were included in the study and are considered in this exploratory analysis. The participants have undergone serological tests for SARS-CoV-2 antibody detection and a nasopharyngeal swab test for COVID-19 diagnoses by RT-PCR. A comprehensive Exploratory Data Analysis (EDA) is performed using frequency distribution analysis of multiple types of variables generated from numerical data, multiple-choice, categorized, and Likert-scale questions. CY - Cham DA - 2023/// PY - 2023 DP - Springer Link SP - 179 EP - 209 LA - en PB - Springer International Publishing SN - 978-3-031-30788-1 UR - https://doi.org/10.1007/978-3-031-30788-1_11 Y2 - 2023/10/10/04:37:22 ER - TY - CHAP TI - Evaluation of ECG Non-linear Features in Time-Frequency Domain for the Discrimination of COVID-19 Severity Stages AU - Ribeiro, Pedro AU - Pordeus, Daniel AU - Zacarias, Laíla AU - Leite, Camila AU - Alves Neto, Manoel AU - Aires Peixoto Jr, Arnaldo AU - de Oliveira, Adriel AU - Paulo Madeiro, João AU - Lobo Marques, Joao Alexandre AU - Miguel Rodrigues, Pedro T2 - Computerized Systems for Diagnosis and Treatment of COVID-19 A2 - Lobo Marques, Joao Alexandre A2 - Fong, Simon James AB - In 2020, the World Health Organization declared the Coronavirus Disease 19 a global pandemic. While detecting COVID-19 is essential in controlling the disease, prognosis prediction is crucial in reducing disease complications and patient mortality. For that, standard protocols consider adopting medical imaging tools to analyze cases of pneumonia and complications. Nevertheless, some patients develop different symptoms and/or cannot be moved to a CT-Scan room. In other cases, the devices are not available. The adoption of ambulatory monitoring examinations, such as Electrocardiography (ECG), can be considered a viable tool to address the patient’s cardiovascular condition and to act as a predictor for future disease outcomes. In this investigation, ten non-linear features (Energy, Approximate Entropy, Logarithmic Entropy, Shannon Entropy, Hurst Exponent, Lyapunov Exponent, Higuchi Fractal Dimension, Katz Fractal Dimension, Correlation Dimension and Detrended Fluctuation Analysis) extracted from 2 ECG signals (collected from 2 different patient’s positions). Windows of 1 second segments in 6 ways of windowing signal analysis crops were evaluated employing statistical analysis. Three categories of outcomes are considered for the patient status: Low, Moderate, and Severe, and four combinations for classification scenarios are tested:  (Low vs. Moderate, Low vs. Severe, Moderate vs. Severe) and 1 Multi-class comparison  (All vs. All)). The results indicate that some statistically significant parameter distributions were found for all comparisons.  (Low vs. Moderate—Approximate Entropy p-value = 0.0067 < 0.05, Low vs. Severe—Correlation Dimension p-value = 0.0087 < 0.05, Moderate vs. Severe—Correlation Dimension p-value = 0.0029 < 0.05, All vs. All—Correlation Dimension p-value = 0.0185 < 0.05. The non-linear analysis of the time-frequency representation of the ECG signal can be considered a promising tool for describing and distinguishing the COVID-19 severity activity along its different stages. CY - Cham DA - 2023/// PY - 2023 DP - Springer Link SP - 137 EP - 154 LA - en PB - Springer International Publishing SN - 978-3-031-30788-1 UR - https://doi.org/10.1007/978-3-031-30788-1_9 Y2 - 2023/10/10/04:41:02 KW - COVID-19 KW - ECG signals KW - Non-linear analysis KW - Statistical analysis ER - TY - CHAP TI - Covid-19 Detection Based on Chest X-Ray Images Using Multiple Transfer Learning CNN Models AU - dos Santos Silva, Bruno Riccelli AU - Cesar Cortez, Paulo AU - Crosara Motta, Pedro AU - Lobo Marques, Joao Alexandre T2 - Computerized Systems for Diagnosis and Treatment of COVID-19 A2 - Lobo Marques, Joao Alexandre A2 - Fong, Simon James AB - The gold standard to detect SARS-CoV-2 infection considers testing methods based on Polymerase Chain Reaction (PCR). Still, the time necessary to confirm patient infection can be lengthy, and the process is expensive. In parallel, X-Ray and CT scans play an important role in the diagnosis and treatment processes. Hence, a trusted automated technique for identifying and quantifying the infected lung regions would be advantageous. Chest X-rays are two-dimensional images of the patient’s chest and provide lung morphological information and other characteristics, like ground-glass opacities (GGO), horizontal linear opacities, or consolidations, which