@article{zhang_effects_2023, title = {Effects of a {High}-{Fat} {Diet} on {Intestinal} and {Gonadal} {Metabolism} in {Female} and {Male} {Sea} {Cucumber} {Apostichopus} japonicus}, volume = {12}, copyright = {http://creativecommons.org/licenses/by/3.0/}, issn = {2079-7737}, url = {https://www.mdpi.com/2079-7737/12/2/212}, doi = {10.3390/biology12020212}, abstract = {Parental nutrient reserves are directly related to reproductive performance in sea cucumbers. This study focused on the lipid requirements of male and female sea cucumbers Apostichopus japonicus during the reproductive stage and analyzed their physiological responses to a high-fat diet (HFD). The intestinal lipid metabolites and microbiome profile changed significantly in animals fed with the HFD, as given by an upregulation of metabolites related to lipid metabolism and an increase in the predominance of Proteobacteria in the microbiome, respectively. The metabolic responses of male and female sea cucumbers to the HFD differed, which in turn could have triggered sex-related differences in the intestinal microbiome. These results suggest that the lipid content in diets can be differentially adjusted for male and female sea cucumbers to improve nutrition and promote reproduction. This data contributes to a better understanding of the reproductive biology and sex differences of sea cucumbers.}, language = {en}, number = {2}, urldate = {2023-04-11}, journal = {Biology}, author = {Zhang, Shuangyan and Ru, Xiaoshang and Zhang, Libin and Gonçalves, David and Yang, Hongsheng and Xu, Jialei}, month = feb, year = {2023}, note = {Number: 2 Publisher: Multidisciplinary Digital Publishing Institute}, keywords = {high-fat diet, intestinal microbes, physiological metabolic responses, sea cucumber, sex differences}, pages = {212}, } @article{zhang_examination_2023, title = {Examination of sex-related differences in intestinal and gonadal lipid metabolism in the sea cucumber {Apostichopus} japonicus}, volume = {562}, issn = {0044-8486}, url = {https://www.sciencedirect.com/science/article/pii/S0044848622009048}, doi = {10.1016/j.aquaculture.2022.738787}, abstract = {Reproduction of the sea cucumber Apostichopus japonicus is critical for aquaculture production. Gonadal development is the basis of reproduction, and lipids, which are among the main nutrients required for gonadal development, directly affect reproduction. We investigated whether gonadal and intestinal lipid metabolism differed between male and female A. japonicus. Transcriptome analysis of the intestines of sexually mature male and female wild-caught individuals revealed differences in gene expression, with 27 and 39 genes being up-regulated in females and males, respectively. In particular, the expression of the fatty acid synthase gene was higher in males than in females. Metabolome analysis of the gonads identified 141 metabolites that were up-regulated and 175 metabolites that were down-regulated in the testes compared with the ovaries in the positive/negative mode of an LC-MS/MS analysis. A variety of polyunsaturated fatty acids were found at higher concentrations in the testes than in the ovaries. 16 s rDNA sequencing analysis showed that the composition and structure of the intestinal microbiota were similar between males and females. These results suggest that sex differences in intestinal metabolism of A. japonicus are not due to differences in the microbiota, and we speculate that gonadal metabolism may be related to intestinal morphology. This information might be useful in improving the reproductive efficiency of sea cucumbers in captivity.}, language = {en}, urldate = {2023-04-11}, journal = {Aquaculture}, author = {Zhang, Shuangyan and Ru, Xiaoshang and Zhang, Libin and Gonçalves, David and Yang, Hongsheng}, month = jan, year = {2023}, keywords = {Gonads, Intestines, Lipid metabolism, Sea cucumber, Sex differences}, pages = {738787}, } @article{alex_behavioural_2022, title = {Behavioural and endocrine responses to non-interactive live and video conspecifics in males of the {Siamese} fighting fish}, issn = {2396-9814}, url = {https://doi.org/10.1093/cz/zoac078}, doi = {10.1093/cz/zoac078}, abstract = {The physiological mechanisms underlying variation in aggression in fish remain poorly understood. One possibly confounding variable is the lack of standardization in the type of stimuli used to elicit aggression. The presentation of controlled stimuli in videos, a.k.a. video playback, can provide better control of the fight components. However, this technique has produced conflicting results in animal behaviour studies and needs to be carefully validated. For this, a similar response to the video and an equivalent live stimulus needs to be demonstrated. Further, different physiological responses may be triggered by live and video stimuli and it is important to demonstrate that video images elicit appropriate physiological reactions. Here, the behavioural and endocrine response of male Siamese fighting fish Betta splendens to a matched for size conspecific fighting behind a one-way mirror, presented live or through video playback, was