TY - JOUR TI - Effects of a High-Fat Diet on Intestinal and Gonadal Metabolism in Female and Male Sea Cucumber Apostichopus japonicus AU - Zhang, Shuangyan AU - Ru, Xiaoshang AU - Zhang, Libin AU - Gonçalves, David AU - Yang, Hongsheng AU - Xu, Jialei T2 - Biology AB - Parental nutrient reserves are directly related to reproductive performance in sea cucumbers. This study focused on the lipid requirements of male and female sea cucumbers Apostichopus japonicus during the reproductive stage and analyzed their physiological responses to a high-fat diet (HFD). The intestinal lipid metabolites and microbiome profile changed significantly in animals fed with the HFD, as given by an upregulation of metabolites related to lipid metabolism and an increase in the predominance of Proteobacteria in the microbiome, respectively. The metabolic responses of male and female sea cucumbers to the HFD differed, which in turn could have triggered sex-related differences in the intestinal microbiome. These results suggest that the lipid content in diets can be differentially adjusted for male and female sea cucumbers to improve nutrition and promote reproduction. This data contributes to a better understanding of the reproductive biology and sex differences of sea cucumbers. DA - 2023/02// PY - 2023 DO - 10.3390/biology12020212 DP - www.mdpi.com VL - 12 IS - 2 SP - 212 LA - en SN - 2079-7737 UR - https://www.mdpi.com/2079-7737/12/2/212 Y2 - 2023/04/11/09:54:51 KW - high-fat diet KW - intestinal microbes KW - physiological metabolic responses KW - sea cucumber KW - sex differences ER - TY - JOUR TI - Examination of sex-related differences in intestinal and gonadal lipid metabolism in the sea cucumber Apostichopus japonicus AU - Zhang, Shuangyan AU - Ru, Xiaoshang AU - Zhang, Libin AU - Gonçalves, David AU - Yang, Hongsheng T2 - Aquaculture AB - Reproduction of the sea cucumber Apostichopus japonicus is critical for aquaculture production. Gonadal development is the basis of reproduction, and lipids, which are among the main nutrients required for gonadal development, directly affect reproduction. We investigated whether gonadal and intestinal lipid metabolism differed between male and female A. japonicus. Transcriptome analysis of the intestines of sexually mature male and female wild-caught individuals revealed differences in gene expression, with 27 and 39 genes being up-regulated in females and males, respectively. In particular, the expression of the fatty acid synthase gene was higher in males than in females. Metabolome analysis of the gonads identified 141 metabolites that were up-regulated and 175 metabolites that were down-regulated in the testes compared with the ovaries in the positive/negative mode of an LC-MS/MS analysis. A variety of polyunsaturated fatty acids were found at higher concentrations in the testes than in the ovaries. 16 s rDNA sequencing analysis showed that the composition and structure of the intestinal microbiota were similar between males and females. These results suggest that sex differences in intestinal metabolism of A. japonicus are not due to differences in the microbiota, and we speculate that gonadal metabolism may be related to intestinal morphology. This information might be useful in improving the reproductive efficiency of sea cucumbers in captivity. DA - 2023/01/15/ PY - 2023 DO - 10.1016/j.aquaculture.2022.738787 DP - ScienceDirect VL - 562 SP - 738787 J2 - Aquaculture LA - en SN - 0044-8486 UR - https://www.sciencedirect.com/science/article/pii/S0044848622009048 Y2 - 2023/04/11/10:01:48 KW - Gonads KW - Intestines KW - Lipid metabolism KW - Sea cucumber KW - Sex differences ER - TY - JOUR TI - Physiology, endocrinology and chemical communication in aggressive behaviour of fishes AU - Silva, Melina Coelho da AU - Canário, Adelino Vicente Mendonça AU - Hubbard, Peter Colin AU - Gonçalves, David T2 - Journal of Fish Biology AB - Fishes show remarkably diverse aggressive behaviour. Aggression is expressed to secure resources; adjusting aggression levels according to context is key to avoid negative consequences for fitness and survival. Nonetheless, despite its importance, the physiological basis of aggression in fishes is still poorly understood. Several reports suggest hormonal modulation of aggression, particularly by androgens, but contradictory studies have been published. Studies exploring the role of chemical communication in aggressive behaviour are also scant, and the pheromones involved remain to be unequivocally characterized. This is surprising as chemical communication is the most ancient form of information exchange and plays a variety of other roles in fishes. Furthermore, the study of chemical communication and aggression is relevant at the evolutionary, ecological and economic levels. A few pioneering