are typical characteristics of pneumonia caused by COVID-19. This chapter presents an AI-based system using multiple Transfer Learning models for COVID-19 classification using Chest X-Rays. In our experimental design, all the classifiers demonstrated satisfactory accuracy, precision, recall, and specificity performance. On the one hand, the Mobilenet architecture outperformed the other CNNs, achieving excellent results for the evaluated metrics. On the other hand, Squeezenet presented a regular result in terms of recall. In medical diagnosis, false negatives can be particularly harmful because a false negative can lead to patients being incorrectly diagnosed as healthy. These results suggest that our Deep Learning classifiers can accurately classify X-ray exams as normal or indicative of COVID-19 with high confidence. CY - Cham DA - 2023/// PY - 2023 DP - Springer Link SP - 45 EP - 63 LA - en PB - Springer International Publishing SN - 978-3-031-30788-1 UR - https://doi.org/10.1007/978-3-031-30788-1_4 Y2 - 2023/10/10/04:37:05 ER - TY - CHAP TI - COVID-19 Classification Using CT Scans with Convolutional Neural Networks AU - Motta, Pedro Crosara AU - Cesar Cortez, Paulo AU - Lobo Marques, Jao Alexandre T2 - Computerized Systems for Diagnosis and Treatment of COVID-19 A2 - Lobo Marques, Joao Alexandre A2 - Fong, Simon James AB - Even with more than 12 billion vaccine doses administered globally, the Covid-19 pandemic has caused several global economic, social, environmental, and healthcare impacts. Computer Aided Diagnostic (CAD) systems can serve as a complementary method to aid doctors in identifying regions of interest in images and help detect diseases. In addition, these systems can help doctors analyze the status of the disease and check for their progress or regression. To analyze the viability of using CNNs for differentiating Covid-19 CT positive images from Covid-19 CT negative images, we used a dataset collected by Union Hospital (HUST-UH) and Liyuan Hospital (HUST-LH) and made available at the Kaggle platform. The main objective of this chapter is to present results from applying two state-of-the-art CNNs on a Covid-19 CT Scan images database to evaluate the possibility of differentiating images with imaging features associated with Covid-19 pneumonia from images with imaging features irrelevant to Covid-19 pneumonia. Two pre-trained neural networks, ResNet50 and MobileNet, were fine-tuned for the datasets under analysis. Both CNNs obtained promising results, with the ResNet50 network achieving a Precision of 0.97, a Recall of 0.96, an F1-score of 0.96, and 39 false negatives. The MobileNet classifier obtained a Precision of 0.94, a Recall of 0.94, an F1-score of 0.94, and a total of 20 false negatives. CY - Cham DA - 2023/// PY - 2023 DP - Springer Link SP - 99 EP - 116 LA - en PB - Springer International Publishing SN - 978-3-031-30788-1 UR - https://doi.org/10.1007/978-3-031-30788-1_7 Y2 - 2023/10/11/10:42:48 ER - TY - BOOK TI - Computerized Systems for Diagnosis and Treatment of COVID-19 A3 - Lobo Marques, Joao Alexandre A3 - Fong, Simon James CY - Cham DA - 2023/// PY - 2023 DP - DOI.org (Crossref) LA - en PB - Springer International Publishing SN - 978-3-031-30787-4 978-3-031-30788-1 UR - https://link.springer.com/10.1007/978-3-031-30788-1 Y2 - 2023/10/10/04:35:42 KW - Artificial Intelligence KW - Biofeedback KW - Computerized Diagnostic Support KW - Covid-19 KW - Signal and Image Processing ER - TY - CHAP TI - Classification of Severity of COVID-19 Patients Based on the Heart Rate Variability AU - Pordeus, Daniel AU - Ribeiro, Pedro AU - Zacarias, Laíla AU - Paulo Madeiro, João AU - Lobo Marques, Joao Alexandre AU - Miguel Rodrigues, Pedro AU - Leite, Camila AU - Alves Neto, Manoel AU - Aires Peixoto Jr, Arnaldo AU - de Oliveira, Adriel T2 - Computerized Systems for Diagnosis and Treatment of COVID-19 A2 - Lobo Marques, Joao Alexandre A2 - Fong, Simon James AB - The continuous development of robust machine learning algorithms in recent years has helped to improve the solutions of many studies in many fields of medicine, rapid diagnosis and detection of high-risk patients with poor prognosis as the coronavirus disease 2019 (COVID-19) spreads globally, and also early prevention of patients and optimization of medical resources. Here, we propose a fully automated machine learning system to classify the severity of COVID-19 from electrocardiogram (ECG) signals. We retrospectively collected 100 