compared. The video playback and live stimulus elicited a strong and similar aggressive response by the focal fish, with a fight structure that started with stereotypical threat displays and progressed to overt attacks. Post-fight plasma levels of the androgen 11-ketotestosterone were elevated as compared to controls, regardless of the type of stimuli. Cortisol also increased in response to the video images, as previously described for live fights in this species. These results show that the interactive component of a fight, and its resolution, are not needed to trigger an endocrine response to aggression in this species. The study also demonstrates for the first time in a fish a robust endocrine response to video stimuli and supports the use of this technique for researching aggressive behaviour in B. splendens.}, urldate = {2022-11-09}, journal = {Current Zoology}, author = {Alex, Deepa and Cardoso, Sara D and Ramos, Andreia and Gonçalves, David}, month = oct, year = {2022}, pages = {zoac078}, } @article{ramos_selection_2022, title = {Selection for winners impacts the endocrine system in the {Siamese} fighting fish}, volume = {318}, issn = {00166480}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0016648022000132}, doi = {10.1016/j.ygcen.2022.113988}, abstract = {In southeast Asia, males of the Siamese fighting fish, Betta splendens, have been selected across centuries for winning paired staged fights and previous work has shown that males from fighter strains are more aggressive than wild-types. This strong directional selection for winners is likely to have targeted aggression-related endocrine systems, and a comparison between fighter and wild-type strains can bring into evidence the key hormones implicated in aggression. Here, we compared the plasma levels of the androgen 11-ketotestosterone (KT) and of the corticosteroid cortisol (F) in F2 males of a fighter and a wild-type strain raised under similar laboratory conditions. We show that F was generally lower in fighter as compared with wild-type males, while no overall differences in KT levels were detected between strains. When presented with a mirror-induced aggressive challenge, post-fight levels of F increased but more significantly so in wild-type males, while KT increased in males of both strains. After the challenge, fighter males had higher levels of KT as compared with wild-type males, while the pattern for F was opposite. As compared with animals in social groups, wild-type males placed under social isolation had lower F levels, while KT decreased for fighters. Taken together, this data suggests that while wild-type males responded to aggression with an increase in circulating levels of both androgens and corticosteroids, males selected for winning fights maintained a blunt F response, increasing only KT levels. These data agree with the hypothesis that a combination of high levels of androgens and low levels of corticosteroids is associated with high aggression. Overall, these results seem to indicate that selection for winning had a stronger impact in the hypothalamus-pituitary-interrenal axis than in the hypothalamus-pituitary–gonadal axis in B. splendens.}, language = {en}, urldate = {2023-04-11}, journal = {General and Comparative Endocrinology}, author = {Ramos, A. and Gonçalves, D.}, month = mar, year = {2022}, pages = {113988}, } @article{moreira_sediments_2021, title = {Sediments in the mangrove areas contribute to the removal of endocrine disrupting chemicals in coastal sediments of {Macau} {SAR}, {China}, and harbour microbial communities capable of degrading {E2}, {EE2}, {BPA} and {BPS}}, volume = {32}, issn = {1572-9729}, url = {https://doi.org/10.1007/s10532-021-09948-9}, doi = {10.1007/s10532-021-09948-9}, abstract = {The occurrence of endocrine disrupting chemicals (EDCs) is a major issue for marine and coastal environments in the proximity of urban areas. The occurrence of EDCs in the Pearl River Delta region is well documented but specific data related to Macao is unavailable. The levels of bisphenol-A (BPA), estrone (E1), 17α-estradiol (αE2), 17β-estradiol (E2), estriol (E3), and 17α-ethynylestradiol (EE2) were measured in sediment samples collected along the coastline of Macao. BPA was found in all 45 collected samples with lower BPA concentrations associated to the presence of mangrove trees. Biodegradation assays were performed to evaluate the capacity of the microbial communities of the surveyed ecosystems to degrade BPA and its analogue BPS. Using sediments collected at a WWTP discharge point as inoculum, at a concentration of 2 mg l−1 complete removal of BPA was observed within 6 days, whereas for the same concentration BPS removal was of 95\% after 10 days, which is particularly interesting since this compound is considered recalcitrant to biodegradation and likely to accumulate in the environment. Supplementation with BPA improved the degradation of bisphenol-S (BPS). Aiming at the isolation of EDCs-degrading bacteria, enrichments were established with sediments supplied with BPA, BPS, E2 and EE2, which led to the isolation of a bacterial strain, identified as Rhodoccoccus sp. ED55, able to degrade the four compounds at different extents. The isolated strain represents a valuable candidate for bioremediation of contaminated soils and waters.