studies support the hypothesis that aggressive behaviour, at least in some teleosts, is modulated by “dominance pheromones” that reflect the social status of the sender, but there is little information on the identity of the compounds involved. This review aims to provide a global view of aggressive behaviour in fishes and its underlying physiological mechanisms including the involvement of chemical communication, and discusses the potential use of dominance pheromones to improve fish welfare. Methodological considerations and future research directions are also outlined. DA - 2021/// PY - 2021 DO - 10.1111/jfb.14667 J2 - J Fish Biol LA - en SN - 0022-1112, 1095-8649 UR - https://onlinelibrary.wiley.com/doi/10.1111/jfb.14667 Y2 - 2021/01/26/08:12:46 ER - TY - JOUR TI - Interpopulational variation of the mating system in the peacock blenny Salaria pavo AU - Saraiva, João L. AU - Pignolo, Giulia AU - Gonçalves, David AU - Oliveira, Rui F. T2 - acta ethologica DA - 2012/04// PY - 2012 DO - 10.1007/s10211-011-0104-y DP - DOI.org (Crossref) VL - 15 IS - 1 SP - 25 EP - 31 J2 - acta ethol LA - en SN - 0873-9749, 1437-9546 UR - http://link.springer.com/10.1007/s10211-011-0104-y Y2 - 2021/02/19/09:00:17 ER - TY - JOUR TI - Ecological modulation of reproductive behaviour in the peacock blenny: a mini-review AU - Saraiva, J. L. AU - Gonçalves, David AU - Oliveira, R. F. T2 - Fish Physiology and Biochemistry DA - 2013/02// PY - 2013 DO - 10.1007/s10695-012-9658-5 VL - 39 IS - 1 SP - 85 EP - 89 J2 - Fish Physiol Biochem LA - en SN - 0920-1742, 1573-5168 ST - Ecological modulation of reproductive behaviour in the peacock blenny UR - http://link.springer.com/10.1007/s10695-012-9658-5 Y2 - 2021/02/19/08:52:59 ER - TY - JOUR TI - Androgens and corticosteroids increase in response to mirror images and interacting conspecifics in males of the Siamese fighting fish Betta splendens AU - Ramos, Andreia AU - Alex, Deepa AU - Cardoso, Sara D. AU - Gonçalves, David T2 - Hormones and Behavior AB - The role of hormones as modulators of aggressive behavior in fish remains poorly understood. Androgens and corticosteroids, in particular, have been associated with aggressive behavior in fish but it is still not clear if animals adjust the secretion of these hormones to regulate behavior during ongoing fights, in response to fight outcomes in order to adjust aggressive behavior in subsequent fights, or both. With its stereotyped displays and high aggression levels, the Siamese fighting fish Betta splendens is an excellent model to investigate this question. Here, we compared the behavioral and endocrine response of male B. splendens to fights where there is no winner or loser by presenting them with a size-matched live interacting conspecific behind a transparent partition or with a mirror image. The aggressive response started with threat displays that were overall similar in frequency and duration towards both types of stimuli. Fights transitioned to overt attacks and interacting with a live conspecific elicited a higher frequency of attempted bites and head hits, as compared with the mirror image. There was a pronounced increase in plasma androgens (11-ketotestosterone and testosterone) and corticosteroids (cortisol) levels in response to the aggression challenge, independent of stimulus type. Post-fight intra-group levels of these hormones did not correlate with measures of physical activity or aggressive behavior. A linear discriminant analysis including all behavioral and endocrine data was a poor classifier of fish from the conspecific and mirror trials, showing that overall the behavioral and endocrine response to mirror images and conspecifics was similar. The results show that fight resolution is not necessary to induce an evident increase in peripheral levels of androgens and corticosteroids in B. splendens. However, the function of these hormones during present and future aggressive contests remains to be clarified. DA - 2021/06/01/ PY - 2021 DO - 10.1016/j.yhbeh.2021.104991 DP - ScienceDirect VL - 132 SP - 104991 J2 - Hormones and Behavior LA - en SN - 0018-506X UR - https://www.sciencedirect.com/science/article/pii/S0018506X21000702 Y2 - 2023/04/11/13:53:24 KW - Aggression KW - Androgens KW - Corticosteroids KW - Live conspecific test KW - Mirror test ER - TY - JOUR TI - Artificial selection for male winners in the Siamese fighting fish Betta splendens correlates with high female aggression AU - Ramos, A. AU - Gonçalves, D. T2 - Frontiers in Zoology AB - In Southeast Asia, males of the Siamese fighting fish Betta splendens have been selected across centuries for paired-staged fights. During the selection process, matched for size males fight in a small tank until the contest is resolved. Breeders discard losing batches and reproduce winner batches with the