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Anthropogenic noise of variable temporal patterns is increasing in aquatic environments, causing physiological stress and sensory impairment. However, scarce information exists on exposure effects to continuous versus intermittent disturbances, which is critical for noise sustainable management. We tested the effects of different noise regimes on the auditory system and behaviour in the zebrafish (Danio rerio). Adult zebrafish were exposed for 24 h to either white noise (150 ± 10 dB re 1 μPa) or silent control. Acoustic playbacks varied in temporal patterns—continuous, fast and slow regular intermittent, and irregular intermittent. Auditory sensitivity was assessed with Auditory Evoked Potential recordings, revealing hearing loss and increased response latency in all noise-treated groups. The highest mean threshold shifts (c. 13 dB) were registered in continuous and fast intermittent treatments, and no differences were found between regular and irregular regimes. Inner ear saccule did not reveal significant hair cell loss but showed a decrease in presynaptic Ribeye b protein especially after continuous exposure. Behavioural assessment using the standardized Novel Tank Diving assay showed that all noise-treated fish spent > 98% time in the bottom within the first minute compared to 82% in control, indicating noise-induced anxiety/stress. We provide first data on how different noise time regimes impact a reference fish model, suggesting that overall acoustic energy is more important than regularity when predicting noise effects.
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Digital Factory (DF) planning is the key of intelligent factory construction, where intelligent production technologies of big data analysis, cloud computing, blockchain, Internet of Things, artificial intelligence, 5G, Time Sensitive Network (TSN), Digital Twin (DT), additive manufacturing are included. By applying the modern techniques, DF performs great advantages on the aspects of product lifecycle management, enterprise resource planning, operation management, supply chain management, real-time database construction, advanced process control, as well as the new technologies of distributed control system and fieldbus control system. This article delivers a review of key issues of DF top-level design and planning from the aspects of networking, precision, automation and digitalization. Solutions are explored based on 5G, TSN and DT advanced technologies, literately and practically. Additionally, the article describes the method and application of efficient big data comprehensive solution. Therefore, this study contributes valuable decision-making support for DF applications.
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