}, language = {en}, number = {5}, urldate = {2023-04-11}, journal = {Biodegradation}, author = {Moreira, Irina S. and Lebel, Alexandre and Peng, Xianzhi and Castro, Paula M. L. and Gonçalves, David}, month = oct, year = {2021}, keywords = {Biodegradation, Bisphenols, EDCs-degrading bacteria, Endocrine disrupting chemicals (EDCs), Estrogens, Mangrove}, pages = {511--529}, } @article{ramos_androgens_2021, title = {Androgens and corticosteroids increase in response to mirror images and interacting conspecifics in males of the {Siamese} fighting fish {Betta} splendens}, volume = {132}, issn = {0018-506X}, url = {https://www.sciencedirect.com/science/article/pii/S0018506X21000702}, doi = {10.1016/j.yhbeh.2021.104991}, abstract = {The role of hormones as modulators of aggressive behavior in fish remains poorly understood. Androgens and corticosteroids, in particular, have been associated with aggressive behavior in fish but it is still not clear if animals adjust the secretion of these hormones to regulate behavior during ongoing fights, in response to fight outcomes in order to adjust aggressive behavior in subsequent fights, or both. With its stereotyped displays and high aggression levels, the Siamese fighting fish Betta splendens is an excellent model to investigate this question. Here, we compared the behavioral and endocrine response of male B. splendens to fights where there is no winner or loser by presenting them with a size-matched live interacting conspecific behind a transparent partition or with a mirror image. The aggressive response started with threat displays that were overall similar in frequency and duration towards both types of stimuli. Fights transitioned to overt attacks and interacting with a live conspecific elicited a higher frequency of attempted bites and head hits, as compared with the mirror image. There was a pronounced increase in plasma androgens (11-ketotestosterone and testosterone) and corticosteroids (cortisol) levels in response to the aggression challenge, independent of stimulus type. Post-fight intra-group levels of these hormones did not correlate with measures of physical activity or aggressive behavior. A linear discriminant analysis including all behavioral and endocrine data was a poor classifier of fish from the conspecific and mirror trials, showing that overall the behavioral and endocrine response to mirror images and conspecifics was similar. The results show that fight resolution is not necessary to induce an evident increase in peripheral levels of androgens and corticosteroids in B. splendens. However, the function of these hormones during present and future aggressive contests remains to be clarified.}, language = {en}, urldate = {2023-04-11}, journal = {Hormones and Behavior}, author = {Ramos, Andreia and Alex, Deepa and Cardoso, Sara D. and Gonçalves, David}, month = jun, year = {2021}, keywords = {Aggression, Androgens, Corticosteroids, Live conspecific test, Mirror test}, pages = {104991}, } @article{bashir_personal_2021, title = {Personal {Care} and {Cosmetic} {Products} as a {Potential} {Source} of {Environmental} {Contamination} by {Microplastics} in a {Densely} {Populated} {Asian} {City}}, volume = {8}, issn = {2296-7745}, url = {https://www.frontiersin.org/articles/10.3389/fmars.2021.683482}, abstract = {The prevalence of microplastics in the environment has become a major global conservation issue. One primary source of environmental microplastics is personal care and cosmetic products (PCCPs) containing microbeads. The market availability of PCCPs containing microbeads and the level of contamination of coastal sediments by microplastics was studied in one of the most densely populated cities in the world, Macao in China. We found that PCCPs containing microbeads are still widely available for sale in the region, with over 70\% of surveyed PCCPs containing at least one type of microbeads as an ingredient, with polyethylene (PE) being the most common one. In an estimate, the use of PCCPs in the territory may release over 37 billion microbeads per year into the environment via wastewater treatment plants. The density of microplastics in coastal sediments varied between 259 and 1,743 items/L of sediment, amongst the highest reported in the world. The fraction of {\textless} 1 mm was the most abundant, representing an average of 98.6\% of the total, and correlated positively with the abundance of larger sized fragments. The results show that although environmental pollution with microplastics released from PCCPs usage is significant, other sources, namely fragmentation of larger plastic debris, likely contribute more to the issue. The study highlights the magnitude of the problem at a local level and suggests possible mitigating strategies.