aim of increasing fight performance. We assessed the results of this long-term selection process by comparing under standard laboratory conditions male and female aggressive behaviour of one strain selected for staged fights (“fighters”) and one strain of wild-types. The aggressive response of adult fish was tested against their mirror image or a size-matched conspecific. Fighter males were more aggressive than wild-type males for all measured behaviours. Differences were not only quantitative but the pattern of fight display was also divergent. Fighter males had an overall higher swimming activity, performing frequent fast strikes in the direction of the intruder and displaying from a distance. Wild-type males were less active and exhibited aggressive displays mostly in close proximity to the stimuli. Females of the fighter strain, which are not used for fights, were also more aggressive than wild-type females. Aggressive behaviours were correlated across male and female fighter siblings, suggesting common genetic and physiological mechanisms to male and female aggression in this species. The study further shows that results were largely independent of the stimulus type, with the mirror test inducing similar and less variable responses than the live conspecific presentation. These results suggest that selection for male winners co-selected for high-frequency and metabolic demanding aggressive display in males and also enhanced female aggression, opening a wide range of testable hypothesis about the ultimate and proximate mechanisms of male and female aggression in B. splendens. DA - 2019/08/08/ PY - 2019 DO - 10.1186/s12983-019-0333-x DP - BioMed Central VL - 16 IS - 1 SP - 34 J2 - Frontiers in Zoology SN - 1742-9994 UR - https://doi.org/10.1186/s12983-019-0333-x Y2 - 2023/04/11/14:17:52 KW - Aggression KW - Artificial selection KW - Domestication KW - Mirror test KW - Sexual conflict ER - TY - JOUR TI - Selection for winners impacts the endocrine system in the Siamese fighting fish AU - Ramos, A. AU - Gonçalves, D. T2 - General and Comparative Endocrinology AB - In southeast Asia, males of the Siamese fighting fish, Betta splendens, have been selected across centuries for winning paired staged fights and previous work has shown that males from fighter strains are more aggressive than wild-types. This strong directional selection for winners is likely to have targeted aggression-related endocrine systems, and a comparison between fighter and wild-type strains can bring into evidence the key hormones implicated in aggression. Here, we compared the plasma levels of the androgen 11-ketotestosterone (KT) and of the corticosteroid cortisol (F) in F2 males of a fighter and a wild-type strain raised under similar laboratory conditions. We show that F was generally lower in fighter as compared with wild-type males, while no overall differences in KT levels were detected between strains. When presented with a mirror-induced aggressive challenge, post-fight levels of F increased but more significantly so in wild-type males, while KT increased in males of both strains. After the challenge, fighter males had higher levels of KT as compared with wild-type males, while the pattern for F was opposite. As compared with animals in social groups, wild-type males placed under social isolation had lower F levels, while KT decreased for fighters. Taken together, this data suggests that while wild-type males responded to aggression with an increase in circulating levels of both androgens and corticosteroids, males selected for winning fights maintained a blunt F response, increasing only KT levels. These data agree with the hypothesis that a combination of high levels of androgens and low levels of corticosteroids is associated with high aggression. Overall, these results seem to indicate that selection for winning had a stronger impact in the hypothalamus-pituitary-interrenal axis than in the hypothalamus-pituitary–gonadal axis in B. splendens. DA - 2022/03// PY - 2022 DO - 10.1016/j.ygcen.2022.113988 DP - DOI.org (Crossref) VL - 318 SP - 113988 J2 - General and Comparative Endocrinology LA - en SN - 00166480 UR - https://linkinghub.elsevier.com/retrieve/pii/S0016648022000132 Y2 - 2023/04/11/10:06:18 ER - TY - JOUR TI - Plasticity in reproductive behaviour in two populations of the peacock blenny AU - Oliveira, Rui F. AU - Simões, Mariana G. AU - Gonçalves, David AU - Saraiva, João L. T2 - Behaviour DA - 2011/// PY - 2011 DO - 10.1163/156853911X617089 DP - DOI.org (Crossref) VL - 148 IS - 14 SP - 1457 EP - 1472 J2 - Behav SN - 0005-7959, 1568-539X UR - https://brill.com/view/journals/beh/148/14/article-p1457_3.xml Y2 - 2021/02/19/09:02:52 ER - TY - JOUR TI - Sediments in the mangrove areas contribute to the removal of endocrine disrupting chemicals in coastal