5-minute ECGs from 50 patients in two different positions, upright and supine. We processed the surface ECG to obtain QRS complexes and HRV indices for RR series, including a total of 43 features. We compared 19 machine learning classification algorithms that yielded different approaches explained in a methodology session. CY - Cham DA - 2023/// PY - 2023 DP - Springer Link SP - 155 EP - 177 LA - en PB - Springer International Publishing SN - 978-3-031-30788-1 UR - https://doi.org/10.1007/978-3-031-30788-1_10 Y2 - 2023/10/10/04:37:17 KW - COVID-19 KW - Electrocardiogram (ECG) signal KW - Heart Rate Variability (HRV) indices KW - Severity KW - Signal processing ER - TY - CHAP TI - Classification of COVID-19 CT Scans Using Convolutional Neural Networks and Transformers AU - Bernardo Gois, Francisco Nauber AU - Lobo Marques, Joao Alexandre AU - Fong, Simon James T2 - Computerized Systems for Diagnosis and Treatment of COVID-19 A2 - Lobo Marques, Joao Alexandre A2 - Fong, Simon James AB - COVID-19 is a respiratory disorder caused by CoronaVirus and SARS (SARS-CoV2). WHO declared COVID-19 a global pandemic in March 2020 and several nations’ healthcare systems were on the verge of collapsing. With that, became crucial to screen COVID-19-positive patients to maximize limited resources. NAATs and antigen tests are utilized to diagnose COVID-19 infections. NAATs reliably detect SARS-CoV-2 and seldom produce false-negative results. Because of its specificity and sensitivity, RT-PCR can be considered the gold standard for COVID-19 diagnosis. This test’s complex gear is pricey and time-consuming, using skilled specialists to collect throat or nasal mucus samples. These tests require laboratory facilities and a machine for detection and analysis. Deep learning networks have been used for feature extraction and classification of Chest CT-Scan images and as an innovative detection approach in clinical practice. Because of COVID-19 CT scans’ medical characteristics, the lesions are widely spread and display a range of local aspects. Using deep learning to diagnose directly is difficult. In COVID-19, a Transformer and Convolutional Neural Network module are presented to extract local and global information from CT images. This chapter explains transfer learning, considering VGG-16 network, in CT examinations and compares convolutional networks with Vision Transformers (ViT). Vit usage increased VGG-16 network F1-score to 0.94. CY - Cham DA - 2023/// PY - 2023 DP - Springer Link SP - 79 EP - 97 LA - en PB - Springer International Publishing SN - 978-3-031-30788-1 UR - https://doi.org/10.1007/978-3-031-30788-1_6 Y2 - 2023/10/10/04:37:10 ER - TY - JOUR TI - A review on multimodal machine learning in medical diagnostics AU - Yan, Keyue AU - Li, Tengyue AU - Marques, João Alexandre Lobo AU - Gao, Juntao AU - Fong, Simon James AU - Yan, Keyue AU - Li, Tengyue AU - Marques, João Alexandre Lobo AU - Gao, Juntao AU - Fong, Simon James T2 - Mathematical Biosciences and Engineering AB - Nowadays, the increasing number of medical diagnostic data and clinical data provide more complementary references for doctors to make diagnosis to patients. For example, with medical data, such as electrocardiography (ECG), machine learning algorithms can be used to identify and diagnose heart disease to reduce the workload of doctors. However, ECG data is always exposed to various kinds of noise and interference in reality, and medical diagnostics only based on one-dimensional ECG data is not trustable enough. By extracting new features from other types of medical data, we can implement enhanced recognition methods, called multimodal learning. Multimodal learning helps models to process data from a range of different sources, eliminate the requirement for training each single learning modality, and improve the robustness of models with the diversity of data. Growing number of articles in recent years have been devoted to investigating how to extract data from different sources and build accurate multimodal machine learning models, or deep learning models for medical diagnostics. This paper reviews and summarizes several recent papers that dealing with multimodal machine learning in disease detection, and identify topics for future research. DA - 2023/// PY - 2023 DO - 10.3934/mbe.2023382 DP - www.aimspress.com VL - 20 IS - 5 SP - 8708 EP - 8726 J2 - MBE LA - en SN - 1551-0018 UR - http://www.aimspress.com/rticle/doi/10.3934/mbe.2023382 Y2 - 2023/03/21/16:22:08 ER -