}, urldate = {2023-04-11}, journal = {Frontiers in Marine Science}, author = {Bashir, Saidu M. and Kimiko, Sam and Mak, Chu-Wa and Fang, James Kar-Hei and Gonçalves, David}, year = {2021}, } @inproceedings{lei_statistical_2021, address = {Berlin, Heidelberg}, series = {Springer {Proceedings} in {Complexity}}, title = {Statistical {Methods} to {Forecast} {Air} {Quality} in {Taipa} {Ambient} and {Taipa} {Residential} of {Macao}}, isbn = {978-3-662-63760-9}, doi = {10.1007/978-3-662-63760-9_25}, abstract = {Air pollution is a major concern issue on Macao since the concentration levels of several of the most common pollutants are frequently above the internationally recommended values. The low air quality episodes impacts on human health paired with highly populated urban areas are important motivations to develop forecast methodologies in order to anticipate pollution episodes, allowing establishing warnings to the local community to take precautionary measures and avoid outdoor activities during this period. Using statistical methods (multiple linear regression (MLR) and classification and regression tree (CART) analysis) we were able to develop forecasting models for the main pollutants (NO2, PM2.5, and O3) enabling us to know the next day concentrations with a good skill, translated by high coefficients of determination (0.82–0.90) on a 95\% confidence level. The model development was based on six years of historical data, 2013 to 2018, consisting of surface and upper-air meteorological observations and surface air quality observations. The year of 2019 was used for model validation. From an initially large group of meteorological and air quality variables only a few were identified as significant dependent variables in the model. The selected meteorological variables included geopotential height, relative humidity and air temperature at different altitude levels and atmospheric stability characterization parameters. The air quality predictors used included recent past hourly levels of mean concentrations for NO2 and PM2.5 and maximum concentrations for O3. The application of the obtained models provides the expected daily mean concentrations for NO2 and PM2.5 and maximum hourly concentrations O3 for the next day in Taipa Ambient air quality monitoring stations. The described methodology is now operational, in Macao, since 2020.}, language = {en}, booktitle = {Air {Pollution} {Modeling} and its {Application} {XXVII}}, publisher = {Springer}, author = {Lei, Man Tat and Monjardino, Joana and Mendes, Luisa and Gonçalves, David and Ferreira, Francisco}, editor = {Mensink, Clemens and Matthias, Volker}, year = {2021}, note = {0 citations (Crossref) [2022-09-21]}, pages = {167--173}, } @article{silva_physiology_2021, title = {Physiology, endocrinology and chemical communication in aggressive behaviour of fishes}, issn = {0022-1112, 1095-8649}, url = {https://onlinelibrary.wiley.com/doi/10.1111/jfb.14667}, doi = {10.1111/jfb.14667}, abstract = {Fishes show remarkably diverse aggressive behaviour. Aggression is expressed to secure resources; adjusting aggression levels according to context is key to avoid negative consequences for fitness and survival. Nonetheless, despite its importance, the physiological basis of aggression in fishes is still poorly understood. Several reports suggest hormonal modulation of aggression, particularly by androgens, but contradictory studies have been published. Studies exploring the role of chemical communication in aggressive behaviour are also scant, and the pheromones involved remain to be unequivocally characterized. This is surprising as chemical communication is the most ancient form of information exchange and plays a variety of other roles in fishes. Furthermore, the study of chemical communication and aggression is relevant at the evolutionary, ecological and economic levels. A few pioneering studies support the hypothesis that aggressive behaviour, at least in some teleosts, is modulated by “dominance pheromones” that reflect the social status of the sender, but there is little information on the identity of the compounds involved. This review aims to provide a global view of aggressive behaviour in fishes and its underlying physiological mechanisms including the involvement of chemical communication, and discusses the potential use of dominance pheromones to improve fish welfare. Methodological considerations and future research directions are also outlined.}, language = {en}, urldate = {2021-01-26}, journal = {Journal of Fish Biology}, author = {Silva, Melina Coelho da and Canário, Adelino Vicente Mendonça and Hubbard, Peter Colin and Gonçalves, David}, year = {2021}, note = {3 citations (Crossref) [2022-09-21]}, } @article{lei_statistical_2020, title = {Statistical {Forecast} {Applied} to {Two} {Macao} {Air} {Monitoring} {Stations}}, volume = {489}, issn = {1755-1315}, url = {https://doi.org/10.1088/1755-1315/489/1/012018}, doi = {10.1088/1755-1315/489/1/012018}, abstract = {The levels of air pollution in Macao often exceeded the levels recommended by WHO. In order for the population to take precautionary measures and avoid further health risks under high pollutant exposure, it is important to develop a reliable air quality forecast. Statistical models based on multiple regression (MR) analysis