sediments of Macau SAR, China, and harbour microbial communities capable of degrading E2, EE2, BPA and BPS AU - Moreira, Irina S. AU - Lebel, Alexandre AU - Peng, Xianzhi AU - Castro, Paula M. L. AU - Gonçalves, David T2 - Biodegradation AB - The occurrence of endocrine disrupting chemicals (EDCs) is a major issue for marine and coastal environments in the proximity of urban areas. The occurrence of EDCs in the Pearl River Delta region is well documented but specific data related to Macao is unavailable. The levels of bisphenol-A (BPA), estrone (E1), 17α-estradiol (αE2), 17β-estradiol (E2), estriol (E3), and 17α-ethynylestradiol (EE2) were measured in sediment samples collected along the coastline of Macao. BPA was found in all 45 collected samples with lower BPA concentrations associated to the presence of mangrove trees. Biodegradation assays were performed to evaluate the capacity of the microbial communities of the surveyed ecosystems to degrade BPA and its analogue BPS. Using sediments collected at a WWTP discharge point as inoculum, at a concentration of 2 mg l−1 complete removal of BPA was observed within 6 days, whereas for the same concentration BPS removal was of 95% after 10 days, which is particularly interesting since this compound is considered recalcitrant to biodegradation and likely to accumulate in the environment. Supplementation with BPA improved the degradation of bisphenol-S (BPS). Aiming at the isolation of EDCs-degrading bacteria, enrichments were established with sediments supplied with BPA, BPS, E2 and EE2, which led to the isolation of a bacterial strain, identified as Rhodoccoccus sp. ED55, able to degrade the four compounds at different extents. The isolated strain represents a valuable candidate for bioremediation of contaminated soils and waters. DA - 2021/10/01/ PY - 2021 DO - 10.1007/s10532-021-09948-9 DP - Springer Link VL - 32 IS - 5 SP - 511 EP - 529 J2 - Biodegradation LA - en SN - 1572-9729 UR - https://doi.org/10.1007/s10532-021-09948-9 Y2 - 2023/04/11/13:52:56 KW - Biodegradation KW - Bisphenols KW - EDCs-degrading bacteria KW - Endocrine disrupting chemicals (EDCs) KW - Estrogens KW - Mangrove ER - TY - JOUR TI - Macao air quality forecast using statistical methods AU - Lei, Man Tat AU - Monjardino, Joana AU - Mendes, Luisa AU - Gonçalves, David AU - Ferreira, Francisco T2 - Air Quality, Atmosphere & Health AB - The levels of air pollution in Macao often exceeded the levels recommended by WHO. In order for the population to take precautionary measures and avoid further health risks under high pollutant exposure, it is important to develop a reliable air quality forecast. Statistical models based on linear multiple regression (MR) and classification and regression trees (CART) analysis were developed successfully, for Macao, to predict the next day concentrations of NO2, PM10, PM2.5, and O3. All the developed models were statistically significantly valid with a 95% confidence level with high coefficients of determination (from 0.78 to 0.93) for all pollutants. The models utilized meteorological and air quality variables based on 5 years of historical data, from 2013 to 2017. Data from 2013 to 2016 were used to develop the statistical models and data from 2017 was used for validation purposes. A wide range of meteorological and air quality variables was identified, and only some were selected as significant independent variables. Meteorological variables were selected from an extensive list of variables, including geopotential height, relative humidity, atmospheric stability, and air temperature at different vertical levels. Air quality variables translate the resilience of the recent past concentrations of each pollutant and usually are maximum and/or the average of latest 24-h levels. The models were applied in forecasting the next day average daily concentrations for NO2 and PM and maximum hourly O3 levels for five air quality monitoring stations. The results are expected to be an operational air quality forecast for Macao. DA - 2019/09/01/ PY - 2019 DO - 10.1007/s11869-019-00721-9 DP - Springer Link VL - 12 IS - 9 SP - 1049 EP - 1057 J2 - Air Qual Atmos Health LA - en SN - 1873-9326 UR - https://doi.org/10.1007/s11869-019-00721-9 Y2 - 2022/09/21/08:23:38 KW - Macao KW - NO2 KW - O3 KW - PM10 KW - PM2.5 KW - Particulate matter ER - TY - JOUR TI - Statistical Forecast of Pollution Episodes in Macao during National Holiday and COVID-19 AU - Lei, Man Tat AU - Monjardino, Joana AU - Mendes, Luisa AU - Gonçalves, David AU - Ferreira, Francisco T2 - International Journal of Environmental Research and Public Health AB - Statistical methods such as multiple linear regression (MLR) and classification and regression tree (CART) analysis were used to build prediction models for the levels of pollutant