TY - JOUR TI - Examination of sex-related differences in intestinal and gonadal lipid metabolism in the sea cucumber Apostichopus japonicus AU - Zhang, Shuangyan AU - Ru, Xiaoshang AU - Zhang, Libin AU - Gonçalves, David AU - Yang, Hongsheng T2 - Aquaculture AB - Reproduction of the sea cucumber Apostichopus japonicus is critical for aquaculture production. Gonadal development is the basis of reproduction, and lipids, which are among the main nutrients required for gonadal development, directly affect reproduction. We investigated whether gonadal and intestinal lipid metabolism differed between male and female A. japonicus. Transcriptome analysis of the intestines of sexually mature male and female wild-caught individuals revealed differences in gene expression, with 27 and 39 genes being up-regulated in females and males, respectively. In particular, the expression of the fatty acid synthase gene was higher in males than in females. Metabolome analysis of the gonads identified 141 metabolites that were up-regulated and 175 metabolites that were down-regulated in the testes compared with the ovaries in the positive/negative mode of an LC-MS/MS analysis. A variety of polyunsaturated fatty acids were found at higher concentrations in the testes than in the ovaries. 16 s rDNA sequencing analysis showed that the composition and structure of the intestinal microbiota were similar between males and females. These results suggest that sex differences in intestinal metabolism of A. japonicus are not due to differences in the microbiota, and we speculate that gonadal metabolism may be related to intestinal morphology. This information might be useful in improving the reproductive efficiency of sea cucumbers in captivity. DA - 2023/01/15/ PY - 2023 DO - 10.1016/j.aquaculture.2022.738787 DP - ScienceDirect VL - 562 SP - 738787 J2 - Aquaculture LA - en SN - 0044-8486 UR - https://www.sciencedirect.com/science/article/pii/S0044848622009048 Y2 - 2023/04/11/10:01:48 KW - Gonads KW - Intestines KW - Lipid metabolism KW - Sea cucumber KW - Sex differences ER -