were developed successfully for Macao to predict the next day concentrations of PM10, PM2.5, and NO2. All the developed models were statistically significantly valid with a 95\% confidence level with high coefficients of determination (from 0.89 to 0.92) for all pollutants. The models utilized meteorological and air quality variables based on five years of historical data, from 2013 to 2017. The data from 2013 to 2016 were used to develop the statistical models and data from 2017 were used for validation purposes. A wide range of meteorological and air quality variables were identified, and only some were selected as significant dependent variables. Meteorological variables were selected from an extensive list of variables, including geopotential height, relative humidity, atmospheric stability, and air temperature at different vertical levels. Air quality variables translate the resilience of the recent past concentrations of each pollutant and usually are maximum and/or the average of latest 24-hour levels. The models were applied in forecasting the next day average daily concentrations for PM10, PM2.5, and NO2 for the air quality monitoring stations. The results are expected to be an operational air quality forecast for Macao.}, language = {en}, number = {1}, urldate = {2022-09-21}, journal = {IOP Conference Series: Earth and Environmental Science}, author = {Lei, M. and Monjardino, J. and Mendes, L. and Gonçalves, D. and Ferreira, F.}, month = apr, year = {2020}, note = {0 citations (Crossref) [2022-09-21] Publisher: IOP Publishing}, pages = {012018}, } @article{lei_statistical_2020, title = {Statistical {Forecast} of {Pollution} {Episodes} in {Macao} during {National} {Holiday} and {COVID}-19}, volume = {17}, copyright = {http://creativecommons.org/licenses/by/3.0/}, issn = {1660-4601}, url = {https://www.mdpi.com/1660-4601/17/14/5124}, doi = {10.3390/ijerph17145124}, abstract = {Statistical methods such as multiple linear regression (MLR) and classification and regression tree (CART) analysis were used to build prediction models for the levels of pollutant concentrations in Macao using meteorological and air quality historical data to three periods: (i) from 2013 to 2016, (ii) from 2015 to 2018, and (iii) from 2013 to 2018. The variables retained by the models were identical for nitrogen dioxide (NO2), particulate matter (PM10), PM2.5, but not for ozone (O3) Air pollution data from 2019 was used for validation purposes. The model for the 2013 to 2018 period was the one that performed best in prediction of the next-day concentrations levels in 2019, with high coefficient of determination (R2), between predicted and observed daily average concentrations (between 0.78 and 0.89 for all pollutants), and low root mean square error (RMSE), mean absolute error (MAE), and biases (BIAS). To understand if the prediction model was robust to extreme variations in pollutants concentration, a test was performed under the circumstances of a high pollution episode for PM2.5 and O3 during 2019, and the low pollution episode during the period of implementation of the preventive measures for COVID-19 pandemic. Regarding the high pollution episode, the period of the Chinese National Holiday of 2019 was selected, in which high concentration levels were identified for PM2.5 and O3, with peaks of daily concentration exceeding 55 μg/m3 and 400 μg/m3, respectively. The 2013 to 2018 model successfully predicted this high pollution episode with high coefficients of determination (of 0.92 for PM2.5 and 0.82 for O3). The low pollution episode for PM2.5 and O3 was identified during the 2020 COVID-19 pandemic period, with a low record of daily concentration for PM2.5 levels at 2 μg/m3 and O3 levels at 50 μg/m3, respectively. The 2013 to 2018 model successfully predicted the low pollution episode for PM2.5 and O3 with a high coefficient of determination (0.86 and 0.84, respectively). Overall, the results demonstrate that the statistical forecast model is robust and able to correctly reproduce extreme air pollution events of both high and low concentration levels.}, language = {en}, number = {14}, urldate = {2022-09-21}, journal = {International Journal of Environmental Research and Public Health}, author = {Lei, Man Tat and Monjardino, Joana and Mendes, Luisa and Gonçalves, David and Ferreira, Francisco}, month = jan, year = {2020}, note = {8 citations (Crossref) [2022-09-21] Number: 14 Publisher: Multidisciplinary Digital Publishing Institute}, keywords = {COVID-19, air pollution, air quality forecast, modelling, national holiday, pollution episodes}, pages = {5124}, } @article{lei_macao_2019, title = {Macao air quality forecast using statistical methods}, volume = {12}, issn = {1873-9326}, url = {https://doi.org/10.1007/s11869-019-00721-9}, doi = {10.1007/s11869-019-00721-9}, abstract = {The levels of air pollution in Macao often exceeded the levels recommended by WHO. In order for the population to take precautionary measures and avoid further health risks under high pollutant exposure, it is important to develop a reliable air quality forecast. Statistical models based on linear