concentrations in Macao using meteorological and air quality historical data to three periods: (i) from 2013 to 2016, (ii) from 2015 to 2018, and (iii) from 2013 to 2018. The variables retained by the models were identical for nitrogen dioxide (NO2), particulate matter (PM10), PM2.5, but not for ozone (O3) Air pollution data from 2019 was used for validation purposes. The model for the 2013 to 2018 period was the one that performed best in prediction of the next-day concentrations levels in 2019, with high coefficient of determination (R2), between predicted and observed daily average concentrations (between 0.78 and 0.89 for all pollutants), and low root mean square error (RMSE), mean absolute error (MAE), and biases (BIAS). To understand if the prediction model was robust to extreme variations in pollutants concentration, a test was performed under the circumstances of a high pollution episode for PM2.5 and O3 during 2019, and the low pollution episode during the period of implementation of the preventive measures for COVID-19 pandemic. Regarding the high pollution episode, the period of the Chinese National Holiday of 2019 was selected, in which high concentration levels were identified for PM2.5 and O3, with peaks of daily concentration exceeding 55 μg/m3 and 400 μg/m3, respectively. The 2013 to 2018 model successfully predicted this high pollution episode with high coefficients of determination (of 0.92 for PM2.5 and 0.82 for O3). The low pollution episode for PM2.5 and O3 was identified during the 2020 COVID-19 pandemic period, with a low record of daily concentration for PM2.5 levels at 2 μg/m3 and O3 levels at 50 μg/m3, respectively. The 2013 to 2018 model successfully predicted the low pollution episode for PM2.5 and O3 with a high coefficient of determination (0.86 and 0.84, respectively). Overall, the results demonstrate that the statistical forecast model is robust and able to correctly reproduce extreme air pollution events of both high and low concentration levels. DA - 2020/01// PY - 2020 DO - 10.3390/ijerph17145124 DP - www.mdpi.com VL - 17 IS - 14 SP - 5124 LA - en SN - 1660-4601 UR - https://www.mdpi.com/1660-4601/17/14/5124 Y2 - 2022/09/21/08:23:35 KW - COVID-19 KW - air pollution KW - air quality forecast KW - modelling KW - national holiday KW - pollution episodes ER - TY - CONF TI - Statistical Methods to Forecast Air Quality in Taipa Ambient and Taipa Residential of Macao AU - Lei, Man Tat AU - Monjardino, Joana AU - Mendes, Luisa AU - Gonçalves, David AU - Ferreira, Francisco A2 - Mensink, Clemens A2 - Matthias, Volker T3 - Springer Proceedings in Complexity AB - Air pollution is a major concern issue on Macao since the concentration levels of several of the most common pollutants are frequently above the internationally recommended values. The low air quality episodes impacts on human health paired with highly populated urban areas are important motivations to develop forecast methodologies in order to anticipate pollution episodes, allowing establishing warnings to the local community to take precautionary measures and avoid outdoor activities during this period. Using statistical methods (multiple linear regression (MLR) and classification and regression tree (CART) analysis) we were able to develop forecasting models for the main pollutants (NO2, PM2.5, and O3) enabling us to know the next day concentrations with a good skill, translated by high coefficients of determination (0.82–0.90) on a 95% confidence level. The model development was based on six years of historical data, 2013 to 2018, consisting of surface and upper-air meteorological observations and surface air quality observations. The year of 2019 was used for model validation. From an initially large group of meteorological and air quality variables only a few were identified as significant dependent variables in the model. The selected meteorological variables included geopotential height, relative humidity and air temperature at different altitude levels and atmospheric stability characterization parameters. The air quality predictors used included recent past hourly levels of mean concentrations for NO2 and PM2.5 and maximum concentrations for O3. The application of the obtained models provides the expected daily mean concentrations for NO2 and PM2.5 and maximum hourly concentrations O3 for the next day in Taipa Ambient air quality monitoring stations. The described methodology is now operational, in Macao, since 2020. C1 - Berlin, Heidelberg C3 - Air Pollution Modeling and its Application XXVII DA - 2021/// PY - 2021 DO - 10.1007/978-3-662-63760-9_25 DP - Springer Link SP - 167 EP - 173 LA - en PB - Springer SN - 978-3-662-63760-9 ER - TY - JOUR TI - Statistical Forecast Applied to Two Macao Air Monitoring Stations AU - Lei, M. AU - Monjardino, J. AU - Mendes, L. AU - Gonçalves, D. AU - Ferreira, F. T2 - IOP Conference Series: Earth and Environmental Science AB - The levels of air pollution in Macao often exceeded the levels recommended by WHO. In order for the population to take precautionary measures and avoid further health risks under high pollutant exposure, it is important to develop a reliable air quality forecast. Statistical models based on multiple regression (MR) analysis were developed successfully for Macao to predict the next day concentrations of PM10, PM2.5, and NO2. All the developed models were statistically significantly valid with a 95% confidence level with high coefficients of determination (from 0.89 to 0.92) for all pollutants. The models utilized meteorological and air quality variables based on five years of historical data, from 2013 to 2017. The data from 2013 to 2016 were used to develop the statistical models and data from 2017 were used for validation purposes. A wide range of meteorological and air quality variables were identified, and only some were selected as significant dependent variables. Meteorological variables were selected from an extensive list of variables, including geopotential height, relative humidity, atmospheric stability, and air temperature at different vertical levels. Air quality variables translate the resilience of the recent past concentrations of each pollutant and usually are maximum and/or the average of latest 24-hour levels. The models were applied in forecasting the next day average daily concentrations for PM10, PM2.5, and NO2 for the air quality monitoring stations. The results are expected to be an operational air quality forecast for Macao. DA - 2020/04// PY - 2020 DO - 10.1088/1755-1315/489/1/012018 DP - Institute of Physics VL - 489 IS - 1 SP - 012018 J2 - IOP Conf. Ser.: Earth Environ. Sci. LA - en SN - 1755-1315 UR - https://doi.org/10.1088/1755-1315/489/1/012018 Y2 - 2022/09/21/08:23:33 ER - TY - CHAP TI - Hormones and Sexual Behavior of Teleost Fishes AU - Gonçalves, David AU - Oliveira, Rui F. T2 - Hormones and Reproduction of Vertebrates DA - 2011/// PY - 2011 DP - DOI.org (Crossref) SP - 119 EP - 147 LA - en PB - Elsevier SN - 978-0-12-375009-9 UR - https://linkinghub.elsevier.com/retrieve/pii/B9780123750099100074 Y2 - 2021/02/19/09:02:07 ER - TY - CHAP TI - Neuroendocrinology of Social Behavior in Teleost Fish AU - Gonçalves, David AU - Félix, Ana S. AU - Oliveira, Rui F. T2 - Hormones, Brain and Behavior DA - 2017/// PY - 2017 SP - 3 EP - 18 LA - en PB - Elsevier SN - 978-0-12-803608-2 UR - https://linkinghub.elsevier.com/retrieve/pii/B9780128035924000201 Y2 - 2021/02/19/08:50:32 ER - TY - RPRT TI - Behavioral plasticity meets neuroplasticity: brain neurogenesis in the polymorphic fish Salaria pavo AU - Gonçalves, David Manuel Flores CY - Macao DA - 2016/// PY - 2016 M3 - Research Report PB - University of Saint Joseph ER - TY - JOUR TI - Alternative pathway history in a fish with plastic reproductive tactic AU - Gonçalves, David T2 - Functional Ecology DA - 2014/// PY - 2014 ER - TY - RPRT TI - FIGHT – Hormonal, pheromonal and genomic regulation of aggression in a fish model AU - Gonçalves, David CY - Macao DA - 2018/// PY - 2018 M3 - Research Report PB - University of Saint Joseph ER - TY - JOUR TI - Oestradiol and prostaglandin F2α regulate sexual display in female of a sex-role reversed fish AU - Gonçalves, David DA - 2014/// PY - 2014 VL - 281 IS - 1778 ER - TY - JOUR TI - Chromosome-level reference genome of the Siamese fighting fish Betta splendens, a model species for the study of aggression AU - Fan, Guangyi AU - Chan, Judy AU - Ma, Kailong AU - Yang, Binrui AU - Zhang, He AU - Yang, Xianwei AU - Shi, Chengcheng AU - Law, Henry AU - Ren, Zhitao AU - Xu, Qiwu AU - Liu, Qun AU - Wang, Jiahao AU - Chen, Wenbin AU - Shao, Libin AU - Gonçalves, David AU - Ramos, Andreia AU - Cardoso, Sara D AU - Guo, Min AU - Cai, Jing AU - Xu, Xun AU - Wang, Jian AU - Yang, Huanming AU - Liu, Xin AU - Wang, Yitao T2 - GigaScience DA - 2018/07/11/ PY - 2018 DO - 10.1093/gigascience/giy087 LA - en SN - 2047-217X UR - https://academic.oup.com/gigascience/advance-article/doi/10.1093/gigascience/giy087/5054042 Y2 - 2021/02/19/08:48:24 ER - TY - JOUR TI - Birth date predicts alternative life‐history pathways in a fish with sequential reproductive tactics AU - Fagundes, Teresa AU - Simões, Mariana G. AU - Saraiva, João L. AU - Ros, Albert F. H. AU - Gonçalves, David AU - Oliveira, Rui F. T2 - Functional Ecology A2 - Grindstaff, Jennifer DA - 2015/12// PY - 2015 DO - 10.1111/1365-2435.12465 VL - 29 IS - 12 SP - 1533 EP - 1542 J2 - Funct Ecol LA - en SN - 0269-8463, 1365-2435 UR - https://onlinelibrary.wiley.com/doi/abs/10.1111/1365-2435.12465 Y2 - 2021/02/19/08:51:47 ER - TY - JOUR TI - Social cues in the expression of sequential