multiple regression (MR) and classification and regression trees (CART) analysis were developed successfully, for Macao, to predict the next day concentrations of NO2, PM10, PM2.5, and O3. All the developed models were statistically significantly valid with a 95\% confidence level with high coefficients of determination (from 0.78 to 0.93) for all pollutants. The models utilized meteorological and air quality variables based on 5 years of historical data, from 2013 to 2017. Data from 2013 to 2016 were used to develop the statistical models and data from 2017 was used for validation purposes. A wide range of meteorological and air quality variables was identified, and only some were selected as significant independent variables. Meteorological variables were selected from an extensive list of variables, including geopotential height, relative humidity, atmospheric stability, and air temperature at different vertical levels. Air quality variables translate the resilience of the recent past concentrations of each pollutant and usually are maximum and/or the average of latest 24-h levels. The models were applied in forecasting the next day average daily concentrations for NO2 and PM and maximum hourly O3 levels for five air quality monitoring stations. The results are expected to be an operational air quality forecast for Macao.}, language = {en}, number = {9}, urldate = {2022-09-21}, journal = {Air Quality, Atmosphere \& Health}, author = {Lei, Man Tat and Monjardino, Joana and Mendes, Luisa and Gonçalves, David and Ferreira, Francisco}, month = sep, year = {2019}, note = {24 citations (Crossref) [2022-09-21]}, keywords = {Macao, NO2, O3, PM10, PM2.5, Particulate matter}, pages = {1049--1057}, } @article{ramos_artificial_2019, title = {Artificial selection for male winners in the {Siamese} fighting fish {Betta} splendens correlates with high female aggression}, volume = {16}, issn = {1742-9994}, url = {https://doi.org/10.1186/s12983-019-0333-x}, doi = {10.1186/s12983-019-0333-x}, abstract = {In Southeast Asia, males of the Siamese fighting fish Betta splendens have been selected across centuries for paired-staged fights. During the selection process, matched for size males fight in a small tank until the contest is resolved. Breeders discard losing batches and reproduce winner batches with the aim of increasing fight performance. We assessed the results of this long-term selection process by comparing under standard laboratory conditions male and female aggressive behaviour of one strain selected for staged fights (“fighters”) and one strain of wild-types. The aggressive response of adult fish was tested against their mirror image or a size-matched conspecific. Fighter males were more aggressive than wild-type males for all measured behaviours. Differences were not only quantitative but the pattern of fight display was also divergent. Fighter males had an overall higher swimming activity, performing frequent fast strikes in the direction of the intruder and displaying from a distance. Wild-type males were less active and exhibited aggressive displays mostly in close proximity to the stimuli. Females of the fighter strain, which are not used for fights, were also more aggressive than wild-type females. Aggressive behaviours were correlated across male and female fighter siblings, suggesting common genetic and physiological mechanisms to male and female aggression in this species. The study further shows that results were largely independent of the stimulus type, with the mirror test inducing similar and less variable responses than the live conspecific presentation. These results suggest that selection for male winners co-selected for high-frequency and metabolic demanding aggressive display in males and also enhanced female aggression, opening a wide range of testable hypothesis about the ultimate and proximate mechanisms of male and female aggression in B. splendens.}, number = {1}, urldate = {2023-04-11}, journal = {Frontiers in Zoology}, author = {Ramos, A. and Gonçalves, D.}, month = aug, year = {2019}, keywords = {Aggression, Artificial selection, Domestication, Mirror test, Sexual conflict}, pages = {34}, } @article{fan_chromosome-level_2018, title = {Chromosome-level reference genome of the {Siamese} fighting fish {Betta} splendens, a model species for the study of aggression}, issn = {2047-217X}, url = {https://academic.oup.com/gigascience/advance-article/doi/10.1093/gigascience/giy087/5054042}, doi = {10.1093/gigascience/giy087}, language = {en}, urldate = {2021-02-19}, journal = {GigaScience}, author = {Fan, Guangyi and Chan, Judy and Ma, Kailong and Yang, Binrui and Zhang, He and Yang, Xianwei and Shi, Chengcheng and Law, Henry and Ren, Zhitao and Xu, Qiwu and Liu, Qun and Wang, Jiahao and Chen, Wenbin and Shao, Libin and Gonçalves, David and Ramos, Andreia and Cardoso, Sara D and Guo, Min and Cai, Jing and Xu, Xun and Wang, Jian and Yang, Huanming and Liu, Xin and Wang, Yitao}, month = jul, year = {2018}, note = {9 citations (Crossref) [2022-09-21]}, } @article{cardoso_temporal_2018, title = {Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish}, volume = {27}, copyright = {© 2017 John Wiley \& Sons Ltd}, issn = {1365-294X}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/mec.14408}, doi = {https://doi.org/10.1111/mec.14408}, abstract = {Distinct patterns of gene expression often underlie intra- and intersexual differences, and the study of this set of coregulated genes is essential to understand the emergence of complex behavioural phenotypes. Here, we describe the development of a de novo transcriptome and brain gene expression profiles of wild-caught peacock blenny, Salaria pavo, an intertidal fish with sex-role reversal in courtship behaviour (i.e., females are the courting sex) and sequential alternative reproductive tactics in males (i.e., larger and older nest-holder males and smaller and younger sneaker males occur). Sneakers mimic both female's courtship behaviour and nuptial coloration to get access to nests and sneak fertilizations, and later in life transition into nest-holder males. Thus, this species offers the unique opportunity to study how the regulation of gene expression can contribute to intersex phenotypes and to the sequential expression of male and female behavioural phenotypes by the same individual. We found that at the whole brain level, expression of the sneaker tactic was paralleled by broader and divergent gene expression when compared to either females or nest-holder males, which were more similar between themselves. When looking at sex-biased transcripts, sneaker males are intersex rather than being either nest-holder or female-like, and their transcriptome is simultaneously demasculinized for nest-holder-biased transcripts and feminized for female-biased transcripts. These results indicate that evolutionary changes in reproductive plasticity can be achieved through regulation of gene expression, and in particular by varying the magnitude of expression of sex-biased genes, throughout the lifetime of the same individual.}, language = {en}, number = {3}, urldate = {2021-02-10}, journal = {Molecular Ecology}, author = {Cardoso, Sara D. and Gonçalves, David and Goesmann, Alexander and Canário, Adelino V. M. and Oliveira, Rui F.}, year = {2018}, note = {6 citations (Crossref) [2022-09-21] \_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.14408}, keywords = {RNA-seq, Salaria pavo, alternative reproductive tactics, behavioural plasticity, neurogenomic state, sex-biased gene expression}, pages = {789--803}, } @techreport{goncalves_fight_2018, address = {Macao}, type = {Research {Report}}, title = {{FIGHT} – {Hormonal}, pheromonal and genomic regulation of aggression in a fish model}, institution = {University of Saint Joseph}, author = {Gonçalves, David}, year = {2018}, } @article{cardoso_social_2017, title = {Social network predicts loss of fertilizations in nesting males of a fish with alternative reproductive tactics}, volume = {20}, issn = {1437-9546}, url = {https://doi.org/10.1007/s10211-016-0249-9}, doi = {10.1007/s10211-016-0249-9}, abstract = {Alternative reproductive tactics (ARTs) evolve when there is strong intra-sexual competition between conspecifics for access to mates. Typically, larger “bourgeois” males reproduce by securing the access to reproductive resources while smaller “parasitic” males reproduce by stealing fertilizations from larger males. A number of factors can influence the reproductive success of each tactic, including intrinsic (e.g. size) and extrinsic (e.g. tactic relative frequency) variables. An example where plastic ARTs occur is the peacock blenny Salaria pavo, with large males reproducing by defending nests and attracting females (bourgeois tactic) and small males reproducing by achieving sneaked fertilizations (parasitic tactic). In this study, we conducted field observations on individually tagged animals to determine their social network and collected eggs from 11 nests to determine the fertilization success of each male tactic. Paternity estimates for 550 offspring indicated an average fertilization success for nest-holder males of 95\%. Nest-holder male morphological traits and social network parameters were tested as predictors of fertilization success, but only the number of sneakers present in the nest-holder’s social networks was found to be a predictor of paternity loss. Although male morphological traits had been previously found to be strongly correlated with reproductive success of nest-holder males, as measured by the number of eggs collected in the male’s nest, no correlation was found between any of the measured morphological traits and fertilization success for these males. The results suggest a stronger influence of the social environment than of morphological variables in the proportion of lost fertilizations by nest-holder males of this species.}, language = {en}, number = {1}, urldate = {2021-02-01}, journal = {acta ethologica}, author = {Cardoso, Sara D. and Faustino, Ana I. and Costa, Silvia S. and Valério, Fábio and Gonçalves, David and Oliveira, Rui F.