alternative reproductive tactics in young males of the peacock blenny, Salaria pavo AU - Fagundes, Teresa AU - Simões, Mariana G. AU - Gonçalves, David AU - Oliveira, Rui F. T2 - Physiology & Behavior DA - 2012/10// PY - 2012 DO - 10.1016/j.physbeh.2012.07.011 VL - 107 IS - 3 SP - 283 EP - 291 J2 - Physiology & Behavior LA - en SN - 00319384 UR - https://linkinghub.elsevier.com/retrieve/pii/S0031938412002740 Y2 - 2021/02/19/09:01:16 ER - TY - JOUR TI - Efficient isolation of polymorphic microsatellites from high-throughput sequence data based on number of repeats AU - Cardoso, Sara D. AU - Gonçalves, David AU - Robalo, Joana I. AU - Almada, Vitor C. AU - Canário, Adelino V.M. AU - Oliveira, Rui F. T2 - Marine Genomics DA - 2013/09// PY - 2013 DO - 10.1016/j.margen.2013.04.002 VL - 11 SP - 11 EP - 16 J2 - Marine Genomics LA - en SN - 18747787 UR - https://linkinghub.elsevier.com/retrieve/pii/S1874778713000226 Y2 - 2021/02/19/08:56:47 ER - TY - JOUR TI - Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish AU - Cardoso, Sara D. AU - Gonçalves, David AU - Goesmann, Alexander AU - Canário, Adelino V. M. AU - Oliveira, Rui F. T2 - Molecular Ecology AB - Distinct patterns of gene expression often underlie intra- and intersexual differences, and the study of this set of coregulated genes is essential to understand the emergence of complex behavioural phenotypes. Here, we describe the development of a de novo transcriptome and brain gene expression profiles of wild-caught peacock blenny, Salaria pavo, an intertidal fish with sex-role reversal in courtship behaviour (i.e., females are the courting sex) and sequential alternative reproductive tactics in males (i.e., larger and older nest-holder males and smaller and younger sneaker males occur). Sneakers mimic both female's courtship behaviour and nuptial coloration to get access to nests and sneak fertilizations, and later in life transition into nest-holder males. Thus, this species offers the unique opportunity to study how the regulation of gene expression can contribute to intersex phenotypes and to the sequential expression of male and female behavioural phenotypes by the same individual. We found that at the whole brain level, expression of the sneaker tactic was paralleled by broader and divergent gene expression when compared to either females or nest-holder males, which were more similar between themselves. When looking at sex-biased transcripts, sneaker males are intersex rather than being either nest-holder or female-like, and their transcriptome is simultaneously demasculinized for nest-holder-biased transcripts and feminized for female-biased transcripts. These results indicate that evolutionary changes in reproductive plasticity can be achieved through regulation of gene expression, and in particular by varying the magnitude of expression of sex-biased genes, throughout the lifetime of the same individual. DA - 2018/// PY - 2018 DO - https://doi.org/10.1111/mec.14408 VL - 27 IS - 3 SP - 789 EP - 803 LA - en SN - 1365-294X UR - https://onlinelibrary.wiley.com/doi/abs/10.1111/mec.14408 Y2 - 2021/02/10/10:37:37 KW - RNA-seq KW - Salaria pavo KW - alternative reproductive tactics KW - behavioural plasticity KW - neurogenomic state KW - sex-biased gene expression ER - TY - JOUR TI - Social network predicts loss of fertilizations in nesting males of a fish with alternative reproductive tactics AU - Cardoso, Sara D. AU - Faustino, Ana I. AU - Costa, Silvia S. AU - Valério, Fábio AU - Gonçalves, David AU - Oliveira, Rui F. T2 - acta ethologica AB - Alternative reproductive tactics (ARTs) evolve when there is strong intra-sexual competition between conspecifics for access to mates. Typically, larger “bourgeois” males reproduce by securing the access to reproductive resources while smaller “parasitic” males reproduce by stealing fertilizations from larger males. A number of factors can influence the reproductive success of each tactic, including intrinsic (e.g. size) and extrinsic (e.g. tactic relative frequency) variables. An example where plastic ARTs occur is the peacock blenny Salaria pavo, with large males reproducing by defending nests and attracting females (bourgeois tactic) and small males reproducing by achieving sneaked fertilizations (parasitic tactic). In this study, we conducted field observations on individually tagged animals to determine their social network and collected eggs from 11 nests to determine the fertilization success of each male tactic. Paternity estimates for 550 offspring indicated an average fertilization success for nest-holder males of 95%. Nest-holder male morphological traits and social network parameters were tested as predictors of fertilization success, but only the number of sneakers present in the nest-holder’s social networks was found to be a predictor of paternity loss. Although male morphological traits had been previously found to be strongly correlated with reproductive success of nest-holder males, as measured by the number of eggs collected in the male’s nest, no correlation was found between any of the measured morphological traits and fertilization success for these males. The results suggest a stronger influence of the social environment than of morphological variables in the proportion of lost fertilizations by nest-holder males of this species. DA - 2017/02/01/ PY - 2017 DO - 10.1007/s10211-016-0249-9 VL - 20 IS - 1 SP - 59 EP - 68 J2 - acta ethol LA - en SN - 1437-9546 UR - https://doi.org/10.1007/s10211-016-0249-9 Y2 - 2021/02/01/05:08:39 ER - TY - JOUR TI - Personal Care and Cosmetic Products as a Potential Source of Environmental Contamination by Microplastics in a Densely Populated Asian City AU - Bashir, Saidu M. AU - Kimiko, Sam AU - Mak, Chu-Wa AU - Fang, James Kar-Hei AU - Gonçalves, David T2 - Frontiers in Marine Science AB - The prevalence of microplastics in the environment has become a major global conservation issue. One primary source of environmental microplastics is personal care and cosmetic products (PCCPs) containing microbeads. The market availability of PCCPs containing microbeads and the level of contamination of coastal sediments by microplastics was studied in one of the most densely populated cities in the world, Macao in China. We found that PCCPs containing microbeads are still widely available for sale in the region, with over 70% of surveyed PCCPs containing at least one type of microbeads as an ingredient, with polyethylene (PE) being the most common one. In an estimate, the use of PCCPs in the territory may release over 37 billion microbeads per year into the environment via wastewater treatment plants. The density of microplastics in coastal sediments varied between 259 and 1,743 items/L of sediment, amongst the highest reported in the world. The fraction of < 1 mm was the most abundant, representing an average of 98.6% of the total, and correlated positively with the abundance of larger sized fragments. The results show that although environmental pollution with microplastics released from PCCPs usage is significant, other sources, namely fragmentation of larger plastic debris, likely contribute more to the issue. The study highlights the magnitude of the problem at a local level and suggests possible mitigating strategies. DA - 2021/// PY - 2021 DP - Frontiers VL - 8 SN - 2296-7745 UR - https://www.frontiersin.org/articles/10.3389/fmars.2021.683482 Y2 - 2023/04/11/13:47:08 ER - TY - JOUR TI - Behavioural and endocrine responses to non-interactive live and video conspecifics in males of the Siamese fighting fish AU - Alex, Deepa AU - Cardoso, Sara D AU - Ramos, Andreia AU - Gonçalves, David T2 - Current Zoology AB - The physiological mechanisms underlying variation in aggression in fish remain poorly understood. One possibly confounding variable is the lack of standardization in the type of stimuli used to elicit aggression. The presentation of controlled stimuli in videos, a.k.a. video playback, can provide better control of the fight components. However, this technique has produced conflicting results in animal behaviour studies and needs to be carefully validated. For this, a similar response to the video and an equivalent live stimulus needs to be demonstrated. Further, different physiological responses may be triggered by live and video stimuli and it is important to demonstrate that video images elicit appropriate physiological reactions. Here, the behavioural and endocrine response of male Siamese fighting fish Betta splendens to a matched for size conspecific fighting behind a one-way mirror, presented live or through video playback, was compared. The video playback and live stimulus elicited a strong and similar aggressive response by the focal fish, with a fight structure that started with stereotypical threat displays and progressed to overt attacks. Post-fight plasma levels of the androgen 11-ketotestosterone were elevated as compared to controls, regardless of the type of stimuli. Cortisol also increased in response to the video images, as previously described for live fights in this species. These results show that the interactive component of a fight, and its resolution, are not needed to trigger an endocrine response to aggression in this species. The study also demonstrates for the first time in a fish a robust endocrine response to video stimuli and supports the use of this technique for researching aggressive behaviour in B. splendens. DA - 2022/10/08/ PY - 2022 DO - 10.1093/cz/zoac078 DP - Silverchair SP - zoac078 J2 - Current Zoology SN - 2396-9814 UR - https://doi.org/10.1093/cz/zoac078 Y2 - 2022/11/09/06:01:40 ER -