}, month = feb, year = {2017}, note = {3 citations (Crossref) [2022-09-21]}, pages = {59--68}, } @incollection{goncalves_neuroendocrinology_2017, title = {Neuroendocrinology of {Social} {Behavior} in {Teleost} {Fish}}, isbn = {978-0-12-803608-2}, url = {https://linkinghub.elsevier.com/retrieve/pii/B9780128035924000201}, language = {en}, urldate = {2021-02-19}, booktitle = {Hormones, {Brain} and {Behavior}}, publisher = {Elsevier}, author = {Gonçalves, David and Félix, Ana S. and Oliveira, Rui F.}, year = {2017}, doi = {10.1016/B978-0-12-803592-4.00020-1}, pages = {3--18}, } @techreport{goncalves_behavioral_2016, address = {Macao}, type = {Research {Report}}, title = {Behavioral plasticity meets neuroplasticity: brain neurogenesis in the polymorphic fish {Salaria} pavo}, institution = {University of Saint Joseph}, author = {Gonçalves, David Manuel Flores}, year = {2016}, } @article{fagundes_birth_2015, title = {Birth date predicts alternative life‐history pathways in a fish with sequential reproductive tactics}, volume = {29}, issn = {0269-8463, 1365-2435}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/1365-2435.12465}, doi = {10.1111/1365-2435.12465}, language = {en}, number = {12}, urldate = {2021-02-19}, journal = {Functional Ecology}, author = {Fagundes, Teresa and Simões, Mariana G. and Saraiva, João L. and Ros, Albert F. H. and Gonçalves, David and Oliveira, Rui F.}, editor = {Grindstaff, Jennifer}, month = dec, year = {2015}, note = {10 citations (Crossref) [2022-09-21]}, pages = {1533--1542}, } @article{goncalves_alternative_2014, title = {Alternative pathway history in a fish with plastic reproductive tactic}, journal = {Functional Ecology}, author = {Gonçalves, David}, year = {2014}, } @article{goncalves_oestradiol_2014, title = {Oestradiol and prostaglandin {F2α} regulate sexual display in female of a sex-role reversed fish}, volume = {281}, number = {1778}, author = {Gonçalves, David}, year = {2014}, } @article{cardoso_efficient_2013, title = {Efficient isolation of polymorphic microsatellites from high-throughput sequence data based on number of repeats}, volume = {11}, issn = {18747787}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1874778713000226}, doi = {10.1016/j.margen.2013.04.002}, language = {en}, urldate = {2021-02-19}, journal = {Marine Genomics}, author = {Cardoso, Sara D. and Gonçalves, David and Robalo, Joana I. and Almada, Vitor C. and Canário, Adelino V.M. and Oliveira, Rui F.}, month = sep, year = {2013}, note = {8 citations (Crossref) [2022-09-21]}, pages = {11--16}, } @article{saraiva_ecological_2013, title = {Ecological modulation of reproductive behaviour in the peacock blenny: a mini-review}, volume = {39}, issn = {0920-1742, 1573-5168}, shorttitle = {Ecological modulation of reproductive behaviour in the peacock blenny}, url = {http://link.springer.com/10.1007/s10695-012-9658-5}, doi = {10.1007/s10695-012-9658-5}, language = {en}, number = {1}, urldate = {2021-02-19}, journal = {Fish Physiology and Biochemistry}, author = {Saraiva, J. L. and Gonçalves, David and Oliveira, R. F.}, month = feb, year = {2013}, note = {6 citations (Crossref) [2022-09-21]}, pages = {85--89}, } @article{fagundes_social_2012, title = {Social cues in the expression of sequential alternative reproductive tactics in young males of the peacock blenny, {Salaria} pavo}, volume = {107}, issn = {00319384}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0031938412002740}, doi = {10.1016/j.physbeh.2012.07.011}, language = {en}, number = {3}, urldate = {2021-02-19}, journal = {Physiology \& Behavior}, author = {Fagundes, Teresa and Simões, Mariana G. and Gonçalves, David and Oliveira, Rui F.}, month = oct, year = {2012}, note = {4 citations (Crossref) [2022-09-21]}, pages = {283--291}, } @article{saraiva_interpopulational_2012, title = {Interpopulational variation of the mating system in the peacock blenny {Salaria} pavo}, volume = {15}, issn = {0873-9749, 1437-9546}, url = {http://link.springer.com/10.1007/s10211-011-0104-y}, doi = {10.1007/s10211-011-0104-y}, language = {en}, number = {1}, urldate = {2021-02-19}, journal = {acta ethologica}, author = {Saraiva, João L. and Pignolo, Giulia and Gonçalves, David and Oliveira, Rui F.}, month = apr, year = {2012}, note = {12 citations (Crossref) [2022-09-21]}, pages = {25--31}, } @incollection{goncalves_hormones_2011, title = {Hormones and {Sexual} {Behavior} of {Teleost} {Fishes}}, isbn = {978-0-12-375009-9}, url = {https://linkinghub.elsevier.com/retrieve/pii/B9780123750099100074}, language = {en}, urldate = {2021-02-19}, booktitle = {Hormones and {Reproduction} of {Vertebrates}}, publisher = {Elsevier}, author = {Gonçalves, David and Oliveira, Rui F.}, year = {2011}, doi = {10.1016/B978-0-12-375009-9.10007-4}, pages = {119--147}, } @article{oliveira_plasticity_2011, title = {Plasticity in reproductive behaviour in two populations of the peacock blenny}, volume = {148}, issn = {0005-7959, 1568-539X}, url = {https://brill.com/view/journals/beh/148/14/article-p1457_3.xml}, doi = {10.1163/156853911X617089}, number = {14}, urldate = {2021-02-19}, journal = {Behaviour}, author = {Oliveira, Rui F. and Simões, Mariana G. and Gonçalves, David and Saraiva, João L.}, year = {2011}, note = {3 citations (Crossref) [2022-09-21]}, pages = {1457--1472}, }